Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06960 and PS417_06965 are separated by 84 nucleotides PS417_06965 and PS417_06970 are separated by 207 nucleotides PS417_06970 and PS417_06975 are separated by 95 nucleotides
PS417_06960: PS417_06960 - membrane protein, at 1,539,470 to 1,539,895
_06960
PS417_06965: PS417_06965 - cysteine methyltransferase, at 1,539,980 to 1,540,480
_06965
PS417_06970: PS417_06970 - LysR family transcriptional regulator, at 1,540,688 to 1,541,581
_06970
PS417_06975: PS417_06975 - membrane protein, at 1,541,677 to 1,542,840
_06975
Position (kb)
1540
1541
1542 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1539.751 kb on + strand, within PS417_06960 at 1539.752 kb on - strand, within PS417_06960 at 1539.752 kb on - strand, within PS417_06960 at 1539.752 kb on - strand, within PS417_06960 at 1539.962 kb on + strand at 1540.299 kb on + strand, within PS417_06965 at 1540.299 kb on + strand, within PS417_06965 at 1540.353 kb on + strand, within PS417_06965 at 1540.354 kb on - strand, within PS417_06965 at 1540.468 kb on - strand at 1540.505 kb on + strand at 1540.516 kb on + strand at 1540.517 kb on - strand at 1540.541 kb on + strand at 1540.542 kb on - strand at 1540.542 kb on - strand at 1540.633 kb on - strand at 1540.654 kb on - strand at 1540.690 kb on - strand at 1540.809 kb on + strand, within PS417_06970 at 1540.810 kb on - strand, within PS417_06970 at 1541.289 kb on + strand, within PS417_06970 at 1541.289 kb on + strand, within PS417_06970 at 1541.570 kb on + strand at 1541.643 kb on + strand at 1541.643 kb on + strand at 1541.643 kb on + strand at 1541.661 kb on + strand at 1541.746 kb on + strand at 1541.746 kb on + strand at 1541.747 kb on - strand at 1541.747 kb on - strand at 1541.747 kb on - strand at 1541.747 kb on - strand at 1541.747 kb on - strand at 1541.947 kb on + strand, within PS417_06975 at 1542.031 kb on + strand, within PS417_06975 at 1542.032 kb on - strand, within PS417_06975 at 1542.082 kb on + strand, within PS417_06975 at 1542.083 kb on - strand, within PS417_06975 at 1542.083 kb on - strand, within PS417_06975 at 1542.298 kb on + strand, within PS417_06975 at 1542.298 kb on + strand, within PS417_06975 at 1542.299 kb on - strand, within PS417_06975 at 1542.299 kb on - strand, within PS417_06975 at 1542.299 kb on - strand, within PS417_06975 at 1542.306 kb on - strand, within PS417_06975 at 1542.343 kb on - strand, within PS417_06975 at 1542.407 kb on + strand, within PS417_06975 at 1542.407 kb on + strand, within PS417_06975 at 1542.407 kb on + strand, within PS417_06975 at 1542.407 kb on + strand, within PS417_06975 at 1542.407 kb on + strand, within PS417_06975 at 1542.407 kb on + strand, within PS417_06975 at 1542.408 kb on - strand, within PS417_06975 at 1542.408 kb on - strand, within PS417_06975 at 1542.408 kb on - strand at 1542.408 kb on - strand, within PS417_06975 at 1542.408 kb on - strand, within PS417_06975 at 1542.409 kb on + strand, within PS417_06975 at 1542.409 kb on + strand, within PS417_06975 at 1542.410 kb on - strand, within PS417_06975 at 1542.410 kb on - strand, within PS417_06975 at 1542.410 kb on - strand, within PS417_06975 at 1542.410 kb on - strand, within PS417_06975 at 1542.411 kb on + strand, within PS417_06975 at 1542.411 kb on + strand, within PS417_06975 at 1542.411 kb on + strand, within PS417_06975 at 1542.411 kb on + strand, within PS417_06975 at 1542.412 kb on - strand, within PS417_06975 at 1542.412 kb on - strand, within PS417_06975 at 1542.412 kb on - strand, within PS417_06975
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,539,751 + PS417_06960 0.66 +0.1 1,539,752 - PS417_06960 0.66 -0.1 1,539,752 - PS417_06960 0.66 -0.2 1,539,752 - PS417_06960 0.66 +0.7 1,539,962 + -0.3 1,540,299 + PS417_06965 0.64 -0.5 1,540,299 + PS417_06965 0.64 -0.2 1,540,353 + PS417_06965 0.74 +0.7 1,540,354 - PS417_06965 0.75 -0.6 1,540,468 - -0.4 1,540,505 + +0.2 1,540,516 + -2.2 1,540,517 - -0.2 1,540,541 + +0.1 1,540,542 - +0.7 1,540,542 - -0.5 1,540,633 - -0.5 1,540,654 - +0.8 1,540,690 - -0.8 1,540,809 + PS417_06970 0.14 +1.5 1,540,810 - PS417_06970 0.14 +0.1 1,541,289 + PS417_06970 0.67 +0.2 1,541,289 + PS417_06970 0.67 +0.9 1,541,570 + +1.5 1,541,643 + +0.8 1,541,643 + -0.0 1,541,643 + +3.1 1,541,661 + -0.7 1,541,746 + -0.7 1,541,746 + -0.8 1,541,747 - +0.2 1,541,747 - -0.4 1,541,747 - +0.4 1,541,747 - +0.8 1,541,747 - +0.1 1,541,947 + PS417_06975 0.23 -0.9 1,542,031 + PS417_06975 0.30 -0.0 1,542,032 - PS417_06975 0.30 -0.8 1,542,082 + PS417_06975 0.35 -0.7 1,542,083 - PS417_06975 0.35 -0.3 1,542,083 - PS417_06975 0.35 +0.5 1,542,298 + PS417_06975 0.53 -1.0 1,542,298 + PS417_06975 0.53 +1.6 1,542,299 - PS417_06975 0.53 -0.9 1,542,299 - PS417_06975 0.53 -0.1 1,542,299 - PS417_06975 0.53 -0.2 1,542,306 - PS417_06975 0.54 +0.2 1,542,343 - PS417_06975 0.57 -0.7 1,542,407 + PS417_06975 0.63 -0.5 1,542,407 + PS417_06975 0.63 -1.6 1,542,407 + PS417_06975 0.63 +0.6 1,542,407 + PS417_06975 0.63 +0.6 1,542,407 + PS417_06975 0.63 -0.5 1,542,407 + PS417_06975 0.63 -0.4 1,542,408 - PS417_06975 0.63 -0.3 1,542,408 - PS417_06975 0.63 -0.0 1,542,408 - -0.1 1,542,408 - PS417_06975 0.63 -0.1 1,542,408 - PS417_06975 0.63 -0.0 1,542,409 + PS417_06975 0.63 -0.7 1,542,409 + PS417_06975 0.63 -0.0 1,542,410 - PS417_06975 0.63 +1.1 1,542,410 - PS417_06975 0.63 -0.8 1,542,410 - PS417_06975 0.63 -2.8 1,542,410 - PS417_06975 0.63 +1.0 1,542,411 + PS417_06975 0.63 +0.0 1,542,411 + PS417_06975 0.63 -0.5 1,542,411 + PS417_06975 0.63 -1.6 1,542,411 + PS417_06975 0.63 -0.3 1,542,412 - PS417_06975 0.63 +0.9 1,542,412 - PS417_06975 0.63 -0.6 1,542,412 - PS417_06975 0.63 +0.5
Or see this region's nucleotide sequence