Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01570 and PS417_01575 are separated by 39 nucleotides PS417_01575 and PS417_01580 are separated by 9 nucleotides PS417_01580 and PS417_01585 are separated by 66 nucleotides PS417_01585 and PS417_01590 are separated by 50 nucleotides
PS417_01570: PS417_01570 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, at 347,390 to 347,647
_01570
PS417_01575: PS417_01575 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 347,687 to 348,424
_01575
PS417_01580: PS417_01580 - imidazole glycerol phosphate synthase, at 348,434 to 349,204
_01580
PS417_01585: PS417_01585 - amino acid ABC transporter substrate-binding protein, at 349,271 to 350,026
_01585
PS417_01590: PS417_01590 - polysaccharide deacetylase, at 350,077 to 350,850
_01590
Position (kb) 348
349
350 Strain fitness (log2 ratio) -1
0
1
2 at 347.492 kb on - strand, within PS417_01570 at 347.558 kb on + strand, within PS417_01570 at 347.559 kb on - strand, within PS417_01570 at 347.730 kb on + strand at 347.806 kb on - strand, within PS417_01575 at 347.964 kb on - strand, within PS417_01575 at 347.985 kb on - strand, within PS417_01575 at 348.365 kb on + strand at 348.365 kb on + strand at 348.366 kb on - strand at 348.414 kb on - strand at 348.578 kb on - strand, within PS417_01580 at 348.578 kb on - strand, within PS417_01580 at 349.263 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.269 kb on - strand at 349.269 kb on - strand at 349.269 kb on - strand at 349.269 kb on - strand at 349.269 kb on - strand at 349.303 kb on + strand at 349.491 kb on - strand, within PS417_01585 at 349.526 kb on + strand, within PS417_01585 at 349.526 kb on + strand, within PS417_01585 at 349.527 kb on - strand, within PS417_01585 at 349.527 kb on - strand, within PS417_01585 at 349.709 kb on + strand, within PS417_01585 at 349.710 kb on - strand at 349.710 kb on - strand, within PS417_01585 at 349.753 kb on - strand, within PS417_01585 at 349.848 kb on - strand, within PS417_01585 at 349.914 kb on - strand, within PS417_01585 at 350.018 kb on + strand at 350.018 kb on + strand at 350.018 kb on + strand at 350.019 kb on - strand at 350.066 kb on - strand at 350.149 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 347,492 - PS417_01570 0.40 -0.2 347,558 + PS417_01570 0.65 +0.4 347,559 - PS417_01570 0.66 -0.4 347,730 + +0.6 347,806 - PS417_01575 0.16 +1.1 347,964 - PS417_01575 0.38 +0.6 347,985 - PS417_01575 0.40 +0.3 348,365 + +1.0 348,365 + +0.5 348,366 - +0.2 348,414 - +0.5 348,578 - PS417_01580 0.19 +0.7 348,578 - PS417_01580 0.19 +0.7 349,263 + -0.0 349,268 + +0.8 349,268 + +0.2 349,268 + +0.5 349,268 + +0.2 349,268 + +0.1 349,268 + +1.4 349,269 - +1.2 349,269 - +0.2 349,269 - +0.3 349,269 - +1.0 349,269 - -0.8 349,303 + +0.6 349,491 - PS417_01585 0.29 +0.3 349,526 + PS417_01585 0.34 +0.9 349,526 + PS417_01585 0.34 +1.0 349,527 - PS417_01585 0.34 -0.1 349,527 - PS417_01585 0.34 -0.2 349,709 + PS417_01585 0.58 -0.4 349,710 - -1.3 349,710 - PS417_01585 0.58 +0.8 349,753 - PS417_01585 0.64 +0.5 349,848 - PS417_01585 0.76 +1.4 349,914 - PS417_01585 0.85 +1.3 350,018 + +1.9 350,018 + -0.2 350,018 + +1.0 350,019 - -0.1 350,066 - -0.5 350,149 - -1.2
Or see this region's nucleotide sequence