Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27285 and PS417_27290 are separated by 19 nucleotides PS417_27290 and PS417_27295 are separated by 138 nucleotides PS417_27295 and PS417_27300 overlap by 4 nucleotides PS417_27300 and PS417_27305 are separated by 265 nucleotides
PS417_27285: PS417_27285 - Xaa-Pro aminopeptidase, at 5,889,603 to 5,890,925
_27285
PS417_27290: PS417_27290 - hypothetical protein, at 5,890,945 to 5,891,502
_27290
PS417_27295: PS417_27295 - hypothetical protein, at 5,891,641 to 5,891,850
_27295
PS417_27300: PS417_27300 - cell division protein ZapA, at 5,891,847 to 5,892,164
_27300
PS417_27305: PS417_27305 - 5-formyltetrahydrofolate cyclo-ligase, at 5,892,430 to 5,893,059
_27305
Position (kb)
5890
5891
5892 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5890.047 kb on + strand, within PS417_27285 at 5890.048 kb on - strand, within PS417_27285 at 5890.050 kb on + strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand at 5890.123 kb on + strand, within PS417_27285 at 5890.401 kb on - strand, within PS417_27285 at 5890.401 kb on - strand, within PS417_27285 at 5890.544 kb on - strand, within PS417_27285 at 5890.544 kb on - strand, within PS417_27285 at 5890.706 kb on - strand, within PS417_27285 at 5890.706 kb on - strand, within PS417_27285 at 5890.900 kb on + strand at 5890.901 kb on - strand at 5890.901 kb on - strand at 5890.901 kb on - strand at 5891.028 kb on - strand, within PS417_27290 at 5891.045 kb on + strand, within PS417_27290 at 5891.046 kb on - strand, within PS417_27290 at 5891.496 kb on + strand at 5891.497 kb on - strand at 5891.533 kb on + strand at 5891.549 kb on - strand at 5891.553 kb on + strand at 5891.554 kb on - strand at 5891.568 kb on + strand at 5891.569 kb on - strand at 5891.577 kb on + strand at 5891.687 kb on + strand, within PS417_27295 at 5891.709 kb on + strand, within PS417_27295 at 5891.729 kb on + strand, within PS417_27295 at 5891.729 kb on + strand, within PS417_27295 at 5891.732 kb on + strand, within PS417_27295 at 5891.732 kb on + strand, within PS417_27295 at 5892.051 kb on - strand, within PS417_27300 at 5892.179 kb on + strand at 5892.187 kb on - strand at 5892.245 kb on + strand at 5892.300 kb on - strand at 5892.345 kb on - strand at 5892.345 kb on - strand at 5892.416 kb on + strand at 5892.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,890,047 + PS417_27285 0.34 -0.4 5,890,048 - PS417_27285 0.34 -1.5 5,890,050 + PS417_27285 0.34 +0.9 5,890,051 - PS417_27285 0.34 -1.6 5,890,051 - PS417_27285 0.34 -0.6 5,890,051 - PS417_27285 0.34 +0.9 5,890,051 - PS417_27285 0.34 -0.2 5,890,051 - PS417_27285 0.34 +0.2 5,890,051 - +0.9 5,890,123 + PS417_27285 0.39 -2.4 5,890,401 - PS417_27285 0.60 -1.2 5,890,401 - PS417_27285 0.60 -0.0 5,890,544 - PS417_27285 0.71 -0.1 5,890,544 - PS417_27285 0.71 +0.0 5,890,706 - PS417_27285 0.83 -1.3 5,890,706 - PS417_27285 0.83 -0.5 5,890,900 + +0.4 5,890,901 - +0.1 5,890,901 - -2.3 5,890,901 - -0.4 5,891,028 - PS417_27290 0.15 -2.7 5,891,045 + PS417_27290 0.18 -3.1 5,891,046 - PS417_27290 0.18 -1.1 5,891,496 + +0.4 5,891,497 - -1.7 5,891,533 + -2.2 5,891,549 - -1.1 5,891,553 + -0.7 5,891,554 - -2.5 5,891,568 + +0.8 5,891,569 - -0.7 5,891,577 + -0.5 5,891,687 + PS417_27295 0.22 -0.5 5,891,709 + PS417_27295 0.32 -3.9 5,891,729 + PS417_27295 0.42 +0.5 5,891,729 + PS417_27295 0.42 +1.0 5,891,732 + PS417_27295 0.43 +0.8 5,891,732 + PS417_27295 0.43 +1.1 5,892,051 - PS417_27300 0.64 -3.3 5,892,179 + -0.8 5,892,187 - -3.0 5,892,245 + -2.1 5,892,300 - +1.0 5,892,345 - -1.1 5,892,345 - +0.2 5,892,416 + -1.3 5,892,417 + +0.1
Or see this region's nucleotide sequence