Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23315 and PS417_23320 are separated by 195 nucleotides PS417_23320 and PS417_23325 overlap by 4 nucleotides PS417_23325 and PS417_23330 are separated by 197 nucleotides PS417_23330 and PS417_23335 are separated by 101 nucleotides
PS417_23315: PS417_23315 - transcriptional regulator, at 5,046,228 to 5,046,608
_23315
PS417_23320: PS417_23320 - heme oxygenase, at 5,046,804 to 5,047,418
_23320
PS417_23325: PS417_23325 - histidine kinase, at 5,047,415 to 5,049,658
_23325
PS417_23330: PS417_23330 - hypothetical protein, at 5,049,856 to 5,050,152
_23330
PS417_23335: PS417_23335 - isoleucyl-tRNA synthetase, at 5,050,254 to 5,052,140
_23335
Position (kb) 5047
5048
5049
5050 Strain fitness (log2 ratio) -1
0
1
2 at 5046.489 kb on - strand, within PS417_23315 at 5046.719 kb on - strand at 5046.723 kb on + strand at 5046.724 kb on - strand at 5046.741 kb on - strand at 5046.741 kb on - strand at 5046.933 kb on + strand, within PS417_23320 at 5046.934 kb on - strand, within PS417_23320 at 5046.955 kb on - strand, within PS417_23320 at 5046.955 kb on - strand, within PS417_23320 at 5046.958 kb on - strand, within PS417_23320 at 5046.958 kb on - strand, within PS417_23320 at 5046.958 kb on - strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.259 kb on + strand, within PS417_23320 at 5047.263 kb on + strand, within PS417_23320 at 5047.264 kb on - strand, within PS417_23320 at 5047.264 kb on - strand, within PS417_23320 at 5047.408 kb on + strand at 5047.408 kb on + strand at 5047.409 kb on - strand at 5047.409 kb on - strand at 5047.660 kb on + strand, within PS417_23325 at 5047.660 kb on + strand, within PS417_23325 at 5047.660 kb on + strand, within PS417_23325 at 5047.661 kb on - strand, within PS417_23325 at 5047.661 kb on - strand, within PS417_23325 at 5047.919 kb on + strand, within PS417_23325 at 5048.181 kb on - strand, within PS417_23325 at 5048.181 kb on - strand, within PS417_23325 at 5048.631 kb on - strand, within PS417_23325 at 5049.182 kb on + strand, within PS417_23325 at 5049.182 kb on + strand, within PS417_23325 at 5049.183 kb on - strand, within PS417_23325 at 5049.204 kb on + strand, within PS417_23325 at 5049.204 kb on + strand, within PS417_23325 at 5049.539 kb on + strand at 5049.539 kb on + strand at 5049.540 kb on - strand at 5049.540 kb on - strand at 5049.540 kb on - strand at 5049.553 kb on + strand at 5049.554 kb on - strand at 5049.682 kb on + strand at 5049.683 kb on - strand at 5049.693 kb on + strand at 5049.766 kb on - strand at 5049.780 kb on - strand at 5049.838 kb on + strand at 5049.839 kb on - strand at 5049.839 kb on - strand at 5049.857 kb on + strand at 5049.859 kb on + strand at 5049.860 kb on - strand at 5049.860 kb on - strand at 5049.882 kb on + strand at 5049.883 kb on - strand at 5049.924 kb on + strand, within PS417_23330 at 5049.924 kb on + strand, within PS417_23330 at 5050.074 kb on + strand at 5050.075 kb on - strand, within PS417_23330 at 5050.153 kb on - strand at 5050.153 kb on - strand at 5050.189 kb on - strand at 5050.391 kb on + strand at 5050.551 kb on + strand, within PS417_23335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 5,046,489 - PS417_23315 0.69 +0.3 5,046,719 - +0.0 5,046,723 + +1.6 5,046,724 - +0.4 5,046,741 - -0.1 5,046,741 - -1.7 5,046,933 + PS417_23320 0.21 +0.3 5,046,934 - PS417_23320 0.21 +0.3 5,046,955 - PS417_23320 0.25 +2.2 5,046,955 - PS417_23320 0.25 +1.4 5,046,958 - PS417_23320 0.25 -0.2 5,046,958 - PS417_23320 0.25 +0.0 5,046,958 - PS417_23320 0.25 -0.8 5,047,161 + PS417_23320 0.58 +0.2 5,047,161 + PS417_23320 0.58 -0.2 5,047,161 + PS417_23320 0.58 -0.5 5,047,161 + PS417_23320 0.58 +0.6 5,047,161 + PS417_23320 0.58 +0.0 5,047,161 + PS417_23320 0.58 -0.1 5,047,161 + PS417_23320 0.58 -0.2 5,047,259 + PS417_23320 0.74 +0.2 5,047,263 + PS417_23320 0.75 +1.0 5,047,264 - PS417_23320 0.75 +0.1 5,047,264 - PS417_23320 0.75 +0.2 5,047,408 + -0.5 5,047,408 + +0.6 5,047,409 - +0.2 5,047,409 - -0.8 5,047,660 + PS417_23325 0.11 +0.2 5,047,660 + PS417_23325 0.11 -0.2 5,047,660 + PS417_23325 0.11 -0.2 5,047,661 - PS417_23325 0.11 -0.6 5,047,661 - PS417_23325 0.11 +0.5 5,047,919 + PS417_23325 0.22 -0.3 5,048,181 - PS417_23325 0.34 -0.8 5,048,181 - PS417_23325 0.34 -0.6 5,048,631 - PS417_23325 0.54 -1.0 5,049,182 + PS417_23325 0.79 +0.1 5,049,182 + PS417_23325 0.79 -0.9 5,049,183 - PS417_23325 0.79 -1.1 5,049,204 + PS417_23325 0.80 -0.9 5,049,204 + PS417_23325 0.80 -1.1 5,049,539 + +0.1 5,049,539 + +0.0 5,049,540 - -0.2 5,049,540 - +0.0 5,049,540 - +0.5 5,049,553 + -0.2 5,049,554 - -0.5 5,049,682 + -0.3 5,049,683 - +0.1 5,049,693 + +0.6 5,049,766 - +0.4 5,049,780 - +0.6 5,049,838 + -0.1 5,049,839 - -0.1 5,049,839 - +0.6 5,049,857 + +0.6 5,049,859 + +0.3 5,049,860 - +0.1 5,049,860 - -0.9 5,049,882 + -0.8 5,049,883 - -1.5 5,049,924 + PS417_23330 0.23 -0.1 5,049,924 + PS417_23330 0.23 -0.2 5,050,074 + +0.1 5,050,075 - PS417_23330 0.74 +0.5 5,050,153 - +0.5 5,050,153 - -0.3 5,050,189 - +1.0 5,050,391 + +1.1 5,050,551 + PS417_23335 0.16 -0.5
Or see this region's nucleotide sequence