Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09460 and PS417_09465 are separated by 9 nucleotides PS417_09465 and PS417_09470 are separated by 399 nucleotides PS417_09470 and PS417_09475 are separated by 233 nucleotides
PS417_09460: PS417_09460 - alkanesulfonate monooxygenase, at 2,048,616 to 2,049,758
_09460
PS417_09465: PS417_09465 - FMN reductase, at 2,049,768 to 2,050,331
_09465
PS417_09470: PS417_09470 - hypothetical protein, at 2,050,731 to 2,051,000
_09470
PS417_09475: PS417_09475 - class V aminotransferase, at 2,051,234 to 2,052,394
_09475
Position (kb)
2050
2051
2052 Strain fitness (log2 ratio)
-1
0
1 at 2049.849 kb on + strand, within PS417_09465 at 2049.849 kb on + strand, within PS417_09465 at 2049.850 kb on - strand, within PS417_09465 at 2049.850 kb on - strand, within PS417_09465 at 2049.850 kb on - strand, within PS417_09465 at 2049.852 kb on + strand, within PS417_09465 at 2049.853 kb on - strand, within PS417_09465 at 2049.864 kb on + strand, within PS417_09465 at 2049.899 kb on - strand, within PS417_09465 at 2050.081 kb on + strand, within PS417_09465 at 2050.251 kb on + strand, within PS417_09465 at 2050.539 kb on + strand at 2050.539 kb on + strand at 2050.541 kb on + strand at 2050.542 kb on - strand at 2050.542 kb on - strand at 2050.646 kb on + strand at 2050.686 kb on - strand at 2050.819 kb on - strand, within PS417_09470 at 2050.888 kb on - strand, within PS417_09470 at 2051.119 kb on - strand at 2051.119 kb on - strand at 2051.119 kb on - strand at 2051.122 kb on + strand at 2051.138 kb on + strand at 2051.239 kb on + strand at 2051.239 kb on + strand at 2051.252 kb on + strand at 2051.261 kb on + strand at 2051.603 kb on + strand, within PS417_09475 at 2051.886 kb on - strand, within PS417_09475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,049,849 + PS417_09465 0.14 +0.4 2,049,849 + PS417_09465 0.14 -0.5 2,049,850 - PS417_09465 0.15 -0.8 2,049,850 - PS417_09465 0.15 -0.0 2,049,850 - PS417_09465 0.15 +0.1 2,049,852 + PS417_09465 0.15 +0.3 2,049,853 - PS417_09465 0.15 +0.2 2,049,864 + PS417_09465 0.17 -0.3 2,049,899 - PS417_09465 0.23 -0.2 2,050,081 + PS417_09465 0.55 +1.7 2,050,251 + PS417_09465 0.86 +1.0 2,050,539 + +0.6 2,050,539 + +0.2 2,050,541 + +0.8 2,050,542 - -1.4 2,050,542 - +0.2 2,050,646 + +0.0 2,050,686 - -0.8 2,050,819 - PS417_09470 0.33 -1.2 2,050,888 - PS417_09470 0.58 -1.4 2,051,119 - +1.4 2,051,119 - +0.0 2,051,119 - -0.2 2,051,122 + +0.6 2,051,138 + +0.3 2,051,239 + -0.3 2,051,239 + +0.3 2,051,252 + +0.4 2,051,261 + -0.5 2,051,603 + PS417_09475 0.32 +0.2 2,051,886 - PS417_09475 0.56 +0.1
Or see this region's nucleotide sequence