Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02845 and PS417_02850 overlap by 4 nucleotides PS417_02850 and PS417_02855 overlap by 4 nucleotides PS417_02855 and PS417_02860 are separated by 23 nucleotides
PS417_02845: PS417_02845 - ABC transporter permease, at 649,583 to 650,593
_02845
PS417_02850: PS417_02850 - iron ABC transporter substrate-binding protein, at 650,590 to 651,537
_02850
PS417_02855: PS417_02855 - histidinol phosphatase, at 651,534 to 652,316
_02855
PS417_02860: PS417_02860 - TonB-dependent receptor, at 652,340 to 654,448
_02860
Position (kb) 650
651
652 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 649.607 kb on - strand at 650.182 kb on + strand at 650.417 kb on - strand, within PS417_02845 at 650.466 kb on + strand, within PS417_02845 at 650.466 kb on + strand, within PS417_02845 at 650.467 kb on - strand, within PS417_02845 at 650.568 kb on - strand at 650.570 kb on + strand at 650.570 kb on + strand at 650.570 kb on + strand at 650.663 kb on + strand at 650.677 kb on - strand at 650.839 kb on + strand, within PS417_02850 at 650.839 kb on + strand, within PS417_02850 at 650.839 kb on + strand, within PS417_02850 at 650.839 kb on + strand, within PS417_02850 at 650.839 kb on + strand, within PS417_02850 at 650.840 kb on - strand, within PS417_02850 at 650.840 kb on - strand, within PS417_02850 at 650.840 kb on - strand, within PS417_02850 at 650.840 kb on - strand, within PS417_02850 at 650.853 kb on + strand, within PS417_02850 at 650.936 kb on + strand, within PS417_02850 at 651.015 kb on + strand, within PS417_02850 at 651.066 kb on + strand, within PS417_02850 at 651.067 kb on - strand, within PS417_02850 at 651.068 kb on + strand, within PS417_02850 at 651.068 kb on + strand, within PS417_02850 at 651.068 kb on + strand, within PS417_02850 at 651.068 kb on + strand, within PS417_02850 at 651.068 kb on + strand, within PS417_02850 at 651.069 kb on - strand, within PS417_02850 at 651.069 kb on - strand, within PS417_02850 at 651.131 kb on + strand, within PS417_02850 at 651.254 kb on + strand, within PS417_02850 at 651.254 kb on + strand, within PS417_02850 at 651.464 kb on + strand at 651.554 kb on + strand at 651.641 kb on + strand, within PS417_02855 at 651.641 kb on + strand, within PS417_02855 at 651.678 kb on + strand, within PS417_02855 at 651.785 kb on - strand, within PS417_02855 at 651.969 kb on - strand, within PS417_02855 at 652.145 kb on + strand, within PS417_02855 at 652.145 kb on + strand, within PS417_02855 at 652.350 kb on + strand at 652.351 kb on - strand at 652.351 kb on - strand at 652.410 kb on + strand at 652.410 kb on + strand at 652.410 kb on + strand at 652.411 kb on - strand at 652.425 kb on + strand at 652.425 kb on + strand at 652.426 kb on - strand at 652.512 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 649,607 - +0.9 650,182 + -1.0 650,417 - PS417_02845 0.82 -1.6 650,466 + PS417_02845 0.87 -1.4 650,466 + PS417_02845 0.87 +0.4 650,467 - PS417_02845 0.87 +0.0 650,568 - -0.3 650,570 + +0.0 650,570 + +1.8 650,570 + +0.3 650,663 + +2.3 650,677 - -2.0 650,839 + PS417_02850 0.26 -1.4 650,839 + PS417_02850 0.26 -2.0 650,839 + PS417_02850 0.26 +0.3 650,839 + PS417_02850 0.26 -0.7 650,839 + PS417_02850 0.26 +1.5 650,840 - PS417_02850 0.26 +3.0 650,840 - PS417_02850 0.26 -0.8 650,840 - PS417_02850 0.26 -0.1 650,840 - PS417_02850 0.26 +2.4 650,853 + PS417_02850 0.28 -0.3 650,936 + PS417_02850 0.36 -3.1 651,015 + PS417_02850 0.45 -0.0 651,066 + PS417_02850 0.50 -0.4 651,067 - PS417_02850 0.50 +0.5 651,068 + PS417_02850 0.50 +0.0 651,068 + PS417_02850 0.50 -0.4 651,068 + PS417_02850 0.50 +2.4 651,068 + PS417_02850 0.50 +0.8 651,068 + PS417_02850 0.50 +0.1 651,069 - PS417_02850 0.51 +0.5 651,069 - PS417_02850 0.51 +0.2 651,131 + PS417_02850 0.57 -3.2 651,254 + PS417_02850 0.70 +1.9 651,254 + PS417_02850 0.70 +0.2 651,464 + +0.0 651,554 + +0.5 651,641 + PS417_02855 0.14 -0.0 651,641 + PS417_02855 0.14 -0.2 651,678 + PS417_02855 0.18 +0.6 651,785 - PS417_02855 0.32 +2.7 651,969 - PS417_02855 0.56 -0.5 652,145 + PS417_02855 0.78 -0.1 652,145 + PS417_02855 0.78 -0.5 652,350 + -0.2 652,351 - +2.2 652,351 - +1.0 652,410 + +1.6 652,410 + -2.1 652,410 + -0.9 652,411 - -1.2 652,425 + -0.7 652,425 + -0.5 652,426 - +2.5 652,512 + -2.4
Or see this region's nucleotide sequence