Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15175 and PS417_15180 overlap by 4 nucleotides PS417_15180 and PS417_15185 are separated by 3 nucleotides
PS417_15175: PS417_15175 - acetyl-CoA acetyltransferase, at 3,325,543 to 3,326,841
_15175
PS417_15180: PS417_15180 - acyl dehydratase, at 3,326,838 to 3,327,728
_15180
PS417_15185: PS417_15185 - 3-ketoacyl-ACP reductase, at 3,327,732 to 3,329,075
_15185
Position (kb)
3326
3327
3328 Strain fitness (log2 ratio)
-1
0
1
2 at 3326.690 kb on + strand, within PS417_15175 at 3326.691 kb on - strand, within PS417_15175 at 3326.750 kb on + strand at 3327.189 kb on - strand, within PS417_15180 at 3327.192 kb on + strand, within PS417_15180 at 3327.192 kb on + strand, within PS417_15180 at 3327.193 kb on - strand, within PS417_15180 at 3327.193 kb on - strand, within PS417_15180 at 3327.193 kb on - strand, within PS417_15180 at 3327.368 kb on - strand, within PS417_15180 at 3327.368 kb on - strand at 3327.515 kb on - strand, within PS417_15180 at 3327.721 kb on + strand at 3327.721 kb on + strand at 3327.722 kb on - strand at 3327.996 kb on + strand, within PS417_15185 at 3328.019 kb on - strand, within PS417_15185 at 3328.022 kb on + strand, within PS417_15185 at 3328.215 kb on - strand, within PS417_15185 at 3328.512 kb on + strand, within PS417_15185 at 3328.512 kb on + strand, within PS417_15185 at 3328.527 kb on + strand, within PS417_15185 at 3328.528 kb on - strand, within PS417_15185 at 3328.531 kb on + strand, within PS417_15185 at 3328.532 kb on - strand, within PS417_15185 at 3328.532 kb on - strand at 3328.600 kb on + strand, within PS417_15185 at 3328.601 kb on - strand, within PS417_15185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,326,690 + PS417_15175 0.88 -0.1 3,326,691 - PS417_15175 0.88 -1.4 3,326,750 + +0.5 3,327,189 - PS417_15180 0.39 +0.0 3,327,192 + PS417_15180 0.40 -1.0 3,327,192 + PS417_15180 0.40 -0.9 3,327,193 - PS417_15180 0.40 +0.3 3,327,193 - PS417_15180 0.40 -1.5 3,327,193 - PS417_15180 0.40 -0.3 3,327,368 - PS417_15180 0.59 +0.0 3,327,368 - +0.0 3,327,515 - PS417_15180 0.76 -0.2 3,327,721 + -0.2 3,327,721 + +1.4 3,327,722 - +0.8 3,327,996 + PS417_15185 0.20 -0.3 3,328,019 - PS417_15185 0.21 +0.0 3,328,022 + PS417_15185 0.22 +0.1 3,328,215 - PS417_15185 0.36 +2.0 3,328,512 + PS417_15185 0.58 -0.2 3,328,512 + PS417_15185 0.58 +0.6 3,328,527 + PS417_15185 0.59 -0.1 3,328,528 - PS417_15185 0.59 -0.8 3,328,531 + PS417_15185 0.59 -1.3 3,328,532 - PS417_15185 0.60 -0.1 3,328,532 - +0.3 3,328,600 + PS417_15185 0.65 -0.1 3,328,601 - PS417_15185 0.65 +0.6
Or see this region's nucleotide sequence