Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04895 and PS417_04900 are separated by 346 nucleotides PS417_04900 and PS417_04905 are separated by 72 nucleotides
PS417_04895: PS417_04895 - carboxylesterase, at 1,090,770 to 1,091,426
_04895
PS417_04900: PS417_04900 - amino acid ABC transporter substrate-binding protein, at 1,091,773 to 1,092,804
_04900
PS417_04905: PS417_04905 - amino acid ABC transporter permease, at 1,092,877 to 1,094,058
_04905
Position (kb)
1091
1092
1093 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1090.809 kb on + strand at 1090.810 kb on - strand at 1090.843 kb on + strand, within PS417_04895 at 1090.945 kb on - strand, within PS417_04895 at 1091.005 kb on + strand, within PS417_04895 at 1091.006 kb on - strand, within PS417_04895 at 1091.006 kb on - strand, within PS417_04895 at 1091.119 kb on - strand, within PS417_04895 at 1091.242 kb on + strand, within PS417_04895 at 1091.376 kb on + strand at 1091.376 kb on + strand at 1091.459 kb on - strand at 1091.496 kb on - strand at 1091.577 kb on + strand at 1091.577 kb on + strand at 1091.583 kb on + strand at 1091.584 kb on - strand at 1091.620 kb on - strand at 1091.718 kb on + strand at 1091.718 kb on + strand at 1091.718 kb on + strand at 1091.719 kb on - strand at 1091.719 kb on - strand at 1091.781 kb on - strand at 1092.149 kb on - strand, within PS417_04900 at 1092.149 kb on - strand, within PS417_04900 at 1092.151 kb on + strand, within PS417_04900 at 1092.151 kb on + strand, within PS417_04900 at 1092.241 kb on - strand, within PS417_04900 at 1092.292 kb on + strand, within PS417_04900 at 1092.293 kb on - strand, within PS417_04900 at 1092.293 kb on - strand, within PS417_04900 at 1092.293 kb on - strand, within PS417_04900 at 1092.421 kb on + strand, within PS417_04900 at 1092.422 kb on - strand, within PS417_04900 at 1092.518 kb on - strand, within PS417_04900 at 1092.666 kb on - strand, within PS417_04900 at 1092.666 kb on - strand, within PS417_04900 at 1092.691 kb on + strand, within PS417_04900 at 1092.692 kb on - strand, within PS417_04900 at 1092.692 kb on - strand, within PS417_04900 at 1092.784 kb on + strand at 1092.785 kb on - strand at 1093.033 kb on - strand, within PS417_04905 at 1093.115 kb on - strand, within PS417_04905 at 1093.209 kb on - strand, within PS417_04905 at 1093.255 kb on + strand, within PS417_04905 at 1093.255 kb on + strand, within PS417_04905 at 1093.256 kb on - strand, within PS417_04905 at 1093.256 kb on - strand at 1093.687 kb on + strand, within PS417_04905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,090,809 + +1.4 1,090,810 - -1.9 1,090,843 + PS417_04895 0.11 +1.7 1,090,945 - PS417_04895 0.27 -0.9 1,091,005 + PS417_04895 0.36 -0.2 1,091,006 - PS417_04895 0.36 -2.2 1,091,006 - PS417_04895 0.36 -2.9 1,091,119 - PS417_04895 0.53 -0.4 1,091,242 + PS417_04895 0.72 -0.8 1,091,376 + -2.8 1,091,376 + -2.7 1,091,459 - +1.8 1,091,496 - -0.7 1,091,577 + +0.2 1,091,577 + -0.2 1,091,583 + +0.4 1,091,584 - +0.4 1,091,620 - +0.2 1,091,718 + +1.1 1,091,718 + -2.2 1,091,718 + +0.4 1,091,719 - +0.3 1,091,719 - -1.4 1,091,781 - -0.1 1,092,149 - PS417_04900 0.36 -2.3 1,092,149 - PS417_04900 0.36 +0.1 1,092,151 + PS417_04900 0.37 +3.3 1,092,151 + PS417_04900 0.37 +3.3 1,092,241 - PS417_04900 0.45 +1.8 1,092,292 + PS417_04900 0.50 -0.5 1,092,293 - PS417_04900 0.50 +2.6 1,092,293 - PS417_04900 0.50 -1.1 1,092,293 - PS417_04900 0.50 +0.3 1,092,421 + PS417_04900 0.63 +0.8 1,092,422 - PS417_04900 0.63 +0.5 1,092,518 - PS417_04900 0.72 +1.9 1,092,666 - PS417_04900 0.87 +0.3 1,092,666 - PS417_04900 0.87 -1.9 1,092,691 + PS417_04900 0.89 +0.4 1,092,692 - PS417_04900 0.89 +0.2 1,092,692 - PS417_04900 0.89 +0.4 1,092,784 + +0.8 1,092,785 - -0.7 1,093,033 - PS417_04905 0.13 -2.0 1,093,115 - PS417_04905 0.20 -0.9 1,093,209 - PS417_04905 0.28 -1.7 1,093,255 + PS417_04905 0.32 +0.6 1,093,255 + PS417_04905 0.32 -1.1 1,093,256 - PS417_04905 0.32 +0.7 1,093,256 - +1.5 1,093,687 + PS417_04905 0.69 -0.4
Or see this region's nucleotide sequence