Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07465 and PS417_07470 are separated by 176 nucleotides PS417_07470 and PS417_07475 overlap by 4 nucleotides PS417_07475 and PS417_07480 overlap by 4 nucleotides
PS417_07465: PS417_07465 - LysR family transcriptional regulator, at 1,641,627 to 1,642,565
_07465
PS417_07470: PS417_07470 - hypothetical protein, at 1,642,742 to 1,643,482
_07470
PS417_07475: PS417_07475 - allophanate hydrolase, at 1,643,479 to 1,644,183
_07475
PS417_07480: PS417_07480 - allophanate hydrolase, at 1,644,180 to 1,645,088
_07480
Position (kb) 1642
1643
1644 Strain fitness (log2 ratio) -1
0
1
2 at 1641.991 kb on + strand, within PS417_07465 at 1641.992 kb on - strand, within PS417_07465 at 1642.046 kb on - strand at 1642.046 kb on - strand, within PS417_07465 at 1642.046 kb on - strand, within PS417_07465 at 1642.419 kb on + strand, within PS417_07465 at 1642.420 kb on - strand, within PS417_07465 at 1642.693 kb on + strand at 1642.708 kb on + strand at 1642.725 kb on + strand at 1643.063 kb on + strand, within PS417_07470 at 1643.217 kb on - strand, within PS417_07470 at 1643.223 kb on + strand, within PS417_07470 at 1643.415 kb on + strand at 1643.857 kb on + strand, within PS417_07475 at 1644.035 kb on + strand, within PS417_07475 at 1644.146 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,641,991 + PS417_07465 0.39 +0.1 1,641,992 - PS417_07465 0.39 +0.5 1,642,046 - +2.5 1,642,046 - PS417_07465 0.45 +0.7 1,642,046 - PS417_07465 0.45 -1.0 1,642,419 + PS417_07465 0.84 +1.2 1,642,420 - PS417_07465 0.84 +0.6 1,642,693 + -0.3 1,642,708 + -0.3 1,642,725 + +0.1 1,643,063 + PS417_07470 0.43 +1.9 1,643,217 - PS417_07470 0.64 +0.6 1,643,223 + PS417_07470 0.65 +0.4 1,643,415 + +0.0 1,643,857 + PS417_07475 0.54 +1.8 1,644,035 + PS417_07475 0.79 -0.1 1,644,146 + +2.1
Or see this region's nucleotide sequence