Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26450 and PS417_26455 are separated by 210 nucleotides PS417_26455 and PS417_26460 are separated by 95 nucleotides PS417_26460 and PS417_26465 are separated by 39 nucleotides PS417_26465 and PS417_26470 are separated by 6 nucleotides
PS417_26450: PS417_26450 - S-adenosylmethionine synthetase, at 5,720,606 to 5,721,796
_26450
PS417_26455: PS417_26455 - hypothetical protein, at 5,722,007 to 5,722,357
_26455
PS417_26460: PS417_26460 - NAD-dependent DNA ligase LigB, at 5,722,453 to 5,724,117
_26460
PS417_26465: PS417_26465 - hypothetical protein, at 5,724,157 to 5,724,552
_26465
PS417_26470: PS417_26470 - cytochrome C, at 5,724,559 to 5,725,008
_26470
Position (kb)
5722
5723
5724
5725 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5721.795 kb on - strand at 5721.846 kb on + strand at 5721.846 kb on + strand at 5721.894 kb on - strand at 5721.934 kb on - strand at 5721.934 kb on - strand at 5721.934 kb on - strand at 5721.938 kb on + strand at 5722.315 kb on + strand, within PS417_26455 at 5722.355 kb on + strand at 5722.356 kb on - strand at 5722.359 kb on + strand at 5722.360 kb on - strand at 5722.365 kb on + strand at 5722.365 kb on + strand at 5722.372 kb on + strand at 5722.421 kb on - strand at 5722.478 kb on + strand at 5722.479 kb on - strand at 5722.562 kb on + strand at 5722.562 kb on + strand at 5722.562 kb on + strand at 5722.562 kb on + strand at 5722.582 kb on + strand at 5722.582 kb on + strand at 5722.582 kb on + strand at 5722.583 kb on - strand at 5722.621 kb on + strand, within PS417_26460 at 5722.622 kb on - strand, within PS417_26460 at 5722.622 kb on - strand, within PS417_26460 at 5722.738 kb on - strand, within PS417_26460 at 5723.249 kb on - strand, within PS417_26460 at 5723.396 kb on + strand, within PS417_26460 at 5723.397 kb on - strand, within PS417_26460 at 5723.741 kb on + strand, within PS417_26460 at 5723.742 kb on - strand, within PS417_26460 at 5723.762 kb on + strand, within PS417_26460 at 5723.946 kb on + strand, within PS417_26460 at 5723.947 kb on - strand, within PS417_26460 at 5724.068 kb on + strand at 5724.068 kb on + strand at 5724.069 kb on - strand at 5724.069 kb on - strand at 5724.090 kb on - strand at 5724.490 kb on - strand, within PS417_26465 at 5724.641 kb on + strand, within PS417_26470 at 5724.642 kb on - strand, within PS417_26470 at 5724.983 kb on - strand at 5724.983 kb on - strand at 5725.075 kb on + strand at 5725.075 kb on + strand at 5725.078 kb on + strand at 5725.079 kb on - strand at 5725.081 kb on + strand at 5725.081 kb on + strand at 5725.082 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,721,795 - -0.5 5,721,846 + +0.1 5,721,846 + +0.2 5,721,894 - -3.3 5,721,934 - -1.6 5,721,934 - +0.3 5,721,934 - -0.5 5,721,938 + -0.5 5,722,315 + PS417_26455 0.88 -0.1 5,722,355 + -3.6 5,722,356 - +0.2 5,722,359 + +1.2 5,722,360 - -1.2 5,722,365 + -0.9 5,722,365 + -0.2 5,722,372 + +0.6 5,722,421 - -3.1 5,722,478 + +1.2 5,722,479 - +0.8 5,722,562 + -0.2 5,722,562 + -0.5 5,722,562 + -0.3 5,722,562 + +2.1 5,722,582 + -2.3 5,722,582 + -3.4 5,722,582 + -1.1 5,722,583 - -0.7 5,722,621 + PS417_26460 0.10 -1.6 5,722,622 - PS417_26460 0.10 +0.1 5,722,622 - PS417_26460 0.10 -2.9 5,722,738 - PS417_26460 0.17 +0.1 5,723,249 - PS417_26460 0.48 -2.1 5,723,396 + PS417_26460 0.57 -1.8 5,723,397 - PS417_26460 0.57 +1.5 5,723,741 + PS417_26460 0.77 +0.1 5,723,742 - PS417_26460 0.77 -0.1 5,723,762 + PS417_26460 0.79 -1.4 5,723,946 + PS417_26460 0.90 -0.5 5,723,947 - PS417_26460 0.90 -0.5 5,724,068 + -0.2 5,724,068 + +1.0 5,724,069 - -1.6 5,724,069 - -1.4 5,724,090 - +0.7 5,724,490 - PS417_26465 0.84 +0.8 5,724,641 + PS417_26470 0.18 +1.0 5,724,642 - PS417_26470 0.18 +0.8 5,724,983 - -0.1 5,724,983 - -0.4 5,725,075 + +0.7 5,725,075 + +2.4 5,725,078 + +0.7 5,725,079 - +4.2 5,725,081 + -2.7 5,725,081 + -1.3 5,725,082 - +0.5
Or see this region's nucleotide sequence