Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02875 and PS417_02880 are separated by 11 nucleotides PS417_02880 and PS417_02885 are separated by 47 nucleotides PS417_02885 and PS417_02890 are separated by 262 nucleotides PS417_02890 and PS417_02895 overlap by 4 nucleotides
PS417_02875: PS417_02875 - hypothetical protein, at 658,262 to 658,651
_02875
PS417_02880: PS417_02880 - copper chaperone, at 658,663 to 659,145
_02880
PS417_02885: PS417_02885 - hypothetical protein, at 659,193 to 659,588
_02885
PS417_02890: PS417_02890 - cobalt-precorrin-6X reductase, at 659,851 to 660,570
_02890
PS417_02895: PS417_02895 - precorrin-6Y C5,15-methyltransferase, at 660,567 to 661,772
_02895
Position (kb) 659
660 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 658.246 kb on + strand at 658.247 kb on - strand at 658.517 kb on - strand, within PS417_02875 at 658.664 kb on + strand at 658.877 kb on - strand, within PS417_02880 at 659.076 kb on - strand, within PS417_02880 at 659.285 kb on + strand, within PS417_02885 at 659.303 kb on - strand, within PS417_02885 at 659.303 kb on - strand, within PS417_02885 at 659.303 kb on - strand, within PS417_02885 at 659.470 kb on - strand, within PS417_02885 at 659.629 kb on - strand at 659.651 kb on - strand at 659.651 kb on - strand at 659.651 kb on - strand at 659.743 kb on - strand at 659.743 kb on - strand at 659.743 kb on - strand at 660.494 kb on + strand, within PS417_02890 at 660.494 kb on + strand, within PS417_02890 at 660.494 kb on + strand, within PS417_02890 at 660.498 kb on + strand, within PS417_02890 at 660.498 kb on + strand, within PS417_02890 at 660.498 kb on + strand, within PS417_02890 at 660.499 kb on - strand at 660.499 kb on - strand at 660.517 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 658,246 + +0.2 658,247 - +1.9 658,517 - PS417_02875 0.65 -4.0 658,664 + +0.8 658,877 - PS417_02880 0.44 +0.8 659,076 - PS417_02880 0.86 -3.7 659,285 + PS417_02885 0.23 +1.4 659,303 - PS417_02885 0.28 -1.8 659,303 - PS417_02885 0.28 -2.2 659,303 - PS417_02885 0.28 -0.4 659,470 - PS417_02885 0.70 -2.9 659,629 - -3.1 659,651 - -2.9 659,651 - -0.8 659,651 - +0.1 659,743 - -0.8 659,743 - -1.0 659,743 - -1.2 660,494 + PS417_02890 0.89 +1.8 660,494 + PS417_02890 0.89 +0.7 660,494 + PS417_02890 0.89 +0.7 660,498 + PS417_02890 0.90 -2.0 660,498 + PS417_02890 0.90 +0.2 660,498 + PS417_02890 0.90 -1.5 660,499 - -1.3 660,499 - +0.4 660,517 - -2.4
Or see this region's nucleotide sequence