Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03015 and PS417_03020 are separated by 99 nucleotides PS417_03020 and PS417_03025 are separated by 251 nucleotides PS417_03025 and PS417_03030 are separated by 151 nucleotides PS417_03030 and PS417_03035 overlap by 8 nucleotides
PS417_03015: PS417_03015 - hypothetical protein, at 690,357 to 695,255
_03015
PS417_03020: PS417_03020 - MFS transporter, at 695,355 to 695,648
_03020
PS417_03025: PS417_03025 - penicillin-binding protein 1C, at 695,900 to 698,191
_03025
PS417_03030: PS417_03030 - pyrophosphatase, at 698,343 to 698,651
_03030
PS417_03035: PS417_03035 - SAM-dependent methyltransferase, at 698,644 to 699,393
_03035
Position (kb) 695
696
697
698
699 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 695.083 kb on - strand at 695.083 kb on - strand at 695.083 kb on - strand at 695.257 kb on - strand at 695.279 kb on - strand at 695.335 kb on - strand at 695.335 kb on - strand at 695.335 kb on - strand at 695.346 kb on - strand at 695.346 kb on - strand at 695.588 kb on + strand, within PS417_03020 at 695.648 kb on + strand at 695.648 kb on + strand at 695.648 kb on + strand at 695.648 kb on + strand at 695.648 kb on + strand at 695.648 kb on + strand at 695.648 kb on + strand at 695.648 kb on + strand at 695.648 kb on + strand at 695.648 kb on + strand at 695.649 kb on - strand at 695.649 kb on - strand at 695.649 kb on - strand at 695.649 kb on - strand at 695.649 kb on - strand at 695.649 kb on - strand at 695.649 kb on - strand at 695.649 kb on - strand at 695.708 kb on + strand at 695.708 kb on + strand at 695.708 kb on + strand at 695.752 kb on + strand at 695.817 kb on + strand at 695.877 kb on + strand at 695.878 kb on - strand at 696.072 kb on - strand at 696.072 kb on - strand at 696.075 kb on - strand at 696.095 kb on + strand at 696.095 kb on + strand at 696.096 kb on - strand at 696.113 kb on + strand at 696.327 kb on - strand, within PS417_03025 at 696.742 kb on + strand, within PS417_03025 at 696.743 kb on - strand, within PS417_03025 at 696.743 kb on - strand, within PS417_03025 at 696.743 kb on - strand at 696.840 kb on + strand, within PS417_03025 at 696.840 kb on + strand, within PS417_03025 at 696.840 kb on + strand, within PS417_03025 at 696.840 kb on + strand, within PS417_03025 at 696.840 kb on + strand, within PS417_03025 at 696.840 kb on + strand, within PS417_03025 at 696.841 kb on - strand, within PS417_03025 at 696.841 kb on - strand, within PS417_03025 at 696.841 kb on - strand, within PS417_03025 at 696.841 kb on - strand, within PS417_03025 at 697.202 kb on + strand, within PS417_03025 at 697.279 kb on - strand, within PS417_03025 at 698.394 kb on - strand, within PS417_03030 at 698.556 kb on + strand, within PS417_03030 at 698.556 kb on + strand, within PS417_03030 at 698.556 kb on + strand, within PS417_03030 at 698.557 kb on - strand, within PS417_03030 at 698.805 kb on + strand, within PS417_03035 at 698.805 kb on + strand, within PS417_03035 at 698.805 kb on + strand, within PS417_03035 at 698.806 kb on - strand, within PS417_03035 at 698.806 kb on - strand, within PS417_03035 at 698.806 kb on - strand, within PS417_03035 at 698.806 kb on - strand, within PS417_03035 at 698.806 kb on - strand, within PS417_03035 at 698.806 kb on - strand, within PS417_03035 at 698.919 kb on + strand, within PS417_03035 at 698.920 kb on - strand, within PS417_03035 at 698.965 kb on + strand, within PS417_03035 at 699.168 kb on + strand, within PS417_03035 at 699.169 kb on - strand, within PS417_03035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 695,083 - +1.8 695,083 - -2.5 695,083 - -0.3 695,257 - +0.3 695,279 - +0.2 695,335 - +0.8 695,335 - -1.0 695,335 - +1.8 695,346 - -1.8 695,346 - +0.8 695,588 + PS417_03020 0.79 +2.0 695,648 + -1.4 695,648 + +1.1 695,648 + +0.5 695,648 + -0.5 695,648 + +0.5 695,648 + +2.6 695,648 + -0.8 695,648 + +0.1 695,648 + -0.6 695,648 + +0.5 695,649 - +0.2 695,649 - -1.9 695,649 - -0.5 695,649 - -0.2 695,649 - +1.8 695,649 - -0.9 695,649 - +0.2 695,649 - -0.7 695,708 + -2.6 695,708 + +0.2 695,708 + +0.3 695,752 + -0.5 695,817 + -0.1 695,877 + +0.9 695,878 - +0.6 696,072 - -1.4 696,072 - +0.6 696,075 - -0.9 696,095 + +0.2 696,095 + -1.8 696,096 - -0.6 696,113 + -0.5 696,327 - PS417_03025 0.19 +0.3 696,742 + PS417_03025 0.37 -0.5 696,743 - PS417_03025 0.37 +0.3 696,743 - PS417_03025 0.37 -0.2 696,743 - -1.0 696,840 + PS417_03025 0.41 +0.2 696,840 + PS417_03025 0.41 +2.5 696,840 + PS417_03025 0.41 +0.8 696,840 + PS417_03025 0.41 +0.7 696,840 + PS417_03025 0.41 -1.2 696,840 + PS417_03025 0.41 -1.4 696,841 - PS417_03025 0.41 +1.0 696,841 - PS417_03025 0.41 +0.3 696,841 - PS417_03025 0.41 +1.0 696,841 - PS417_03025 0.41 +1.1 697,202 + PS417_03025 0.57 +0.2 697,279 - PS417_03025 0.60 +2.8 698,394 - PS417_03030 0.17 +0.6 698,556 + PS417_03030 0.69 -0.1 698,556 + PS417_03030 0.69 -2.9 698,556 + PS417_03030 0.69 -3.4 698,557 - PS417_03030 0.69 +0.5 698,805 + PS417_03035 0.21 +0.4 698,805 + PS417_03035 0.21 -2.0 698,805 + PS417_03035 0.21 -0.8 698,806 - PS417_03035 0.22 -0.6 698,806 - PS417_03035 0.22 +1.8 698,806 - PS417_03035 0.22 +0.3 698,806 - PS417_03035 0.22 +1.1 698,806 - PS417_03035 0.22 +1.5 698,806 - PS417_03035 0.22 -0.1 698,919 + PS417_03035 0.37 -0.1 698,920 - PS417_03035 0.37 +0.3 698,965 + PS417_03035 0.43 -1.0 699,168 + PS417_03035 0.70 -0.2 699,169 - PS417_03035 0.70 -0.2
Or see this region's nucleotide sequence