Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07620 and PS417_07625 are separated by 49 nucleotides PS417_07625 and PS417_07630 are separated by 9 nucleotides PS417_07630 and PS417_07635 are separated by 11 nucleotides
PS417_07620: PS417_07620 - beta-hexosaminidase, at 1,676,293 to 1,677,303
_07620
PS417_07625: PS417_07625 - helicase, at 1,677,353 to 1,680,046
_07625
PS417_07630: PS417_07630 - hypothetical protein, at 1,680,056 to 1,680,622
_07630
PS417_07635: PS417_07635 - transcription-repair coupling factor, at 1,680,634 to 1,684,083
_07635
Position (kb)
1677
1678
1679
1680
1681 Strain fitness (log2 ratio)
-2
-1
0
1 at 1676.579 kb on - strand, within PS417_07620 at 1676.938 kb on + strand, within PS417_07620 at 1677.447 kb on - strand at 1677.751 kb on - strand, within PS417_07625 at 1677.868 kb on + strand, within PS417_07625 at 1677.868 kb on + strand, within PS417_07625 at 1678.050 kb on + strand, within PS417_07625 at 1678.051 kb on - strand, within PS417_07625 at 1678.389 kb on + strand, within PS417_07625 at 1678.464 kb on + strand, within PS417_07625 at 1678.465 kb on - strand, within PS417_07625 at 1678.486 kb on - strand, within PS417_07625 at 1678.486 kb on - strand, within PS417_07625 at 1678.486 kb on - strand, within PS417_07625 at 1678.513 kb on + strand, within PS417_07625 at 1678.513 kb on + strand at 1678.514 kb on - strand, within PS417_07625 at 1678.527 kb on + strand, within PS417_07625 at 1678.527 kb on + strand, within PS417_07625 at 1678.528 kb on - strand, within PS417_07625 at 1678.605 kb on - strand, within PS417_07625 at 1678.950 kb on + strand, within PS417_07625 at 1678.950 kb on + strand, within PS417_07625 at 1678.993 kb on - strand, within PS417_07625 at 1679.168 kb on - strand, within PS417_07625 at 1679.168 kb on - strand, within PS417_07625 at 1679.523 kb on + strand, within PS417_07625 at 1679.523 kb on + strand, within PS417_07625 at 1679.523 kb on + strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.535 kb on + strand, within PS417_07625 at 1679.535 kb on + strand, within PS417_07625 at 1679.569 kb on - strand, within PS417_07625 at 1679.990 kb on - strand at 1680.147 kb on + strand, within PS417_07630 at 1680.393 kb on + strand, within PS417_07630 at 1680.393 kb on + strand, within PS417_07630 at 1680.393 kb on + strand, within PS417_07630 at 1680.394 kb on - strand, within PS417_07630 at 1680.394 kb on - strand, within PS417_07630 at 1680.394 kb on - strand, within PS417_07630 at 1680.424 kb on + strand, within PS417_07630 at 1680.427 kb on + strand, within PS417_07630 at 1680.428 kb on - strand, within PS417_07630 at 1680.452 kb on - strand, within PS417_07630 at 1680.452 kb on - strand, within PS417_07630 at 1680.452 kb on - strand, within PS417_07630 at 1680.678 kb on + strand at 1680.678 kb on + strand at 1680.678 kb on + strand at 1680.678 kb on + strand at 1680.678 kb on + strand at 1680.679 kb on - strand at 1680.682 kb on - strand at 1680.682 kb on - strand at 1680.682 kb on - strand at 1680.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,676,579 - PS417_07620 0.28 -0.3 1,676,938 + PS417_07620 0.64 +1.7 1,677,447 - +0.2 1,677,751 - PS417_07625 0.15 -0.0 1,677,868 + PS417_07625 0.19 -0.4 1,677,868 + PS417_07625 0.19 -1.0 1,678,050 + PS417_07625 0.26 -0.8 1,678,051 - PS417_07625 0.26 +0.1 1,678,389 + PS417_07625 0.38 -2.3 1,678,464 + PS417_07625 0.41 +0.3 1,678,465 - PS417_07625 0.41 +0.1 1,678,486 - PS417_07625 0.42 -0.1 1,678,486 - PS417_07625 0.42 -0.4 1,678,486 - PS417_07625 0.42 +0.1 1,678,513 + PS417_07625 0.43 -1.1 1,678,513 + -0.8 1,678,514 - PS417_07625 0.43 -0.3 1,678,527 + PS417_07625 0.44 -1.6 1,678,527 + PS417_07625 0.44 -0.4 1,678,528 - PS417_07625 0.44 -1.4 1,678,605 - PS417_07625 0.46 -0.3 1,678,950 + PS417_07625 0.59 -0.1 1,678,950 + PS417_07625 0.59 +0.3 1,678,993 - PS417_07625 0.61 +0.5 1,679,168 - PS417_07625 0.67 -0.6 1,679,168 - PS417_07625 0.67 +0.5 1,679,523 + PS417_07625 0.81 +0.6 1,679,523 + PS417_07625 0.81 +0.2 1,679,523 + PS417_07625 0.81 -0.2 1,679,524 - PS417_07625 0.81 -1.3 1,679,524 - PS417_07625 0.81 -0.2 1,679,524 - PS417_07625 0.81 +0.7 1,679,524 - PS417_07625 0.81 -0.4 1,679,524 - PS417_07625 0.81 -0.5 1,679,524 - PS417_07625 0.81 -0.2 1,679,524 - PS417_07625 0.81 -0.4 1,679,535 + PS417_07625 0.81 -0.2 1,679,535 + PS417_07625 0.81 -0.1 1,679,569 - PS417_07625 0.82 -0.1 1,679,990 - +0.1 1,680,147 + PS417_07630 0.16 -0.8 1,680,393 + PS417_07630 0.59 -0.1 1,680,393 + PS417_07630 0.59 -1.0 1,680,393 + PS417_07630 0.59 -0.4 1,680,394 - PS417_07630 0.60 -0.8 1,680,394 - PS417_07630 0.60 +0.5 1,680,394 - PS417_07630 0.60 -0.4 1,680,424 + PS417_07630 0.65 -0.2 1,680,427 + PS417_07630 0.65 +0.1 1,680,428 - PS417_07630 0.66 +0.0 1,680,452 - PS417_07630 0.70 -0.1 1,680,452 - PS417_07630 0.70 -0.1 1,680,452 - PS417_07630 0.70 -0.1 1,680,678 + -0.1 1,680,678 + -0.6 1,680,678 + +0.6 1,680,678 + +0.1 1,680,678 + -0.4 1,680,679 - +0.6 1,680,682 - +0.5 1,680,682 - +0.9 1,680,682 - -0.6 1,680,729 + +0.2
Or see this region's nucleotide sequence