Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28375 and PS417_28380 overlap by 4 nucleotides PS417_28380 and PS417_28385 are separated by 12 nucleotides PS417_28385 and PS417_28390 are separated by 185 nucleotides PS417_28390 and PS417_28395 are separated by 10 nucleotides
PS417_28375: PS417_28375 - membrane protein, at 6,124,641 to 6,125,675
_28375
PS417_28380: PS417_28380 - protease, at 6,125,672 to 6,126,658
_28380
PS417_28385: PS417_28385 - membrane protein, at 6,126,671 to 6,128,575
_28385
PS417_28390: PS417_28390 - CDP-alcohol phosphatidyltransferase, at 6,128,761 to 6,129,384
_28390
PS417_28395: PS417_28395 - hypothetical protein, at 6,129,395 to 6,131,143
_28395
Position (kb)
6126
6127
6128
6129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6125.796 kb on + strand, within PS417_28380 at 6125.796 kb on + strand, within PS417_28380 at 6125.796 kb on + strand, within PS417_28380 at 6125.797 kb on - strand, within PS417_28380 at 6125.797 kb on - strand, within PS417_28380 at 6125.817 kb on + strand, within PS417_28380 at 6125.829 kb on + strand, within PS417_28380 at 6126.075 kb on + strand, within PS417_28380 at 6126.075 kb on + strand, within PS417_28380 at 6126.076 kb on - strand, within PS417_28380 at 6126.079 kb on + strand, within PS417_28380 at 6126.079 kb on + strand, within PS417_28380 at 6126.080 kb on - strand, within PS417_28380 at 6126.080 kb on - strand, within PS417_28380 at 6126.080 kb on - strand, within PS417_28380 at 6126.312 kb on + strand, within PS417_28380 at 6126.313 kb on - strand, within PS417_28380 at 6126.313 kb on - strand, within PS417_28380 at 6126.563 kb on - strand at 6126.716 kb on - strand at 6126.725 kb on + strand at 6126.725 kb on + strand at 6126.725 kb on + strand at 6126.772 kb on + strand at 6126.773 kb on - strand at 6126.773 kb on - strand at 6126.816 kb on + strand at 6127.045 kb on - strand, within PS417_28385 at 6127.045 kb on - strand, within PS417_28385 at 6127.045 kb on - strand, within PS417_28385 at 6127.256 kb on + strand, within PS417_28385 at 6127.270 kb on + strand, within PS417_28385 at 6127.271 kb on - strand, within PS417_28385 at 6127.272 kb on + strand, within PS417_28385 at 6127.272 kb on + strand, within PS417_28385 at 6127.272 kb on + strand, within PS417_28385 at 6127.272 kb on + strand, within PS417_28385 at 6127.273 kb on - strand, within PS417_28385 at 6127.273 kb on - strand, within PS417_28385 at 6127.273 kb on - strand, within PS417_28385 at 6127.273 kb on - strand, within PS417_28385 at 6127.273 kb on - strand, within PS417_28385 at 6127.505 kb on + strand, within PS417_28385 at 6127.602 kb on + strand, within PS417_28385 at 6127.701 kb on + strand, within PS417_28385 at 6127.701 kb on + strand, within PS417_28385 at 6127.701 kb on + strand, within PS417_28385 at 6127.702 kb on - strand, within PS417_28385 at 6127.702 kb on - strand, within PS417_28385 at 6127.702 kb on - strand, within PS417_28385 at 6127.705 kb on + strand, within PS417_28385 at 6127.705 kb on + strand, within PS417_28385 at 