Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04030 and PS417_04035 are separated by 167 nucleotides PS417_04035 and PS417_04040 are separated by 96 nucleotides PS417_04040 and PS417_04045 are separated by 113 nucleotides
PS417_04030: PS417_04030 - peptide chain release factor 3, at 904,777 to 906,360
_04030
PS417_04035: PS417_04035 - hypothetical protein, at 906,528 to 906,773
_04035
PS417_04040: PS417_04040 - hypothetical protein, at 906,870 to 907,124
_04040
PS417_04045: PS417_04045 - peptide ABC transporter ATP-binding protein, at 907,238 to 908,209
_04045
Position (kb)
906
907 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 906.376 kb on + strand at 906.376 kb on + strand at 906.377 kb on - strand at 906.484 kb on + strand at 906.484 kb on + strand at 906.485 kb on - strand at 906.492 kb on + strand at 906.533 kb on + strand at 906.534 kb on - strand at 906.534 kb on - strand at 906.534 kb on - strand at 906.534 kb on - strand at 906.726 kb on + strand, within PS417_04035 at 906.727 kb on - strand, within PS417_04035 at 906.753 kb on + strand at 906.754 kb on - strand at 906.844 kb on + strand at 906.844 kb on + strand at 906.844 kb on + strand at 907.122 kb on + strand at 907.123 kb on - strand at 907.186 kb on - strand at 907.186 kb on - strand at 907.590 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 906,376 + -0.9 906,376 + +0.8 906,377 - -1.7 906,484 + -2.3 906,484 + -3.7 906,485 - -1.1 906,492 + +1.0 906,533 + -1.2 906,534 - -2.1 906,534 - -0.6 906,534 - -1.0 906,534 - -0.4 906,726 + PS417_04035 0.80 +1.2 906,727 - PS417_04035 0.81 +0.2 906,753 + -0.4 906,754 - +1.6 906,844 + -0.0 906,844 + +0.2 906,844 + -0.9 907,122 + +0.7 907,123 - +0.2 907,186 - -0.5 907,186 - +0.3 907,590 + +2.1
Or see this region's nucleotide sequence