Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12320 and PS417_12325 are separated by 65 nucleotides PS417_12325 and PS417_12330 are separated by 70 nucleotides PS417_12330 and PS417_12335 overlap by 4 nucleotides
PS417_12320: PS417_12320 - amino acid ABC transporter substrate-binding protein, at 2,679,506 to 2,680,090
_12320
PS417_12325: PS417_12325 - precorrin-4 C11-methyltransferase, at 2,680,156 to 2,680,902
_12325
PS417_12330: PS417_12330 - cobalamin biosynthesis protein CobE, at 2,680,973 to 2,681,365
_12330
PS417_12335: PS417_12335 - cobalt transporter, at 2,681,362 to 2,682,066
_12335
Position (kb)
2680
2681 Strain fitness (log2 ratio)
-2
-1
0
1 at 2679.242 kb on + strand at 2679.242 kb on + strand at 2679.398 kb on - strand at 2679.404 kb on + strand at 2679.404 kb on + strand at 2679.404 kb on + strand at 2679.408 kb on + strand at 2679.408 kb on + strand at 2679.408 kb on + strand at 2679.409 kb on - strand at 2679.409 kb on - strand at 2679.409 kb on - strand at 2679.409 kb on - strand at 2680.085 kb on + strand at 2680.646 kb on + strand, within PS417_12325 at 2680.808 kb on + strand, within PS417_12325 at 2681.372 kb on + strand at 2681.613 kb on + strand, within PS417_12335 at 2681.614 kb on - strand, within PS417_12335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,679,242 + +0.3 2,679,242 + -0.5 2,679,398 - -0.3 2,679,404 + +1.7 2,679,404 + +1.0 2,679,404 + -0.1 2,679,408 + -0.8 2,679,408 + +0.4 2,679,408 + -0.4 2,679,409 - -0.0 2,679,409 - -0.3 2,679,409 - -0.2 2,679,409 - -0.8 2,680,085 + -1.9 2,680,646 + PS417_12325 0.66 -0.6 2,680,808 + PS417_12325 0.87 -0.8 2,681,372 + +0.3 2,681,613 + PS417_12335 0.36 -0.2 2,681,614 - PS417_12335 0.36 +0.3
Or see this region's nucleotide sequence