Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27105 and PS417_27110 overlap by 4 nucleotides PS417_27110 and PS417_27115 overlap by 4 nucleotides PS417_27115 and PS417_27120 are separated by 66 nucleotides
PS417_27105: PS417_27105 - spermidine/putrescine ABC transporter permease, at 5,851,023 to 5,851,907
_27105
PS417_27110: PS417_27110 - spermidine/putrescine ABC transporter permease, at 5,851,904 to 5,852,824
_27110
PS417_27115: PS417_27115 - putrescine ABC transporter, ATPase component <small>(from data)</small>, at 5,852,821 to 5,853,963
_27115
PS417_27120: PS417_27120 - ABC transporter substrate-binding protein, at 5,854,030 to 5,855,124
_27120
Position (kb) 5852
5853
5854 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5851.825 kb on + strand at 5851.853 kb on - strand at 5851.853 kb on - strand at 5851.856 kb on + strand at 5851.857 kb on - strand at 5851.907 kb on + strand at 5851.908 kb on - strand at 5852.280 kb on + strand, within PS417_27110 at 5852.281 kb on - strand, within PS417_27110 at 5852.281 kb on - strand, within PS417_27110 at 5852.281 kb on - strand, within PS417_27110 at 5852.295 kb on + strand, within PS417_27110 at 5852.296 kb on - strand, within PS417_27110 at 5852.296 kb on - strand, within PS417_27110 at 5852.297 kb on + strand, within PS417_27110 at 5852.403 kb on + strand, within PS417_27110 at 5852.601 kb on + strand, within PS417_27110 at 5852.833 kb on + strand at 5852.864 kb on + strand at 5852.864 kb on + strand at 5852.865 kb on - strand at 5852.865 kb on - strand at 5852.865 kb on - strand at 5852.865 kb on - strand at 5852.955 kb on - strand, within PS417_27115 at 5852.955 kb on - strand, within PS417_27115 at 5852.955 kb on - strand, within PS417_27115 at 5852.955 kb on - strand, within PS417_27115 at 5852.978 kb on + strand, within PS417_27115 at 5853.116 kb on + strand, within PS417_27115 at 5853.117 kb on - strand, within PS417_27115 at 5853.117 kb on - strand, within PS417_27115 at 5853.117 kb on - strand, within PS417_27115 at 5853.117 kb on - strand, within PS417_27115 at 5853.242 kb on + strand, within PS417_27115 at 5853.639 kb on + strand, within PS417_27115 at 5853.639 kb on + strand, within PS417_27115 at 5853.639 kb on + strand, within PS417_27115 at 5853.639 kb on + strand, within PS417_27115 at 5853.726 kb on - strand, within PS417_27115 at 5853.939 kb on + strand at 5853.941 kb on + strand at 5853.966 kb on - strand at 5854.031 kb on + strand at 5854.032 kb on - strand at 5854.032 kb on - strand at 5854.032 kb on - strand at 5854.211 kb on + strand, within PS417_27120 at 5854.212 kb on - strand, within PS417_27120 at 5854.250 kb on + strand, within PS417_27120 at 5854.250 kb on + strand, within PS417_27120 at 5854.347 kb on - strand, within PS417_27120 at 5854.457 kb on + strand, within PS417_27120 at 5854.457 kb on + strand, within PS417_27120 at 5854.475 kb on + strand, within PS417_27120 at 5854.476 kb on - strand, within PS417_27120 at 5854.476 kb on - strand, within PS417_27120 at 5854.539 kb on + strand, within PS417_27120 at 5854.540 kb on - strand, within PS417_27120 at 5854.562 kb on + strand, within PS417_27120 at 5854.562 kb on + strand, within PS417_27120 at 5854.563 kb on - strand, within PS417_27120 at 5854.563 kb on - strand, within PS417_27120 at 5854.739 kb on + strand, within PS417_27120 at 5854.752 kb on - strand, within PS417_27120 at 5854.961 kb on + strand, within PS417_27120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,851,825 + -1.8 5,851,853 - -1.3 5,851,853 - +1.9 5,851,856 + +1.2 5,851,857 - +1.7 5,851,907 + -0.1 5,851,908 - -1.1 5,852,280 + PS417_27110 0.41 -0.3 5,852,281 - PS417_27110 0.41 -2.1 5,852,281 - PS417_27110 0.41 -2.2 5,852,281 - PS417_27110 0.41 -1.4 5,852,295 + PS417_27110 0.42 -1.0 5,852,296 - PS417_27110 0.43 -4.1 5,852,296 - PS417_27110 0.43 -2.8 5,852,297 + PS417_27110 0.43 -2.1 5,852,403 + PS417_27110 0.54 -1.1 5,852,601 + PS417_27110 0.76 +1.0 5,852,833 + -1.3 5,852,864 + -0.6 5,852,864 + -1.2 5,852,865 - +1.2 5,852,865 - -0.2 5,852,865 - -1.5 5,852,865 - -1.0 5,852,955 - PS417_27115 0.12 -0.5 5,852,955 - PS417_27115 0.12 -2.5 5,852,955 - PS417_27115 0.12 -1.3 5,852,955 - PS417_27115 0.12 -2.7 5,852,978 + PS417_27115 0.14 -1.3 5,853,116 + PS417_27115 0.26 -1.7 5,853,117 - PS417_27115 0.26 -3.4 5,853,117 - PS417_27115 0.26 -0.8 5,853,117 - PS417_27115 0.26 -0.5 5,853,117 - PS417_27115 0.26 -0.1 5,853,242 + PS417_27115 0.37 -1.5 5,853,639 + PS417_27115 0.72 -0.5 5,853,639 + PS417_27115 0.72 -0.7 5,853,639 + PS417_27115 0.72 -1.5 5,853,639 + PS417_27115 0.72 +0.4 5,853,726 - PS417_27115 0.79 +0.1 5,853,939 + -2.2 5,853,941 + -2.7 5,853,966 - -1.6 5,854,031 + -0.6 5,854,032 - -0.1 5,854,032 - +0.6 5,854,032 - +0.3 5,854,211 + PS417_27120 0.17 -2.0 5,854,212 - PS417_27120 0.17 -3.2 5,854,250 + PS417_27120 0.20 +0.6 5,854,250 + PS417_27120 0.20 -2.5 5,854,347 - PS417_27120 0.29 +0.3 5,854,457 + PS417_27120 0.39 -0.2 5,854,457 + PS417_27120 0.39 -0.9 5,854,475 + PS417_27120 0.41 -1.3 5,854,476 - PS417_27120 0.41 +0.6 5,854,476 - PS417_27120 0.41 -2.0 5,854,539 + PS417_27120 0.46 -0.3 5,854,540 - PS417_27120 0.47 -0.0 5,854,562 + PS417_27120 0.49 -1.1 5,854,562 + PS417_27120 0.49 +3.2 5,854,563 - PS417_27120 0.49 +0.5 5,854,563 - PS417_27120 0.49 +1.0 5,854,739 + PS417_27120 0.65 -2.3 5,854,752 - PS417_27120 0.66 -0.7 5,854,961 + PS417_27120 0.85 -2.3
Or see this region's nucleotide sequence