Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04215 and PS417_04220 overlap by 4 nucleotides PS417_04220 and PS417_04225 overlap by 4 nucleotides
PS417_04215: PS417_04215 - malate transporter, at 945,469 to 946,410
_04215
PS417_04220: PS417_04220 - 4-carboxymuconolactone decarboxylase, at 946,407 to 946,787
_04220
PS417_04225: PS417_04225 - conjugal transfer protein TraR, at 946,784 to 947,830
_04225
Position (kb) 946
947 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 945.434 kb on - strand at 945.441 kb on + strand at 945.682 kb on + strand, within PS417_04215 at 945.766 kb on + strand, within PS417_04215 at 945.766 kb on + strand, within PS417_04215 at 945.766 kb on + strand, within PS417_04215 at 945.767 kb on - strand, within PS417_04215 at 945.767 kb on - strand, within PS417_04215 at 945.767 kb on - strand, within PS417_04215 at 945.767 kb on - strand, within PS417_04215 at 945.767 kb on - strand, within PS417_04215 at 945.767 kb on - strand, within PS417_04215 at 945.768 kb on + strand, within PS417_04215 at 945.829 kb on + strand, within PS417_04215 at 945.886 kb on + strand, within PS417_04215 at 945.887 kb on - strand, within PS417_04215 at 945.920 kb on - strand, within PS417_04215 at 945.920 kb on - strand, within PS417_04215 at 946.252 kb on + strand, within PS417_04215 at 946.252 kb on + strand, within PS417_04215 at 946.253 kb on - strand, within PS417_04215 at 946.408 kb on + strand at 946.409 kb on - strand at 946.484 kb on - strand, within PS417_04220 at 946.866 kb on + strand at 946.866 kb on + strand at 946.866 kb on + strand at 946.866 kb on + strand at 946.866 kb on + strand at 946.867 kb on - strand at 946.869 kb on + strand at 946.869 kb on + strand at 946.869 kb on + strand at 946.869 kb on + strand at 946.870 kb on - strand at 946.870 kb on - strand at 946.870 kb on - strand at 946.870 kb on - strand at 946.915 kb on + strand, within PS417_04225 at 946.915 kb on + strand, within PS417_04225 at 947.620 kb on + strand, within PS417_04225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 945,434 - +1.0 945,441 + +0.8 945,682 + PS417_04215 0.23 +1.3 945,766 + PS417_04215 0.32 -0.2 945,766 + PS417_04215 0.32 -1.4 945,766 + PS417_04215 0.32 -3.2 945,767 - PS417_04215 0.32 +0.7 945,767 - PS417_04215 0.32 +0.7 945,767 - PS417_04215 0.32 +0.2 945,767 - PS417_04215 0.32 -0.5 945,767 - PS417_04215 0.32 -1.4 945,767 - PS417_04215 0.32 +0.1 945,768 + PS417_04215 0.32 +0.5 945,829 + PS417_04215 0.38 +1.8 945,886 + PS417_04215 0.44 -2.3 945,887 - PS417_04215 0.44 +0.2 945,920 - PS417_04215 0.48 -2.6 945,920 - PS417_04215 0.48 -0.4 946,252 + PS417_04215 0.83 +1.6 946,252 + PS417_04215 0.83 +0.9 946,253 - PS417_04215 0.83 -2.3 946,408 + -0.6 946,409 - +1.0 946,484 - PS417_04220 0.20 -1.2 946,866 + -1.6 946,866 + -3.0 946,866 + -0.8 946,866 + -1.6 946,866 + +1.1 946,867 - +1.8 946,869 + -3.5 946,869 + -1.8 946,869 + -3.4 946,869 + -1.6 946,870 - -1.4 946,870 - -0.4 946,870 - +0.2 946,870 - -0.5 946,915 + PS417_04225 0.13 -0.2 946,915 + PS417_04225 0.13 +0.3 947,620 + PS417_04225 0.80 +0.4
Or see this region's nucleotide sequence