Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05095 and PS417_05100 overlap by 4 nucleotides PS417_05100 and PS417_05105 are separated by 170 nucleotides PS417_05105 and PS417_05110 overlap by 4 nucleotides
PS417_05095: PS417_05095 - membrane protein, at 1,133,013 to 1,133,954
_05095
PS417_05100: PS417_05100 - RNA polymerase sigma factor, at 1,133,951 to 1,134,439
_05100
PS417_05105: PS417_05105 - membrane protein, at 1,134,610 to 1,134,912
_05105
PS417_05110: PS417_05110 - peptidase, at 1,134,909 to 1,136,483
_05110
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-2
-1
0
1 at 1133.119 kb on + strand, within PS417_05095 at 1133.344 kb on + strand, within PS417_05095 at 1133.344 kb on + strand, within PS417_05095 at 1133.394 kb on - strand, within PS417_05095 at 1133.657 kb on + strand, within PS417_05095 at 1133.658 kb on - strand at 1133.658 kb on - strand, within PS417_05095 at 1133.658 kb on - strand, within PS417_05095 at 1133.954 kb on + strand at 1134.016 kb on + strand, within PS417_05100 at 1134.016 kb on + strand, within PS417_05100 at 1134.202 kb on - strand, within PS417_05100 at 1134.408 kb on + strand at 1134.408 kb on + strand at 1134.408 kb on + strand at 1134.409 kb on - strand at 1134.471 kb on - strand at 1134.641 kb on - strand, within PS417_05105 at 1134.787 kb on + strand, within PS417_05105 at 1134.787 kb on + strand, within PS417_05105 at 1134.788 kb on - strand, within PS417_05105 at 1134.788 kb on - strand, within PS417_05105 at 1135.011 kb on + strand at 1135.012 kb on - strand at 1135.101 kb on + strand, within PS417_05110 at 1135.101 kb on + strand, within PS417_05110 at 1135.102 kb on - strand, within PS417_05110 at 1135.294 kb on - strand, within PS417_05110
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,133,119 + PS417_05095 0.11 -0.3 1,133,344 + PS417_05095 0.35 +0.8 1,133,344 + PS417_05095 0.35 -0.2 1,133,394 - PS417_05095 0.40 -0.3 1,133,657 + PS417_05095 0.68 +0.8 1,133,658 - +0.2 1,133,658 - PS417_05095 0.68 -0.3 1,133,658 - PS417_05095 0.68 -1.1 1,133,954 + -0.7 1,134,016 + PS417_05100 0.13 -0.5 1,134,016 + PS417_05100 0.13 -1.1 1,134,202 - PS417_05100 0.51 -1.4 1,134,408 + +0.2 1,134,408 + -0.1 1,134,408 + +0.3 1,134,409 - +1.6 1,134,471 - -0.5 1,134,641 - PS417_05105 0.10 -0.1 1,134,787 + PS417_05105 0.58 -2.2 1,134,787 + PS417_05105 0.58 +0.1 1,134,788 - PS417_05105 0.59 +0.2 1,134,788 - PS417_05105 0.59 +0.5 1,135,011 + +0.2 1,135,012 - -0.5 1,135,101 + PS417_05110 0.12 -0.1 1,135,101 + PS417_05110 0.12 +0.1 1,135,102 - PS417_05110 0.12 +1.2 1,135,294 - PS417_05110 0.24 -0.1
Or see this region's nucleotide sequence