Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00365 and PS417_00370 are separated by 98 nucleotides PS417_00370 and PS417_00375 are separated by 37 nucleotides PS417_00375 and PS417_00380 overlap by 8 nucleotides PS417_00380 and PS417_00385 overlap by 1 nucleotides
PS417_00365: PS417_00365 - hydroperoxidase, at 70,467 to 72,608
_00365
PS417_00370: PS417_00370 - hypothetical protein, at 72,707 to 73,426
_00370
PS417_00375: PS417_00375 - membrane protein, at 73,464 to 74,252
_00375
PS417_00380: PS417_00380 - zinc ABC transporter ATP-binding protein, at 74,245 to 75,030
_00380
PS417_00385: PS417_00385 - Fur family transcriptional regulator, at 75,030 to 75,512
_00385
Position (kb) 73
74
75 Strain fitness (log2 ratio) -1
0
1
2 at 72.584 kb on + strand at 72.585 kb on - strand at 72.591 kb on - strand at 73.183 kb on - strand, within PS417_00370 at 73.440 kb on + strand at 73.465 kb on + strand at 73.887 kb on - strand, within PS417_00375 at 73.913 kb on - strand, within PS417_00375 at 74.155 kb on + strand, within PS417_00375 at 74.262 kb on + strand at 74.348 kb on + strand, within PS417_00380 at 74.550 kb on - strand at 74.550 kb on - strand, within PS417_00380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 72,584 + -0.4 72,585 - +2.1 72,591 - -0.3 73,183 - PS417_00370 0.66 +0.6 73,440 + +0.3 73,465 + +0.7 73,887 - PS417_00375 0.54 -0.9 73,913 - PS417_00375 0.57 -1.5 74,155 + PS417_00375 0.88 -0.0 74,262 + -0.1 74,348 + PS417_00380 0.13 -0.6 74,550 - -1.3 74,550 - PS417_00380 0.39 -0.3
Or see this region's nucleotide sequence