Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25805 and PS417_25810 overlap by 4 nucleotides PS417_25810 and PS417_25815 are separated by 97 nucleotides PS417_25815 and PS417_25820 overlap by 1 nucleotides PS417_25820 and PS417_25825 are separated by 9 nucleotides
PS417_25805: PS417_25805 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 5,572,864 to 5,573,853
_25805
PS417_25810: PS417_25810 - ribosomal RNA small subunit methyltransferase A, at 5,573,850 to 5,574,662
_25810
PS417_25815: PS417_25815 - magnesium transporter ApaG, at 5,574,760 to 5,575,140
_25815
PS417_25820: PS417_25820 - diadenosine tetraphosphatase, at 5,575,140 to 5,575,991
_25820
PS417_25825: PS417_25825 - sulfurtransferase, at 5,576,001 to 5,576,330
_25825
Position (kb) 5574
5575
5576 Strain fitness (log2 ratio) -2
-1
0
1 at 5573.849 kb on + strand at 5574.675 kb on - strand at 5574.675 kb on - strand at 5575.002 kb on + strand, within PS417_25815 at 5575.003 kb on - strand, within PS417_25815 at 5575.003 kb on - strand, within PS417_25815 at 5575.006 kb on + strand, within PS417_25815 at 5575.006 kb on + strand, within PS417_25815 at 5575.006 kb on + strand, within PS417_25815 at 5575.006 kb on + strand, within PS417_25815 at 5575.007 kb on - strand, within PS417_25815 at 5575.934 kb on + strand at 5575.935 kb on - strand at 5575.965 kb on - strand at 5575.989 kb on + strand at 5576.018 kb on + strand at 5576.018 kb on + strand at 5576.091 kb on + strand, within PS417_25825
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 5,573,849 + -0.7 5,574,675 - +0.1 5,574,675 - -0.0 5,575,002 + PS417_25815 0.64 -2.3 5,575,003 - PS417_25815 0.64 +0.9 5,575,003 - PS417_25815 0.64 +0.9 5,575,006 + PS417_25815 0.65 -0.3 5,575,006 + PS417_25815 0.65 +0.4 5,575,006 + PS417_25815 0.65 +1.1 5,575,006 + PS417_25815 0.65 +1.5 5,575,007 - PS417_25815 0.65 +0.7 5,575,934 + -0.1 5,575,935 - +1.4 5,575,965 - +0.8 5,575,989 + +0.2 5,576,018 + -0.0 5,576,018 + +0.0 5,576,091 + PS417_25825 0.27 +0.5
Or see this region's nucleotide sequence