Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27775 and PS417_27780 are separated by 5 nucleotides PS417_27780 and PS417_27785 are separated by 264 nucleotides PS417_27785 and PS417_27790 are separated by 19 nucleotides
PS417_27775: PS417_27775 - phosphopantothenoylcysteine decarboxylase, at 5,989,772 to 5,990,980
_27775
PS417_27780: PS417_27780 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 5,990,986 to 5,991,441
_27780
PS417_27785: PS417_27785 - phosphoglucomutase, at 5,991,706 to 5,993,103
_27785
PS417_27790: PS417_27790 - acetylglutamate kinase, at 5,993,123 to 5,994,028
_27790
Position (kb) 5991
5992
5993
5994 Strain fitness (log2 ratio) -2
-1
0
1 at 5991.444 kb on - strand at 5991.630 kb on + strand at 5991.631 kb on - strand at 5991.761 kb on - strand at 5991.982 kb on + strand, within PS417_27785 at 5991.998 kb on + strand, within PS417_27785 at 5991.999 kb on - strand, within PS417_27785 at 5991.999 kb on - strand, within PS417_27785 at 5992.186 kb on + strand, within PS417_27785 at 5992.616 kb on + strand, within PS417_27785 at 5992.737 kb on + strand, within PS417_27785 at 5992.738 kb on - strand, within PS417_27785 at 5992.738 kb on - strand, within PS417_27785 at 5992.738 kb on - strand, within PS417_27785 at 5992.741 kb on + strand, within PS417_27785 at 5992.742 kb on - strand, within PS417_27785 at 5992.821 kb on + strand, within PS417_27785 at 5993.093 kb on + strand at 5993.094 kb on - strand at 5993.195 kb on + strand at 5993.196 kb on - strand at 5993.867 kb on + strand, within PS417_27790 at 5994.027 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 5,991,444 - +0.1 5,991,630 + -1.8 5,991,631 - -0.1 5,991,761 - -0.2 5,991,982 + PS417_27785 0.20 +0.9 5,991,998 + PS417_27785 0.21 -0.4 5,991,999 - PS417_27785 0.21 -0.8 5,991,999 - PS417_27785 0.21 -1.1 5,992,186 + PS417_27785 0.34 +0.5 5,992,616 + PS417_27785 0.65 +0.5 5,992,737 + PS417_27785 0.74 +0.5 5,992,738 - PS417_27785 0.74 -1.0 5,992,738 - PS417_27785 0.74 -1.1 5,992,738 - PS417_27785 0.74 -1.2 5,992,741 + PS417_27785 0.74 -0.6 5,992,742 - PS417_27785 0.74 -0.1 5,992,821 + PS417_27785 0.80 -0.9 5,993,093 + +0.5 5,993,094 - -0.1 5,993,195 + -0.1 5,993,196 - +0.0 5,993,867 + PS417_27790 0.82 +0.9 5,994,027 - -0.1
Or see this region's nucleotide sequence