Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26845 and PS417_26850 are separated by 28 nucleotides PS417_26850 and PS417_26855 are separated by 85 nucleotides PS417_26855 and PS417_26860 are separated by 114 nucleotides PS417_26860 and PS417_26865 are separated by 103 nucleotides
PS417_26845: PS417_26845 - coniferyl aldehyde dehydrogenase, at 5,796,347 to 5,797,774
_26845
PS417_26850: PS417_26850 - twin-arginine translocation pathway signal protein, at 5,797,803 to 5,798,348
_26850
PS417_26855: PS417_26855 - GMC family oxidoreductase, at 5,798,434 to 5,800,029
_26855
PS417_26860: PS417_26860 - phosphopantetheine adenylyltransferase, at 5,800,144 to 5,800,623
_26860
PS417_26865: PS417_26865 - ferredoxin, at 5,800,727 to 5,800,978
_26865
Position (kb) 5798
5799
5800
5801 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5797.457 kb on + strand, within PS417_26845 at 5797.458 kb on - strand, within PS417_26845 at 5797.458 kb on - strand, within PS417_26845 at 5797.458 kb on - strand, within PS417_26845 at 5797.490 kb on + strand, within PS417_26845 at 5797.491 kb on - strand, within PS417_26845 at 5797.493 kb on + strand, within PS417_26845 at 5797.494 kb on - strand, within PS417_26845 at 5797.494 kb on - strand, within PS417_26845 at 5797.494 kb on - strand, within PS417_26845 at 5797.494 kb on - strand, within PS417_26845 at 5797.959 kb on - strand, within PS417_26850 at 5798.346 kb on + strand at 5798.412 kb on - strand at 5798.665 kb on + strand, within PS417_26855 at 5798.665 kb on + strand, within PS417_26855 at 5798.666 kb on - strand, within PS417_26855 at 5798.666 kb on - strand, within PS417_26855 at 5798.666 kb on - strand, within PS417_26855 at 5798.782 kb on - strand, within PS417_26855 at 5798.835 kb on + strand, within PS417_26855 at 5798.927 kb on + strand, within PS417_26855 at 5798.928 kb on - strand, within PS417_26855 at 5798.980 kb on - strand, within PS417_26855 at 5799.007 kb on + strand, within PS417_26855 at 5799.097 kb on + strand, within PS417_26855 at 5799.220 kb on + strand, within PS417_26855 at 5799.220 kb on + strand, within PS417_26855 at 5799.220 kb on + strand, within PS417_26855 at 5799.220 kb on + strand, within PS417_26855 at 5799.221 kb on - strand, within PS417_26855 at 5799.368 kb on - strand, within PS417_26855 at 5799.389 kb on - strand, within PS417_26855 at 5799.389 kb on - strand, within PS417_26855 at 5799.437 kb on - strand, within PS417_26855 at 5799.437 kb on - strand, within PS417_26855 at 5799.437 kb on - strand, within PS417_26855 at 5799.631 kb on + strand, within PS417_26855 at 5799.632 kb on - strand, within PS417_26855 at 5799.646 kb on + strand, within PS417_26855 at 5799.745 kb on + strand, within PS417_26855 at 5799.746 kb on - strand, within PS417_26855 at 5799.746 kb on - strand, within PS417_26855 at 5799.973 kb on + strand at 5799.973 kb on + strand at 5800.041 kb on - strand at 5800.053 kb on + strand at 5800.053 kb on + strand at 5800.053 kb on + strand at 5800.054 kb on - strand at 5800.054 kb on - strand at 5800.054 kb on - strand at 5800.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,797,457 + PS417_26845 0.78 +0.8 5,797,458 - PS417_26845 0.78 -1.8 5,797,458 - PS417_26845 0.78 -0.3 5,797,458 - PS417_26845 0.78 -2.7 5,797,490 + PS417_26845 0.80 -0.3 5,797,491 - PS417_26845 0.80 +1.3 5,797,493 + PS417_26845 0.80 -0.8 5,797,494 - PS417_26845 0.80 +1.8 5,797,494 - PS417_26845 0.80 +0.4 5,797,494 - PS417_26845 0.80 -0.9 5,797,494 - PS417_26845 0.80 +0.3 5,797,959 - PS417_26850 0.29 +0.7 5,798,346 + -0.5 5,798,412 - +2.0 5,798,665 + PS417_26855 0.14 +0.9 5,798,665 + PS417_26855 0.14 +0.8 5,798,666 - PS417_26855 0.15 -0.7 5,798,666 - PS417_26855 0.15 -0.2 5,798,666 - PS417_26855 0.15 -2.2 5,798,782 - PS417_26855 0.22 +0.6 5,798,835 + PS417_26855 0.25 +2.0 5,798,927 + PS417_26855 0.31 +2.6 5,798,928 - PS417_26855 0.31 +0.2 5,798,980 - PS417_26855 0.34 -0.7 5,799,007 + PS417_26855 0.36 +1.4 5,799,097 + PS417_26855 0.42 +0.8 5,799,220 + PS417_26855 0.49 +0.7 5,799,220 + PS417_26855 0.49 +0.3 5,799,220 + PS417_26855 0.49 +0.3 5,799,220 + PS417_26855 0.49 +0.1 5,799,221 - PS417_26855 0.49 -2.2 5,799,368 - PS417_26855 0.59 +0.8 5,799,389 - PS417_26855 0.60 -1.2 5,799,389 - PS417_26855 0.60 -0.2 5,799,437 - PS417_26855 0.63 -0.3 5,799,437 - PS417_26855 0.63 +2.9 5,799,437 - PS417_26855 0.63 +1.3 5,799,631 + PS417_26855 0.75 +1.0 5,799,632 - PS417_26855 0.75 -0.2 5,799,646 + PS417_26855 0.76 +0.1 5,799,745 + PS417_26855 0.82 -0.1 5,799,746 - PS417_26855 0.82 -1.6 5,799,746 - PS417_26855 0.82 -0.2 5,799,973 + +1.2 5,799,973 + -0.3 5,800,041 - +0.3 5,800,053 + +1.3 5,800,053 + +0.8 5,800,053 + +0.3 5,800,054 - -0.8 5,800,054 - -0.0 5,800,054 - -0.7 5,800,621 + +0.5
Or see this region's nucleotide sequence