Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26745 and PS417_26750 are separated by 21 nucleotides PS417_26750 and PS417_26755 overlap by 4 nucleotides PS417_26755 and PS417_26760 overlap by 4 nucleotides
PS417_26745: PS417_26745 - methionine biosynthesis protein MetW, at 5,779,304 to 5,779,924
_26745
PS417_26750: PS417_26750 - homoserine acetyltransferase, at 5,779,946 to 5,780,380
_26750
PS417_26755: PS417_26755 - nucleoside-triphosphate diphosphatase, at 5,780,377 to 5,780,973
_26755
PS417_26760: PS417_26760 - oxidase, at 5,780,970 to 5,782,172
_26760
Position (kb)
5780
5781 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5779.709 kb on + strand, within PS417_26745 at 5779.719 kb on - strand, within PS417_26745 at 5779.719 kb on - strand, within PS417_26745 at 5779.719 kb on - strand, within PS417_26745 at 5779.719 kb on - strand, within PS417_26745 at 5779.841 kb on - strand, within PS417_26745 at 5779.845 kb on + strand, within PS417_26745 at 5779.845 kb on + strand, within PS417_26745 at 5779.845 kb on + strand, within PS417_26745 at 5779.845 kb on + strand, within PS417_26745 at 5779.922 kb on + strand at 5779.923 kb on - strand at 5780.012 kb on - strand, within PS417_26750 at 5780.013 kb on + strand, within PS417_26750 at 5780.013 kb on + strand, within PS417_26750 at 5780.014 kb on - strand, within PS417_26750 at 5780.057 kb on + strand, within PS417_26750 at 5780.058 kb on - strand, within PS417_26750 at 5780.115 kb on + strand, within PS417_26750 at 5780.308 kb on + strand, within PS417_26750 at 5780.388 kb on + strand at 5780.400 kb on - strand at 5780.632 kb on + strand, within PS417_26755 at 5780.845 kb on + strand, within PS417_26755 at 5781.061 kb on - strand at 5781.147 kb on + strand, within PS417_26760 at 5781.147 kb on + strand, within PS417_26760 at 5781.148 kb on - strand, within PS417_26760 at 5781.148 kb on - strand, within PS417_26760 at 5781.738 kb on + strand, within PS417_26760 at 5781.845 kb on + strand, within PS417_26760 at 5781.900 kb on + strand, within PS417_26760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,779,709 + PS417_26745 0.65 +1.3 5,779,719 - PS417_26745 0.67 +1.2 5,779,719 - PS417_26745 0.67 +0.0 5,779,719 - PS417_26745 0.67 +0.5 5,779,719 - PS417_26745 0.67 +0.3 5,779,841 - PS417_26745 0.86 +0.6 5,779,845 + PS417_26745 0.87 +1.7 5,779,845 + PS417_26745 0.87 -1.0 5,779,845 + PS417_26745 0.87 -1.2 5,779,845 + PS417_26745 0.87 -1.2 5,779,922 + -0.2 5,779,923 - +0.8 5,780,012 - PS417_26750 0.15 +0.5 5,780,013 + PS417_26750 0.15 -2.6 5,780,013 + PS417_26750 0.15 +0.2 5,780,014 - PS417_26750 0.16 +1.4 5,780,057 + PS417_26750 0.26 +0.2 5,780,058 - PS417_26750 0.26 +0.1 5,780,115 + PS417_26750 0.39 +0.2 5,780,308 + PS417_26750 0.83 -1.5 5,780,388 + -2.0 5,780,400 - +0.5 5,780,632 + PS417_26755 0.43 -1.5 5,780,845 + PS417_26755 0.78 -2.7 5,781,061 - -0.8 5,781,147 + PS417_26760 0.15 -0.7 5,781,147 + PS417_26760 0.15 -2.5 5,781,148 - PS417_26760 0.15 -2.2 5,781,148 - PS417_26760 0.15 +0.1 5,781,738 + PS417_26760 0.64 +0.8 5,781,845 + PS417_26760 0.73 -2.5 5,781,900 + PS417_26760 0.77 -3.0
Or see this region's nucleotide sequence