Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00465 and PS417_00470 are separated by 728 nucleotides PS417_00470 and PS417_00475 are separated by 98 nucleotides
PS417_00465: PS417_00465 - hypothetical protein, at 91,480 to 91,947
_00465
PS417_00470: PS417_00470 - glutamate:protein symporter, at 92,676 to 94,007
_00470
PS417_00475: PS417_00475 - membrane protein, at 94,106 to 95,335
_00475
Position (kb) 92
93
94
95 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 91.714 kb on + strand at 91.714 kb on + strand, within PS417_00465 at 91.714 kb on + strand at 91.715 kb on - strand, within PS417_00465 at 91.715 kb on - strand, within PS417_00465 at 91.801 kb on + strand, within PS417_00465 at 91.801 kb on + strand, within PS417_00465 at 91.802 kb on - strand, within PS417_00465 at 91.843 kb on - strand, within PS417_00465 at 91.935 kb on + strand at 91.936 kb on - strand at 91.936 kb on - strand at 91.942 kb on + strand at 91.942 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 92.090 kb on + strand at 92.090 kb on + strand at 92.185 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.208 kb on - strand at 92.208 kb on - strand at 92.254 kb on + strand at 92.254 kb on + strand at 92.258 kb on + strand at 92.259 kb on - strand at 92.279 kb on + strand at 92.279 kb on + strand at 92.280 kb on - strand at 92.531 kb on + strand at 92.531 kb on + strand at 92.557 kb on + strand at 92.648 kb on + strand at 92.663 kb on + strand at 92.663 kb on + strand at 92.663 kb on + strand at 92.669 kb on + strand at 92.693 kb on - strand at 92.813 kb on + strand, within PS417_00470 at 92.814 kb on - strand, within PS417_00470 at 92.814 kb on - strand, within PS417_00470 at 92.935 kb on - strand, within PS417_00470 at 93.020 kb on + strand, within PS417_00470 at 93.049 kb on - strand, within PS417_00470 at 93.122 kb on + strand, within PS417_00470 at 93.289 kb on - strand, within PS417_00470 at 93.495 kb on + strand, within PS417_00470 at 93.628 kb on - strand, within PS417_00470 at 93.628 kb on - strand, within PS417_00470 at 93.663 kb on + strand, within PS417_00470 at 93.663 kb on + strand, within PS417_00470 at 93.664 kb on - strand, within PS417_00470 at 93.664 kb on - strand, within PS417_00470 at 93.731 kb on + strand, within PS417_00470 at 93.732 kb on - strand, within PS417_00470 at 93.915 kb on + strand at 93.915 kb on + strand at 93.915 kb on + strand at 93.915 kb on + strand at 93.915 kb on + strand at 93.916 kb on - strand at 93.916 kb on - strand at 93.916 kb on - strand at 94.072 kb on + strand at 94.725 kb on - strand, within PS417_00475 at 94.725 kb on - strand, within PS417_00475 at 94.886 kb on + strand, within PS417_00475 at 94.909 kb on + strand, within PS417_00475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 91,714 + -0.9 91,714 + PS417_00465 0.50 -0.4 91,714 + +0.3 91,715 - PS417_00465 0.50 -1.1 91,715 - PS417_00465 0.50 -1.1 91,801 + PS417_00465 0.69 -0.1 91,801 + PS417_00465 0.69 -0.5 91,802 - PS417_00465 0.69 +0.3 91,843 - PS417_00465 0.78 -0.2 91,935 + +0.8 91,936 - +0.2 91,936 - -0.4 91,942 + +0.1 91,942 + -0.2 91,945 + +0.2 91,945 + +0.2 91,945 + +1.2 91,945 + +2.1 91,945 + +0.0 91,946 - -0.7 91,946 - -0.2 91,946 - +1.3 91,946 - +0.1 91,946 - -0.6 92,090 + +0.5 92,090 + +0.8 92,185 + +0.5 92,207 + -0.4 92,207 + -1.3 92,207 + +0.6 92,207 + -0.2 92,208 - -0.1 92,208 - +0.2 92,254 + -1.7 92,254 + -0.4 92,258 + -0.9 92,259 - +0.8 92,279 + -0.1 92,279 + -0.5 92,280 - -0.9 92,531 + -1.1 92,531 + -1.2 92,557 + +0.6 92,648 + -1.8 92,663 + -0.1 92,663 + +0.2 92,663 + +0.9 92,669 + -0.2 92,693 - +0.3 92,813 + PS417_00470 0.10 -1.5 92,814 - PS417_00470 0.10 +1.0 92,814 - PS417_00470 0.10 -0.2 92,935 - PS417_00470 0.19 +0.6 93,020 + PS417_00470 0.26 +0.0 93,049 - PS417_00470 0.28 -0.2 93,122 + PS417_00470 0.33 +1.2 93,289 - PS417_00470 0.46 +0.6 93,495 + PS417_00470 0.61 +0.1 93,628 - PS417_00470 0.71 -1.4 93,628 - PS417_00470 0.71 -1.6 93,663 + PS417_00470 0.74 +2.5 93,663 + PS417_00470 0.74 +1.6 93,664 - PS417_00470 0.74 +0.6 93,664 - PS417_00470 0.74 -0.1 93,731 + PS417_00470 0.79 -0.8 93,732 - PS417_00470 0.79 +0.2 93,915 + +0.6 93,915 + +0.3 93,915 + +0.2 93,915 + +1.3 93,915 + +0.6 93,916 - +0.6 93,916 - +0.1 93,916 - +0.2 94,072 + -0.0 94,725 - PS417_00475 0.50 -0.6 94,725 - PS417_00475 0.50 -1.8 94,886 + PS417_00475 0.63 -2.8 94,909 + PS417_00475 0.65 -1.8
Or see this region's nucleotide sequence