Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03125 and PS417_03130 are separated by 172 nucleotides PS417_03130 and PS417_03135 are separated by 301 nucleotides
PS417_03125: PS417_03125 - potassium ABC transporter ATPase, at 715,758 to 717,440
_03125
PS417_03130: PS417_03130 - hypothetical protein, at 717,613 to 719,256
_03130
PS417_03135: PS417_03135 - acyl dehydratase, at 719,558 to 720,406
_03135
Position (kb) 717
718
719
720 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 716.635 kb on + strand, within PS417_03125 at 716.648 kb on + strand, within PS417_03125 at 716.649 kb on - strand, within PS417_03125 at 716.678 kb on + strand, within PS417_03125 at 716.992 kb on + strand, within PS417_03125 at 717.002 kb on + strand, within PS417_03125 at 717.003 kb on - strand, within PS417_03125 at 717.004 kb on + strand, within PS417_03125 at 717.004 kb on + strand, within PS417_03125 at 717.005 kb on - strand, within PS417_03125 at 717.080 kb on - strand, within PS417_03125 at 717.166 kb on + strand, within PS417_03125 at 717.397 kb on + strand at 717.417 kb on + strand at 717.440 kb on + strand at 717.440 kb on + strand at 717.544 kb on - strand at 717.544 kb on - strand at 717.572 kb on - strand at 717.641 kb on - strand at 717.772 kb on + strand at 718.116 kb on + strand, within PS417_03130 at 718.117 kb on - strand, within PS417_03130 at 718.264 kb on + strand, within PS417_03130 at 718.265 kb on - strand, within PS417_03130 at 718.265 kb on - strand, within PS417_03130 at 718.456 kb on - strand, within PS417_03130 at 718.500 kb on + strand, within PS417_03130 at 718.528 kb on + strand, within PS417_03130 at 718.528 kb on + strand, within PS417_03130 at 718.529 kb on - strand, within PS417_03130 at 718.966 kb on - strand, within PS417_03130 at 718.966 kb on - strand, within PS417_03130 at 718.966 kb on - strand, within PS417_03130 at 719.038 kb on - strand, within PS417_03130 at 719.161 kb on - strand at 719.204 kb on - strand at 719.223 kb on + strand at 719.242 kb on + strand at 719.243 kb on - strand at 719.277 kb on + strand at 719.345 kb on - strand at 719.378 kb on - strand at 719.385 kb on - strand at 719.396 kb on - strand at 719.534 kb on - strand at 719.535 kb on + strand at 719.535 kb on + strand at 719.535 kb on + strand at 719.537 kb on + strand at 719.539 kb on + strand at 719.587 kb on + strand at 719.657 kb on + strand, within PS417_03135 at 719.657 kb on + strand, within PS417_03135 at 719.658 kb on - strand, within PS417_03135 at 719.987 kb on + strand, within PS417_03135 at 719.987 kb on + strand, within PS417_03135 at 719.987 kb on + strand, within PS417_03135 at 720.213 kb on + strand, within PS417_03135 at 720.214 kb on - strand, within PS417_03135 at 720.214 kb on - strand, within PS417_03135
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 716,635 + PS417_03125 0.52 -0.4 716,648 + PS417_03125 0.53 +1.5 716,649 - PS417_03125 0.53 -2.5 716,678 + PS417_03125 0.55 +0.3 716,992 + PS417_03125 0.73 -2.1 717,002 + PS417_03125 0.74 +2.3 717,003 - PS417_03125 0.74 -0.5 717,004 + PS417_03125 0.74 +0.9 717,004 + PS417_03125 0.74 +1.3 717,005 - PS417_03125 0.74 +1.0 717,080 - PS417_03125 0.79 -0.3 717,166 + PS417_03125 0.84 +0.2 717,397 + +0.2 717,417 + -1.2 717,440 + +0.8 717,440 + +2.6 717,544 - +0.8 717,544 - -2.4 717,572 - -1.7 717,641 - -0.3 717,772 + -0.8 718,116 + PS417_03130 0.31 -0.2 718,117 - PS417_03130 0.31 +3.0 718,264 + PS417_03130 0.40 -2.8 718,265 - PS417_03130 0.40 -0.4 718,265 - PS417_03130 0.40 -2.6 718,456 - PS417_03130 0.51 -0.7 718,500 + PS417_03130 0.54 -1.2 718,528 + PS417_03130 0.56 -1.2 718,528 + PS417_03130 0.56 -2.2 718,529 - PS417_03130 0.56 -0.8 718,966 - PS417_03130 0.82 +0.2 718,966 - PS417_03130 0.82 -0.6 718,966 - PS417_03130 0.82 +0.8 719,038 - PS417_03130 0.87 -1.0 719,161 - -0.2 719,204 - -0.3 719,223 + +0.7 719,242 + -1.2 719,243 - -1.2 719,277 + +0.3 719,345 - +0.7 719,378 - -1.6 719,385 - +0.9 719,396 - +0.8 719,534 - +1.4 719,535 + +1.2 719,535 + +0.3 719,535 + -0.8 719,537 + -1.9 719,539 + -1.6 719,587 + +2.6 719,657 + PS417_03135 0.12 +1.4 719,657 + PS417_03135 0.12 +0.4 719,658 - PS417_03135 0.12 +0.4 719,987 + PS417_03135 0.51 +0.4 719,987 + PS417_03135 0.51 -2.4 719,987 + PS417_03135 0.51 +0.0 720,213 + PS417_03135 0.77 +0.1 720,214 - PS417_03135 0.77 -1.4 720,214 - PS417_03135 0.77 +0.5
Or see this region's nucleotide sequence