Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03705 and PS417_03710 are separated by 101 nucleotides PS417_03710 and PS417_03715 are separated by 150 nucleotides
PS417_03705: PS417_03705 - 2-alkenal reductase, at 838,705 to 839,859
_03705
PS417_03710: PS417_03710 - metal-binding protein, at 839,961 to 840,719
_03710
PS417_03715: PS417_03715 - sulfate adenylyltransferase subunit 2, at 840,870 to 841,787
_03715
Position (kb)
839
840
841 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 839.510 kb on + strand, within PS417_03705 at 839.652 kb on - strand, within PS417_03705 at 839.713 kb on + strand, within PS417_03705 at 839.713 kb on + strand, within PS417_03705 at 839.713 kb on + strand, within PS417_03705 at 839.916 kb on - strand at 839.916 kb on - strand at 839.918 kb on - strand at 839.918 kb on - strand at 839.918 kb on - strand at 839.918 kb on - strand at 839.949 kb on + strand at 839.950 kb on - strand at 839.950 kb on - strand at 839.961 kb on - strand at 840.162 kb on + strand, within PS417_03710 at 840.507 kb on + strand, within PS417_03710 at 840.507 kb on + strand, within PS417_03710 at 840.508 kb on - strand, within PS417_03710 at 840.543 kb on + strand, within PS417_03710 at 840.543 kb on + strand, within PS417_03710 at 840.544 kb on - strand, within PS417_03710 at 840.643 kb on + strand, within PS417_03710 at 840.643 kb on + strand, within PS417_03710 at 840.643 kb on + strand, within PS417_03710 at 840.643 kb on + strand, within PS417_03710 at 840.644 kb on - strand at 840.758 kb on + strand at 840.758 kb on + strand at 840.773 kb on + strand at 840.807 kb on + strand at 840.807 kb on + strand at 840.808 kb on - strand at 840.808 kb on - strand at 840.845 kb on + strand at 840.845 kb on + strand at 840.845 kb on + strand at 840.845 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 839,510 + PS417_03705 0.70 +1.3 839,652 - PS417_03705 0.82 -2.6 839,713 + PS417_03705 0.87 -3.8 839,713 + PS417_03705 0.87 -0.3 839,713 + PS417_03705 0.87 -2.0 839,916 - -1.7 839,916 - +0.5 839,918 - -1.9 839,918 - +0.2 839,918 - +1.2 839,918 - +0.3 839,949 + +0.5 839,950 - +0.2 839,950 - -0.5 839,961 - +0.9 840,162 + PS417_03710 0.26 -2.4 840,507 + PS417_03710 0.72 -0.7 840,507 + PS417_03710 0.72 -0.2 840,508 - PS417_03710 0.72 -1.0 840,543 + PS417_03710 0.77 -1.8 840,543 + PS417_03710 0.77 +0.4 840,544 - PS417_03710 0.77 -1.6 840,643 + PS417_03710 0.90 +0.3 840,643 + PS417_03710 0.90 +0.6 840,643 + PS417_03710 0.90 -0.5 840,643 + PS417_03710 0.90 -0.8 840,644 - +1.2 840,758 + +0.7 840,758 + +0.4 840,773 + -0.4 840,807 + +1.1 840,807 + +0.6 840,808 - +1.3 840,808 - +0.2 840,845 + -1.1 840,845 + +0.5 840,845 + +0.5 840,845 + -0.2
Or see this region's nucleotide sequence