Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11435 and PS417_11440 are separated by 45 nucleotides PS417_11440 and PS417_11445 are separated by 80 nucleotides PS417_11445 and PS417_11450 are separated by 107 nucleotides
PS417_11435: PS417_11435 - asparagine synthase, at 2,460,673 to 2,462,577
_11435
PS417_11440: PS417_11440 - hypothetical protein, at 2,462,623 to 2,463,582
_11440
PS417_11445: PS417_11445 - enoyl-CoA hydratase, at 2,463,663 to 2,464,448
_11445
PS417_11450: PS417_11450 - AraC family transcriptional regulator, at 2,464,556 to 2,465,353
_11450
Position (kb)
2462
2463
2464 Strain fitness (log2 ratio)
-1
0
1 at 2461.929 kb on - strand, within PS417_11435 at 2462.129 kb on + strand, within PS417_11435 at 2462.130 kb on - strand, within PS417_11435 at 2462.253 kb on - strand, within PS417_11435 at 2462.303 kb on + strand, within PS417_11435 at 2462.334 kb on - strand at 2462.339 kb on + strand, within PS417_11435 at 2462.339 kb on + strand, within PS417_11435 at 2462.432 kb on + strand at 2462.567 kb on + strand at 2462.568 kb on - strand at 2462.568 kb on - strand at 2462.568 kb on - strand at 2462.568 kb on - strand at 2462.584 kb on + strand at 2462.584 kb on + strand at 2462.683 kb on + strand at 2462.683 kb on + strand at 2462.683 kb on + strand at 2463.020 kb on + strand, within PS417_11440 at 2463.332 kb on + strand, within PS417_11440 at 2463.333 kb on - strand, within PS417_11440 at 2463.599 kb on + strand at 2463.665 kb on - strand at 2463.949 kb on + strand, within PS417_11445 at 2463.950 kb on - strand, within PS417_11445 at 2464.001 kb on + strand, within PS417_11445 at 2464.036 kb on + strand, within PS417_11445 at 2464.037 kb on - strand, within PS417_11445 at 2464.289 kb on - strand, within PS417_11445 at 2464.317 kb on + strand, within PS417_11445 at 2464.417 kb on - strand at 2464.422 kb on + strand at 2464.536 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,461,929 - PS417_11435 0.66 +0.0 2,462,129 + PS417_11435 0.76 +0.1 2,462,130 - PS417_11435 0.76 +0.1 2,462,253 - PS417_11435 0.83 -0.4 2,462,303 + PS417_11435 0.86 -0.3 2,462,334 - -1.1 2,462,339 + PS417_11435 0.87 -0.3 2,462,339 + PS417_11435 0.87 +0.7 2,462,432 + -0.6 2,462,567 + -0.0 2,462,568 - +0.3 2,462,568 - +0.6 2,462,568 - -0.3 2,462,568 - -0.9 2,462,584 + +0.1 2,462,584 + -0.5 2,462,683 + +1.7 2,462,683 + +0.5 2,462,683 + +0.9 2,463,020 + PS417_11440 0.41 -0.6 2,463,332 + PS417_11440 0.74 -0.9 2,463,333 - PS417_11440 0.74 -0.5 2,463,599 + -0.1 2,463,665 - -1.0 2,463,949 + PS417_11445 0.36 -0.5 2,463,950 - PS417_11445 0.37 -0.2 2,464,001 + PS417_11445 0.43 +0.1 2,464,036 + PS417_11445 0.47 -0.1 2,464,037 - PS417_11445 0.48 +0.0 2,464,289 - PS417_11445 0.80 -0.8 2,464,317 + PS417_11445 0.83 -0.2 2,464,417 - -0.1 2,464,422 + -0.3 2,464,536 + -0.5
Or see this region's nucleotide sequence