Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06135 and PS417_06140 are separated by 182 nucleotides PS417_06140 and PS417_06145 are separated by 139 nucleotides PS417_06145 and PS417_06150 are separated by 54 nucleotides
PS417_06135: PS417_06135 - glycerol-3-phosphate acyltransferase, at 1,359,056 to 1,361,575
_06135
PS417_06140: PS417_06140 - cold-shock protein, at 1,361,758 to 1,361,967
_06140
PS417_06145: PS417_06145 - hypothetical protein, at 1,362,107 to 1,362,484
_06145
PS417_06150: PS417_06150 - SAM-dependent methlyltransferase, at 1,362,539 to 1,363,348
_06150
Position (kb)
1361
1362 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1360.779 kb on + strand, within PS417_06135 at 1360.780 kb on - strand, within PS417_06135 at 1360.780 kb on - strand, within PS417_06135 at 1360.780 kb on - strand, within PS417_06135 at 1360.780 kb on - strand, within PS417_06135 at 1360.786 kb on - strand, within PS417_06135 at 1361.005 kb on + strand, within PS417_06135 at 1361.020 kb on + strand, within PS417_06135 at 1361.099 kb on + strand, within PS417_06135 at 1361.099 kb on + strand, within PS417_06135 at 1361.112 kb on - strand, within PS417_06135 at 1361.445 kb on + strand at 1361.446 kb on - strand at 1361.446 kb on - strand at 1361.446 kb on - strand at 1361.521 kb on - strand at 1361.550 kb on - strand at 1361.575 kb on + strand at 1361.750 kb on + strand at 1361.810 kb on - strand, within PS417_06140 at 1361.836 kb on - strand, within PS417_06140 at 1361.854 kb on + strand, within PS417_06140 at 1361.911 kb on + strand, within PS417_06140 at 1361.912 kb on - strand, within PS417_06140 at 1362.295 kb on - strand, within PS417_06145 at 1362.536 kb on + strand at 1362.541 kb on - strand at 1362.768 kb on + strand, within PS417_06150 at 1362.769 kb on - strand, within PS417_06150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,360,779 + PS417_06135 0.68 +0.8 1,360,780 - PS417_06135 0.68 +0.4 1,360,780 - PS417_06135 0.68 +0.6 1,360,780 - PS417_06135 0.68 +0.6 1,360,780 - PS417_06135 0.68 +0.4 1,360,786 - PS417_06135 0.69 -0.1 1,361,005 + PS417_06135 0.77 +1.3 1,361,020 + PS417_06135 0.78 +0.9 1,361,099 + PS417_06135 0.81 +1.8 1,361,099 + PS417_06135 0.81 +0.8 1,361,112 - PS417_06135 0.82 +0.4 1,361,445 + +1.5 1,361,446 - +0.2 1,361,446 - -1.0 1,361,446 - +0.4 1,361,521 - +1.8 1,361,550 - -0.9 1,361,575 + +2.8 1,361,750 + +1.2 1,361,810 - PS417_06140 0.25 -1.6 1,361,836 - PS417_06140 0.37 +0.4 1,361,854 + PS417_06140 0.46 -1.1 1,361,911 + PS417_06140 0.73 -0.2 1,361,912 - PS417_06140 0.73 -1.4 1,362,295 - PS417_06145 0.50 -1.0 1,362,536 + +0.2 1,362,541 - +0.1 1,362,768 + PS417_06150 0.28 +0.0 1,362,769 - PS417_06150 0.28 -0.4
Or see this region's nucleotide sequence