Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04305 and PS417_04310 are separated by 27 nucleotides PS417_04310 and PS417_04315 are separated by 1 nucleotides PS417_04315 and PS417_04320 are separated by 33 nucleotides
PS417_04305: PS417_04305 - aspartate ammonia-lyase, at 965,591 to 966,967
_04305
PS417_04310: PS417_04310 - hypothetical protein, at 966,995 to 967,423
_04310
PS417_04315: PS417_04315 - superoxide dismutase, at 967,425 to 968,036
_04315
PS417_04320: PS417_04320 - divalent cation transporter, at 968,070 to 968,963
_04320
Position (kb) 966
967
968 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 966.225 kb on + strand, within PS417_04305 at 966.965 kb on + strand at 967.039 kb on + strand, within PS417_04310 at 967.039 kb on + strand, within PS417_04310 at 967.040 kb on - strand, within PS417_04310 at 967.073 kb on + strand, within PS417_04310 at 967.073 kb on + strand, within PS417_04310 at 967.074 kb on - strand, within PS417_04310 at 967.424 kb on + strand at 967.425 kb on - strand at 967.431 kb on + strand at 967.431 kb on + strand at 967.432 kb on - strand at 967.432 kb on - strand at 967.432 kb on - strand at 967.433 kb on + strand at 967.433 kb on + strand at 967.458 kb on + strand at 967.478 kb on + strand at 967.507 kb on - strand, within PS417_04315 at 967.507 kb on - strand, within PS417_04315 at 967.507 kb on - strand, within PS417_04315 at 967.527 kb on + strand, within PS417_04315 at 967.527 kb on + strand, within PS417_04315 at 967.935 kb on + strand, within PS417_04315 at 967.936 kb on - strand, within PS417_04315 at 967.936 kb on - strand, within PS417_04315 at 967.945 kb on - strand, within PS417_04315 at 967.966 kb on - strand, within PS417_04315 at 967.980 kb on + strand at 967.981 kb on - strand at 967.988 kb on + strand at 968.017 kb on - strand at 968.059 kb on + strand at 968.059 kb on + strand at 968.059 kb on + strand at 968.059 kb on + strand at 968.138 kb on - strand at 968.148 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 966,225 + PS417_04305 0.46 +1.1 966,965 + +4.6 967,039 + PS417_04310 0.10 -1.0 967,039 + PS417_04310 0.10 +2.2 967,040 - PS417_04310 0.10 +1.0 967,073 + PS417_04310 0.18 +1.1 967,073 + PS417_04310 0.18 +4.5 967,074 - PS417_04310 0.18 -0.1 967,424 + +0.8 967,425 - +1.0 967,431 + -0.4 967,431 + +0.1 967,432 - -0.5 967,432 - -0.6 967,432 - -0.2 967,433 + -0.8 967,433 + +0.5 967,458 + +0.4 967,478 + -0.2 967,507 - PS417_04315 0.13 +0.4 967,507 - PS417_04315 0.13 +0.2 967,507 - PS417_04315 0.13 -0.7 967,527 + PS417_04315 0.17 +0.4 967,527 + PS417_04315 0.17 -0.3 967,935 + PS417_04315 0.83 +0.3 967,936 - PS417_04315 0.83 -1.2 967,936 - PS417_04315 0.83 +1.4 967,945 - PS417_04315 0.85 -1.4 967,966 - PS417_04315 0.88 -2.9 967,980 + -2.5 967,981 - -1.4 967,988 + -1.2 968,017 - -0.2 968,059 + +3.5 968,059 + -1.0 968,059 + -0.2 968,059 + -1.3 968,138 - +0.0 968,148 - -0.2
Or see this region's nucleotide sequence