Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02795 and PS417_02800 are separated by 5 nucleotides PS417_02800 and PS417_02805 are separated by 9 nucleotides PS417_02805 and PS417_02810 are separated by 161 nucleotides
PS417_02795: PS417_02795 - acetyltransferase, at 635,389 to 635,922
_02795
PS417_02800: PS417_02800 - acetyltransferase, at 635,928 to 636,440
_02800
PS417_02805: PS417_02805 - urease subunit gamma, at 636,450 to 636,752
_02805
PS417_02810: PS417_02810 - urease accessory protein UreD, at 636,914 to 637,753
_02810
Position (kb) 636
637 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 635.485 kb on - strand, within PS417_02795 at 635.486 kb on + strand, within PS417_02795 at 635.486 kb on + strand, within PS417_02795 at 635.487 kb on - strand, within PS417_02795 at 635.501 kb on + strand, within PS417_02795 at 635.502 kb on - strand, within PS417_02795 at 635.540 kb on + strand, within PS417_02795 at 635.629 kb on + strand, within PS417_02795 at 635.629 kb on + strand, within PS417_02795 at 635.630 kb on - strand, within PS417_02795 at 636.188 kb on - strand, within PS417_02800 at 636.640 kb on + strand, within PS417_02805 at 636.641 kb on - strand, within PS417_02805 at 636.658 kb on + strand, within PS417_02805 at 636.782 kb on + strand at 636.814 kb on + strand at 636.814 kb on + strand at 636.815 kb on - strand at 636.815 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 635,485 - PS417_02795 0.18 -0.8 635,486 + PS417_02795 0.18 +0.7 635,486 + PS417_02795 0.18 +0.2 635,487 - PS417_02795 0.18 -0.1 635,501 + PS417_02795 0.21 -1.2 635,502 - PS417_02795 0.21 +0.5 635,540 + PS417_02795 0.28 +0.2 635,629 + PS417_02795 0.45 +0.4 635,629 + PS417_02795 0.45 -3.3 635,630 - PS417_02795 0.45 +3.3 636,188 - PS417_02800 0.51 -0.4 636,640 + PS417_02805 0.63 -1.4 636,641 - PS417_02805 0.63 -0.8 636,658 + PS417_02805 0.69 -1.0 636,782 + +0.2 636,814 + +0.1 636,814 + +3.4 636,815 - -2.3 636,815 - -2.1
Or see this region's nucleotide sequence