Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13615 and PS417_13620 overlap by 11 nucleotides PS417_13620 and PS417_13625 are separated by 181 nucleotides PS417_13625 and PS417_13635 are separated by 164 nucleotides
PS417_13615: PS417_13615 - long-chain acyl-CoA synthetase, at 2,997,271 to 2,998,749
_13615
PS417_13620: PS417_13620 - thermostable hemolysin, at 2,998,739 to 2,999,413
_13620
PS417_13625: PS417_13625 - cytochrome B561, at 2,999,595 to 3,000,137
_13625
PS417_13635: PS417_13635 - D-ribose transporter ATP-binding protein, at 3,000,302 to 3,001,834
_13635
Position (kb)
2998
2999
3000 Strain fitness (log2 ratio)
-1
0
1 at 2998.332 kb on + strand, within PS417_13615 at 2998.649 kb on + strand at 2998.863 kb on + strand, within PS417_13620 at 2998.864 kb on - strand, within PS417_13620 at 2999.357 kb on - strand at 2999.622 kb on + strand at 2999.622 kb on + strand at 2999.623 kb on - strand at 2999.679 kb on + strand, within PS417_13625 at 2999.680 kb on - strand, within PS417_13625 at 2999.867 kb on + strand, within PS417_13625 at 2999.868 kb on - strand, within PS417_13625 at 2999.883 kb on + strand, within PS417_13625 at 2999.884 kb on - strand, within PS417_13625 at 3000.217 kb on + strand at 3000.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,998,332 + PS417_13615 0.72 -0.6 2,998,649 + +1.3 2,998,863 + PS417_13620 0.18 -0.1 2,998,864 - PS417_13620 0.19 -1.2 2,999,357 - -0.4 2,999,622 + +0.1 2,999,622 + -0.7 2,999,623 - -0.4 2,999,679 + PS417_13625 0.15 -0.9 2,999,680 - PS417_13625 0.16 -0.8 2,999,867 + PS417_13625 0.50 +1.0 2,999,868 - PS417_13625 0.50 -0.3 2,999,883 + PS417_13625 0.53 -0.3 2,999,884 - PS417_13625 0.53 +0.3 3,000,217 + +0.9 3,000,218 - -0.2
Or see this region's nucleotide sequence