Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03185 and PS417_03190 are separated by 144 nucleotides PS417_03190 and PS417_03195 are separated by 118 nucleotides PS417_03195 and PS417_03200 are separated by 137 nucleotides PS417_03200 and PS417_03205 are separated by 24 nucleotides
PS417_03185: PS417_03185 - NADPH:quinone reductase, at 729,319 to 730,332
_03185
PS417_03190: PS417_03190 - antibiotic biosynthesis monooxygenase, at 730,477 to 730,770
_03190
PS417_03195: PS417_03195 - LysR family transcriptional regulator, at 730,889 to 731,815
_03195
PS417_03200: PS417_03200 - ArsR family transcriptional regulator, at 731,953 to 732,642
_03200
PS417_03205: PS417_03205 - adenine deaminase, at 732,667 to 733,620
_03205
Position (kb) 730
731
732 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 729.996 kb on + strand, within PS417_03185 at 729.997 kb on - strand, within PS417_03185 at 729.997 kb on - strand, within PS417_03185 at 730.024 kb on - strand, within PS417_03185 at 730.068 kb on - strand, within PS417_03185 at 730.148 kb on - strand, within PS417_03185 at 730.148 kb on - strand, within PS417_03185 at 730.148 kb on - strand, within PS417_03185 at 730.313 kb on + strand at 730.313 kb on + strand at 730.314 kb on - strand at 730.314 kb on - strand at 730.314 kb on - strand at 730.314 kb on - strand at 730.317 kb on - strand at 730.534 kb on - strand, within PS417_03190 at 730.610 kb on + strand, within PS417_03190 at 730.649 kb on + strand, within PS417_03190 at 730.650 kb on - strand, within PS417_03190 at 730.770 kb on + strand at 730.804 kb on + strand at 730.804 kb on + strand at 730.805 kb on - strand at 730.805 kb on - strand at 730.817 kb on - strand at 730.832 kb on - strand at 730.920 kb on + strand at 730.921 kb on - strand at 730.921 kb on - strand at 730.921 kb on - strand at 731.024 kb on - strand, within PS417_03195 at 731.465 kb on + strand, within PS417_03195 at 731.465 kb on + strand, within PS417_03195 at 731.465 kb on + strand, within PS417_03195 at 731.465 kb on + strand, within PS417_03195 at 731.465 kb on + strand, within PS417_03195 at 731.465 kb on + strand, within PS417_03195 at 731.465 kb on + strand, within PS417_03195 at 731.466 kb on - strand, within PS417_03195 at 731.466 kb on - strand, within PS417_03195 at 731.466 kb on - strand, within PS417_03195 at 731.466 kb on - strand, within PS417_03195 at 731.467 kb on + strand, within PS417_03195 at 731.468 kb on - strand, within PS417_03195 at 731.468 kb on - strand, within PS417_03195 at 731.468 kb on - strand, within PS417_03195 at 731.606 kb on + strand, within PS417_03195 at 731.607 kb on - strand, within PS417_03195 at 731.813 kb on + strand at 731.863 kb on - strand at 731.902 kb on - strand at 731.903 kb on + strand at 731.904 kb on - strand at 731.904 kb on - strand at 731.916 kb on - strand at 732.288 kb on - strand, within PS417_03200 at 732.344 kb on - strand, within PS417_03200 at 732.471 kb on + strand, within PS417_03200 at 732.472 kb on - strand, within PS417_03200 at 732.472 kb on - strand, within PS417_03200 at 732.472 kb on - strand at 732.645 kb on + strand at 732.645 kb on + strand at 732.646 kb on - strand at 732.646 kb on - strand at 732.646 kb on - strand at 732.669 kb on - strand at 732.677 kb on - strand at 732.773 kb on + strand, within PS417_03205
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 729,996 + PS417_03185 0.67 -0.6 729,997 - PS417_03185 0.67 +1.2 729,997 - PS417_03185 0.67 -1.4 730,024 - PS417_03185 0.70 -2.7 730,068 - PS417_03185 0.74 -0.6 730,148 - PS417_03185 0.82 -0.5 730,148 - PS417_03185 0.82 +1.8 730,148 - PS417_03185 0.82 -2.0 730,313 + -0.4 730,313 + +0.3 730,314 - +0.2 730,314 - -0.5 730,314 - +0.2 730,314 - +1.0 730,317 - +0.0 730,534 - PS417_03190 0.19 +0.0 730,610 + PS417_03190 0.45 -0.4 730,649 + PS417_03190 0.59 +0.4 730,650 - PS417_03190 0.59 -2.1 730,770 + -0.5 730,804 + -1.0 730,804 + -1.9 730,805 - -0.3 730,805 - +0.4 730,817 - -0.4 730,832 - -1.5 730,920 + -0.2 730,921 - -2.1 730,921 - -1.7 730,921 - -1.0 731,024 - PS417_03195 0.15 -0.1 731,465 + PS417_03195 0.62 -1.0 731,465 + PS417_03195 0.62 -1.5 731,465 + PS417_03195 0.62 -3.0 731,465 + PS417_03195 0.62 +0.1 731,465 + PS417_03195 0.62 +3.2 731,465 + PS417_03195 0.62 +1.3 731,465 + PS417_03195 0.62 +0.9 731,466 - PS417_03195 0.62 -2.4 731,466 - PS417_03195 0.62 +0.7 731,466 - PS417_03195 0.62 +0.2 731,466 - PS417_03195 0.62 -1.4 731,467 + PS417_03195 0.62 +0.2 731,468 - PS417_03195 0.62 +0.2 731,468 - PS417_03195 0.62 -0.0 731,468 - PS417_03195 0.62 +1.1 731,606 + PS417_03195 0.77 +3.3 731,607 - PS417_03195 0.77 +0.1 731,813 + +0.3 731,863 - +1.2 731,902 - +0.1 731,903 + +0.3 731,904 - +1.3 731,904 - +1.6 731,916 - +1.4 732,288 - PS417_03200 0.49 +0.7 732,344 - PS417_03200 0.57 +1.3 732,471 + PS417_03200 0.75 -0.5 732,472 - PS417_03200 0.75 -2.1 732,472 - PS417_03200 0.75 +1.5 732,472 - +1.6 732,645 + -0.5 732,645 + +1.1 732,646 - -0.2 732,646 - +0.4 732,646 - +0.0 732,669 - +0.2 732,677 - -0.1 732,773 + PS417_03205 0.11 -0.5
Or see this region's nucleotide sequence