Strain Fitness in Pseudomonas simiae WCS417 around PS417_04500

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_04490 and PS417_04495 are separated by 133 nucleotidesPS417_04495 and PS417_04500 are separated by 11 nucleotidesPS417_04500 and PS417_04505 are separated by 36 nucleotidesPS417_04505 and PS417_04510 are separated by 131 nucleotides PS417_04490: PS417_04490 - glycerol acyltransferase, at 992,799 to 993,962 _04490 PS417_04495: PS417_04495 - LITAF-like zinc ribbon domain containing protein, at 994,096 to 994,278 _04495 PS417_04500: PS417_04500 - hypothetical protein, at 994,290 to 994,550 _04500 PS417_04505: PS417_04505 - hypothetical protein, at 994,587 to 995,207 _04505 PS417_04510: PS417_04510 - chemotaxis protein, at 995,339 to 997,477 _04510 Position (kb) 994 995Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 993.977 kb on - strandat 993.977 kb on - strandat 993.992 kb on - strandat 994.005 kb on - strandat 994.005 kb on - strandat 994.024 kb on - strandat 994.024 kb on - strandat 994.042 kb on - strandat 994.042 kb on - strandat 994.052 kb on - strandat 994.056 kb on - strandat 994.056 kb on - strandat 994.070 kb on - strandat 994.070 kb on - strandat 994.070 kb on - strandat 994.115 kb on - strand, within PS417_04495at 994.115 kb on - strand, within PS417_04495at 994.115 kb on - strand, within PS417_04495at 994.115 kb on - strand, within PS417_04495at 994.127 kb on - strand, within PS417_04495at 994.180 kb on + strand, within PS417_04495at 994.180 kb on + strand, within PS417_04495at 994.180 kb on + strand, within PS417_04495at 994.180 kb on + strand, within PS417_04495at 994.180 kb on + strand, within PS417_04495at 994.181 kb on - strand, within PS417_04495at 994.181 kb on - strand, within PS417_04495at 994.181 kb on - strand, within PS417_04495at 994.181 kb on - strand, within PS417_04495at 994.181 kb on - strand, within PS417_04495at 994.181 kb on - strand, within PS417_04495at 994.181 kb on - strand, within PS417_04495at 994.182 kb on + strand, within PS417_04495at 994.183 kb on - strand, within PS417_04495at 994.183 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.185 kb on - strand, within PS417_04495at 994.284 kb on - strandat 994.284 kb on - strandat 994.284 kb on - strandat 994.303 kb on - strandat 994.485 kb on + strand, within PS417_04500at 994.485 kb on + strand, within PS417_04500at 994.548 kb on + strandat 994.549 kb on - strandat 995.272 kb on + strandat 995.279 kb on + strandat 995.279 kb on + strandat 995.280 kb on - strandat 995.280 kb on - strandat 995.280 kb on - strandat 995.285 kb on + strandat 995.285 kb on + strandat 995.285 kb on + strandat 995.286 kb on - strandat 995.286 kb on - strandat 995.286 kb on - strandat 995.286 kb on - strandat 995.286 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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993,977 - +2.3
993,977 - -1.7
993,992 - +0.3
994,005 - +0.2
994,005 - +1.5
994,024 - +0.2
994,024 - -0.8
994,042 - +0.0
994,042 - +1.9
994,052 - +0.4
994,056 - -2.0
994,056 - +0.7
994,070 - +0.5
994,070 - +1.6
994,070 - +1.0
994,115 - PS417_04495 0.10 +2.2
994,115 - PS417_04495 0.10 +0.7
994,115 - PS417_04495 0.10 +0.6
994,115 - PS417_04495 0.10 +0.7
994,127 - PS417_04495 0.17 -1.5
994,180 + PS417_04495 0.46 +1.3
994,180 + PS417_04495 0.46 +0.3
994,180 + PS417_04495 0.46 +1.1
994,180 + PS417_04495 0.46 -1.3
994,180 + PS417_04495 0.46 +0.9
994,181 - PS417_04495 0.46 +1.0
994,181 - PS417_04495 0.46 -2.2
994,181 - PS417_04495 0.46 +0.4
994,181 - PS417_04495 0.46 +0.4
994,181 - PS417_04495 0.46 +0.9
994,181 - PS417_04495 0.46 +0.9
994,181 - PS417_04495 0.46 -1.6
994,182 + PS417_04495 0.47 -1.9
994,183 - PS417_04495 0.48 +0.9
994,183 - PS417_04495 0.48 -0.3
994,185 - PS417_04495 0.49 +0.3
994,185 - PS417_04495 0.49 +1.0
994,185 - PS417_04495 0.49 +1.2
994,185 - PS417_04495 0.49 +0.1
994,185 - PS417_04495 0.49 -3.0
994,185 - PS417_04495 0.49 +0.8
994,185 - PS417_04495 0.49 -2.7
994,185 - PS417_04495 0.49 +0.4
994,185 - PS417_04495 0.49 +0.7
994,185 - PS417_04495 0.49 +0.4
994,284 - +1.8
994,284 - -0.5
994,284 - -2.2
994,303 - +1.4
994,485 + PS417_04500 0.75 -0.3
994,485 + PS417_04500 0.75 -1.6
994,548 + +0.9
994,549 - -1.2
995,272 + -1.4
995,279 + +0.5
995,279 + -0.5
995,280 - -2.0
995,280 - -0.3
995,280 - +0.9
995,285 + -0.2
995,285 + -1.2
995,285 + +0.3
995,286 - -0.5
995,286 - -0.2
995,286 - +0.6
995,286 - -1.2
995,286 - -2.9

Or see this region's nucleotide sequence