Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28030 and PS417_28035 are separated by 155 nucleotides PS417_28035 and PS417_28040 are separated by 45 nucleotides PS417_28040 and PS417_28050 are separated by 270 nucleotides
PS417_28030: PS417_28030 - hypothetical protein, at 6,049,455 to 6,049,832
_28030
PS417_28035: PS417_28035 - transcriptional regulator PhoB, at 6,049,988 to 6,050,677
_28035
PS417_28040: PS417_28040 - histidine kinase, at 6,050,723 to 6,052,045
_28040
PS417_28050: PS417_28050 - hypothetical protein, at 6,052,316 to 6,053,656
_28050
Position (kb)
6050
6051
6052
6053 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 6049.825 kb on + strand at 6049.825 kb on + strand at 6049.825 kb on + strand at 6049.825 kb on + strand at 6049.825 kb on + strand at 6049.826 kb on - strand at 6049.826 kb on - strand at 6049.826 kb on - strand at 6049.877 kb on - strand at 6049.893 kb on - strand at 6049.933 kb on - strand at 6049.945 kb on + strand at 6049.946 kb on - strand at 6050.069 kb on + strand, within PS417_28035 at 6050.294 kb on + strand, within PS417_28035 at 6050.294 kb on + strand, within PS417_28035 at 6050.295 kb on - strand, within PS417_28035 at 6050.295 kb on - strand, within PS417_28035 at 6050.510 kb on + strand, within PS417_28035 at 6050.555 kb on + strand at 6050.555 kb on + strand, within PS417_28035 at 6050.555 kb on + strand, within PS417_28035 at 6050.556 kb on - strand, within PS417_28035 at 6050.720 kb on + strand at 6050.721 kb on - strand at 6050.807 kb on + strand at 6050.810 kb on + strand at 6050.844 kb on - strand at 6051.377 kb on - strand, within PS417_28040 at 6051.728 kb on + strand, within PS417_28040 at 6051.728 kb on + strand, within PS417_28040 at 6051.729 kb on - strand, within PS417_28040 at 6051.729 kb on - strand, within PS417_28040 at 6051.748 kb on + strand, within PS417_28040 at 6051.979 kb on + strand at 6051.979 kb on + strand at 6051.979 kb on + strand at 6051.979 kb on + strand at 6051.980 kb on - strand at 6051.980 kb on - strand at 6051.985 kb on + strand at 6052.694 kb on + strand, within PS417_28050 at 6052.785 kb on - strand, within PS417_28050 at 6052.896 kb on - strand, within PS417_28050 at 6052.896 kb on - strand, within PS417_28050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 6,049,825 + -0.3 6,049,825 + +1.7 6,049,825 + +3.4 6,049,825 + -0.1 6,049,825 + +1.4 6,049,826 - -1.7 6,049,826 - -1.8 6,049,826 - +0.3 6,049,877 - -2.9 6,049,893 - +0.3 6,049,933 - +1.4 6,049,945 + +2.1 6,049,946 - -0.4 6,050,069 + PS417_28035 0.12 -0.4 6,050,294 + PS417_28035 0.44 +0.1 6,050,294 + PS417_28035 0.44 +1.5 6,050,295 - PS417_28035 0.44 -0.8 6,050,295 - PS417_28035 0.44 +0.9 6,050,510 + PS417_28035 0.76 +0.9 6,050,555 + +2.9 6,050,555 + PS417_28035 0.82 +3.2 6,050,555 + PS417_28035 0.82 +0.2 6,050,556 - PS417_28035 0.82 -3.3 6,050,720 + +0.9 6,050,721 - -2.1 6,050,807 + +1.5 6,050,810 + +0.5 6,050,844 - +1.2 6,051,377 - PS417_28040 0.49 +1.3 6,051,728 + PS417_28040 0.76 -0.3 6,051,728 + PS417_28040 0.76 +0.7 6,051,729 - PS417_28040 0.76 +0.7 6,051,729 - PS417_28040 0.76 -1.7 6,051,748 + PS417_28040 0.77 -1.8 6,051,979 + +0.5 6,051,979 + +1.7 6,051,979 + +1.2 6,051,979 + +1.5 6,051,980 - +1.7 6,051,980 - +0.7 6,051,985 + +0.2 6,052,694 + PS417_28050 0.28 -2.4 6,052,785 - PS417_28050 0.35 -0.6 6,052,896 - PS417_28050 0.43 -3.6 6,052,896 - PS417_28050 0.43 -5.2
Or see this region's nucleotide sequence