6127.705 kb on + strand, within PS417_28385 at 6127.706 kb on - strand, within PS417_28385 at 6127.874 kb on + strand, within PS417_28385 at 6127.874 kb on + strand, within PS417_28385 at 6127.875 kb on - strand, within PS417_28385 at 6127.994 kb on - strand, within PS417_28385 at 6128.386 kb on + strand at 6128.386 kb on + strand at 6128.387 kb on - strand at 6128.602 kb on - strand at 6128.641 kb on + strand at 6128.642 kb on - strand at 6128.642 kb on - strand at 6128.658 kb on + strand at 6128.659 kb on - strand at 6128.659 kb on - strand at 6128.773 kb on + strand at 6128.774 kb on - strand at 6129.029 kb on - strand, within PS417_28390 at 6129.228 kb on - strand, within PS417_28390 at 6129.320 kb on - strand, within PS417_28390 at 6129.320 kb on - strand, within PS417_28390
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 6,125,796 + PS417_28380 0.13 +0.7 6,125,796 + PS417_28380 0.13 -0.0 6,125,796 + PS417_28380 0.13 -0.1 6,125,797 - PS417_28380 0.13 -0.0 6,125,797 - PS417_28380 0.13 -1.5 6,125,817 + PS417_28380 0.15 +1.5 6,125,829 + PS417_28380 0.16 -1.3 6,126,075 + PS417_28380 0.41 +1.8 6,126,075 + PS417_28380 0.41 -0.1 6,126,076 - PS417_28380 0.41 -1.5 6,126,079 + PS417_28380 0.41 -1.9 6,126,079 + PS417_28380 0.41 +0.9 6,126,080 - PS417_28380 0.41 +0.4 6,126,080 - PS417_28380 0.41 -2.9 6,126,080 - PS417_28380 0.41 +0.8 6,126,312 + PS417_28380 0.65 -2.8 6,126,313 - PS417_28380 0.65 +0.1 6,126,313 - PS417_28380 0.65 +1.2 6,126,563 - -0.4 6,126,716 - -0.3 6,126,725 + +0.5 6,126,725 + -1.8 6,126,725 + -1.3 6,126,772 + -0.4 6,126,773 - -1.4 6,126,773 - +1.1 6,126,816 + -1.4 6,127,045 - PS417_28385 0.20 -0.5 6,127,045 - PS417_28385 0.20 +0.4 6,127,045 - PS417_28385 0.20 -0.1 6,127,256 + PS417_28385 0.31 -0.9 6,127,270 + PS417_28385 0.31 +0.7 6,127,271 - PS417_28385 0.31 -1.3 6,127,272 + PS417_28385 0.32 -1.1 6,127,272 + PS417_28385 0.32 -0.2 6,127,272 + PS417_28385 0.32 +0.0 6,127,272 + PS417_28385 0.32 -1.3 6,127,273 - PS417_28385 0.32 +0.4 6,127,273 - PS417_28385 0.32 -0.1 6,127,273 - PS417_28385 0.32 +0.7 6,127,273 - PS417_28385 0.32 +0.3 6,127,273 - PS417_28385 0.32 +0.7 6,127,505 + PS417_28385 0.44 -0.7 6,127,602 + PS417_28385 0.49 -0.1 6,127,701 + PS417_28385 0.54 -1.6 6,127,701 + PS417_28385 0.54 -0.3 6,127,701 + PS417_28385 0.54 -0.8 6,127,702 - PS417_28385 0.54 -0.6 6,127,702 - PS417_28385 0.54 +0.3 6,127,702 - PS417_28385 0.54 +0.0 6,127,705 + PS417_28385 0.54 -0.2 6,127,705 + PS417_28385 0.54 -0.7 6,127,705 + PS417_28385 0.54 -0.5 6,127,706 - PS417_28385 0.54 -2.8 6,127,874 + PS417_28385 0.63 +0.4 6,127,874 + PS417_28385 0.63 -0.9 6,127,875 - PS417_28385 0.63 -0.3 6,127,994 - PS417_28385 0.69 +0.1 6,128,386 + +0.8 6,128,386 + -0.6 6,128,387 - -1.1 6,128,602 - +0.8 6,128,641 + -0.7 6,128,642 - +0.7 6,128,642 - +1.9 6,128,658 + -0.1 6,128,659 - -0.4 6,128,659 - +0.4 6,128,773 + -0.5 6,128,774 - -1.4 6,129,029 - PS417_28390 0.43 +0.2 6,129,228 - PS417_28390 0.75 +0.7 6,129,320 - PS417_28390 0.90 +0.5 6,129,320 - PS417_28390 0.90 -0.3
Or see this region's nucleotide sequence