Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00345 and PS417_00350 are separated by 161 nucleotides PS417_00350 and PS417_00355 are separated by 51 nucleotides PS417_00355 and PS417_00360 overlap by 1 nucleotides
PS417_00345: PS417_00345 - copper-binding protein, at 66,901 to 67,536
_00345
PS417_00350: PS417_00350 - methionine ABC transporter substrate-binding protein, at 67,698 to 68,468
_00350
PS417_00355: PS417_00355 - metal ABC transporter permease, at 68,520 to 69,194
_00355
PS417_00360: PS417_00360 - methionine ABC transporter ATP-binding protein, at 69,194 to 70,201
_00360
Position (kb) 68
69
70 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.535 kb on - strand at 67.727 kb on - strand at 67.834 kb on + strand, within PS417_00350 at 67.835 kb on - strand, within PS417_00350 at 68.146 kb on + strand, within PS417_00350 at 68.146 kb on + strand, within PS417_00350 at 68.286 kb on + strand, within PS417_00350 at 68.286 kb on + strand, within PS417_00350 at 68.695 kb on + strand, within PS417_00355 at 68.696 kb on - strand, within PS417_00355 at 68.696 kb on - strand, within PS417_00355 at 68.709 kb on - strand, within PS417_00355 at 69.090 kb on + strand, within PS417_00355 at 69.196 kb on - strand at 70.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 67,530 + +0.8 67,530 + -2.7 67,530 + +0.0 67,531 - -0.1 67,531 - +0.3 67,535 - +0.0 67,727 - -2.2 67,834 + PS417_00350 0.18 +0.2 67,835 - PS417_00350 0.18 +1.8 68,146 + PS417_00350 0.58 -0.9 68,146 + PS417_00350 0.58 -4.3 68,286 + PS417_00350 0.76 +0.1 68,286 + PS417_00350 0.76 +1.0 68,695 + PS417_00355 0.26 -0.5 68,696 - PS417_00355 0.26 -2.4 68,696 - PS417_00355 0.26 -2.5 68,709 - PS417_00355 0.28 -1.2 69,090 + PS417_00355 0.84 -2.9 69,196 - -2.4 70,178 - -2.6
Or see this region's nucleotide sequence