Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01955 and PS417_01960 are separated by 218 nucleotides PS417_01960 and PS417_01965 overlap by 4 nucleotides PS417_01965 and PS417_01970 overlap by 4 nucleotides
PS417_01955: PS417_01955 - peptidase, at 433,512 to 435,974
_01955
PS417_01960: PS417_01960 - pilus assembly protein, at 436,193 to 436,846
_01960
PS417_01965: PS417_01965 - fimbriae biosynthesis protein, at 436,843 to 437,397
_01965
PS417_01970: PS417_01970 - pilus assembly protein, at 437,394 to 438,353
_01970
Position (kb) 436
437
438 Strain fitness (log2 ratio) -2
-1
0
1
2 at 435.884 kb on + strand at 436.007 kb on - strand at 436.082 kb on + strand at 436.083 kb on - strand at 436.166 kb on - strand at 436.166 kb on - strand at 436.311 kb on - strand, within PS417_01960 at 436.375 kb on - strand, within PS417_01960 at 436.536 kb on - strand, within PS417_01960 at 436.536 kb on - strand, within PS417_01960 at 436.617 kb on + strand, within PS417_01960 at 436.617 kb on + strand, within PS417_01960 at 436.618 kb on - strand at 436.618 kb on - strand, within PS417_01960 at 436.862 kb on + strand at 436.862 kb on + strand at 436.863 kb on - strand at 436.903 kb on + strand, within PS417_01965 at 436.907 kb on + strand, within PS417_01965 at 436.907 kb on + strand, within PS417_01965 at 437.453 kb on - strand at 437.517 kb on + strand, within PS417_01970 at 437.517 kb on + strand, within PS417_01970 at 437.518 kb on - strand, within PS417_01970 at 437.950 kb on - strand, within PS417_01970 at 437.980 kb on + strand, within PS417_01970 at 438.181 kb on + strand, within PS417_01970 at 438.182 kb on - strand, within PS417_01970 at 438.182 kb on - strand, within PS417_01970 at 438.338 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 435,884 + -2.4 436,007 - +1.1 436,082 + -1.4 436,083 - +0.0 436,166 - -0.1 436,166 - -0.7 436,311 - PS417_01960 0.18 +0.1 436,375 - PS417_01960 0.28 +0.6 436,536 - PS417_01960 0.52 +0.3 436,536 - PS417_01960 0.52 -0.4 436,617 + PS417_01960 0.65 +0.4 436,617 + PS417_01960 0.65 -0.3 436,618 - +1.5 436,618 - PS417_01960 0.65 +0.5 436,862 + -0.5 436,862 + -0.3 436,863 - -1.1 436,903 + PS417_01965 0.11 +2.0 436,907 + PS417_01965 0.12 +0.2 436,907 + PS417_01965 0.12 +0.5 437,453 - +0.7 437,517 + PS417_01970 0.13 +1.0 437,517 + PS417_01970 0.13 -0.8 437,518 - PS417_01970 0.13 -0.4 437,950 - PS417_01970 0.58 -0.4 437,980 + PS417_01970 0.61 -0.8 438,181 + PS417_01970 0.82 +0.2 438,182 - PS417_01970 0.82 -0.6 438,182 - PS417_01970 0.82 -0.6 438,338 - -1.3
Or see this region's nucleotide sequence