Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08460 and PS417_08465 are separated by 121 nucleotides PS417_08465 and PS417_08470 are separated by 5 nucleotides PS417_08470 and PS417_08475 are separated by 132 nucleotides
PS417_08460: PS417_08460 - CysB family transcriptional regulator, at 1,851,249 to 1,852,223
_08460
PS417_08465: PS417_08465 - elongation factor GreAB, at 1,852,345 to 1,852,827
_08465
PS417_08470: PS417_08470 - hypothetical protein, at 1,852,833 to 1,853,201
_08470
PS417_08475: PS417_08475 - hypothetical protein, at 1,853,334 to 1,854,467
_08475
Position (kb)
1852
1853 Strain fitness (log2 ratio)
-1
0
1 at 1851.841 kb on + strand at 1851.946 kb on - strand at 1851.946 kb on - strand, within PS417_08460 at 1852.221 kb on + strand at 1852.245 kb on + strand at 1852.246 kb on - strand at 1852.253 kb on - strand at 1852.339 kb on + strand at 1852.351 kb on - strand at 1852.360 kb on - strand at 1852.365 kb on + strand at 1852.366 kb on - strand at 1852.592 kb on + strand, within PS417_08465 at 1852.593 kb on - strand, within PS417_08465 at 1852.593 kb on - strand, within PS417_08465 at 1852.701 kb on - strand, within PS417_08465 at 1852.701 kb on - strand, within PS417_08465 at 1852.701 kb on - strand, within PS417_08465 at 1852.821 kb on + strand at 1852.822 kb on - strand at 1852.822 kb on - strand at 1852.929 kb on - strand, within PS417_08470 at 1852.993 kb on + strand, within PS417_08470 at 1853.095 kb on + strand, within PS417_08470 at 1853.096 kb on - strand, within PS417_08470 at 1853.296 kb on + strand at 1853.296 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,851,841 + -1.1 1,851,946 - -1.4 1,851,946 - PS417_08460 0.71 +0.4 1,852,221 + -0.1 1,852,245 + +0.0 1,852,246 - -0.6 1,852,253 - -0.7 1,852,339 + +0.4 1,852,351 - +0.6 1,852,360 - -1.3 1,852,365 + +0.1 1,852,366 - -0.1 1,852,592 + PS417_08465 0.51 +1.0 1,852,593 - PS417_08465 0.51 +1.4 1,852,593 - PS417_08465 0.51 +0.0 1,852,701 - PS417_08465 0.74 +0.3 1,852,701 - PS417_08465 0.74 +1.6 1,852,701 - PS417_08465 0.74 +0.3 1,852,821 + +0.0 1,852,822 - -1.7 1,852,822 - -0.1 1,852,929 - PS417_08470 0.26 -0.0 1,852,993 + PS417_08470 0.43 +0.2 1,853,095 + PS417_08470 0.71 +0.3 1,853,096 - PS417_08470 0.71 +0.6 1,853,296 + -0.2 1,853,296 + +0.3
Or see this region's nucleotide sequence