Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28215 and PS417_28220 are separated by 196 nucleotides PS417_28220 and PS417_28225 overlap by 23 nucleotides PS417_28225 and PS417_28230 are separated by 17 nucleotides
PS417_28215: PS417_28215 - pyruvate carboxylase subunit A, at 6,091,754 to 6,093,169
_28215
PS417_28220: PS417_28220 - LysR family transcriptional regulator, at 6,093,366 to 6,094,340
_28220
PS417_28225: PS417_28225 - hypothetical protein, at 6,094,318 to 6,094,611
_28225
PS417_28230: PS417_28230 - transcriptional regulator, at 6,094,629 to 6,095,495
_28230
Position (kb)
6093
6094
6095 Strain fitness (log2 ratio)
-2
-1
0
1 at 6093.387 kb on - strand at 6093.439 kb on - strand at 6093.439 kb on - strand at 6093.949 kb on - strand, within PS417_28220 at 6093.949 kb on - strand, within PS417_28220 at 6094.075 kb on + strand, within PS417_28220 at 6094.559 kb on + strand, within PS417_28225 at 6094.795 kb on + strand, within PS417_28230 at 6094.795 kb on + strand at 6094.796 kb on - strand, within PS417_28230 at 6094.796 kb on - strand, within PS417_28230 at 6095.095 kb on + strand, within PS417_28230 at 6095.096 kb on - strand, within PS417_28230 at 6095.215 kb on + strand, within PS417_28230 at 6095.216 kb on - strand, within PS417_28230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 6,093,387 - -0.4 6,093,439 - +0.4 6,093,439 - +0.5 6,093,949 - PS417_28220 0.60 -0.3 6,093,949 - PS417_28220 0.60 +1.1 6,094,075 + PS417_28220 0.73 +0.7 6,094,559 + PS417_28225 0.82 -0.6 6,094,795 + PS417_28230 0.19 -1.8 6,094,795 + -0.1 6,094,796 - PS417_28230 0.19 -0.4 6,094,796 - PS417_28230 0.19 +0.2 6,095,095 + PS417_28230 0.54 -0.6 6,095,096 - PS417_28230 0.54 +0.5 6,095,215 + PS417_28230 0.68 +0.5 6,095,216 - PS417_28230 0.68 -0.8
Or see this region's nucleotide sequence