Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26280 and PS417_26285 are separated by 142 nucleotides PS417_26285 and PS417_26290 are separated by 14 nucleotides PS417_26290 and PS417_26295 are separated by 27 nucleotides
PS417_26280: PS417_26280 - NADPH:quinone reductase, at 5,679,418 to 5,680,305
_26280
PS417_26285: PS417_26285 - 3-hydroxybutyryl-CoA dehydrogenase, at 5,680,448 to 5,681,413
_26285
PS417_26290: PS417_26290 - 4-hydroxybenzoyl-CoA thioesterase, at 5,681,428 to 5,681,904
_26290
PS417_26295: PS417_26295 - gamma-butyrobetaine dioxygenase, at 5,681,932 to 5,683,092
_26295
Position (kb) 5680
5681
5682 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5679.841 kb on + strand, within PS417_26280 at 5679.841 kb on + strand, within PS417_26280 at 5679.842 kb on - strand, within PS417_26280 at 5679.842 kb on - strand, within PS417_26280 at 5679.850 kb on - strand, within PS417_26280 at 5680.358 kb on - strand at 5680.962 kb on - strand, within PS417_26285 at 5681.156 kb on + strand, within PS417_26285 at 5681.156 kb on + strand, within PS417_26285 at 5681.157 kb on - strand, within PS417_26285 at 5681.157 kb on - strand, within PS417_26285 at 5681.234 kb on + strand, within PS417_26285 at 5681.235 kb on - strand, within PS417_26285 at 5681.235 kb on - strand, within PS417_26285 at 5681.446 kb on + strand at 5681.446 kb on + strand at 5681.446 kb on + strand at 5681.446 kb on + strand at 5681.480 kb on - strand, within PS417_26290 at 5681.621 kb on - strand, within PS417_26290 at 5681.621 kb on - strand, within PS417_26290 at 5681.621 kb on - strand, within PS417_26290 at 5681.662 kb on - strand, within PS417_26290 at 5681.915 kb on + strand at 5681.922 kb on - strand at 5681.965 kb on - strand at 5681.965 kb on - strand at 5681.996 kb on + strand at 5682.098 kb on + strand at 5682.098 kb on + strand, within PS417_26295 at 5682.098 kb on + strand, within PS417_26295 at 5682.100 kb on + strand, within PS417_26295 at 5682.101 kb on - strand, within PS417_26295 at 5682.101 kb on - strand, within PS417_26295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,679,841 + PS417_26280 0.48 -0.2 5,679,841 + PS417_26280 0.48 +2.8 5,679,842 - PS417_26280 0.48 -1.0 5,679,842 - PS417_26280 0.48 +0.7 5,679,850 - PS417_26280 0.49 +0.6 5,680,358 - -1.5 5,680,962 - PS417_26285 0.53 +0.1 5,681,156 + PS417_26285 0.73 +2.0 5,681,156 + PS417_26285 0.73 +1.1 5,681,157 - PS417_26285 0.73 +0.4 5,681,157 - PS417_26285 0.73 +0.4 5,681,234 + PS417_26285 0.81 +0.6 5,681,235 - PS417_26285 0.81 -2.1 5,681,235 - PS417_26285 0.81 +0.7 5,681,446 + -0.4 5,681,446 + +1.1 5,681,446 + -2.1 5,681,446 + -3.9 5,681,480 - PS417_26290 0.11 -2.6 5,681,621 - PS417_26290 0.40 -0.5 5,681,621 - PS417_26290 0.40 +0.1 5,681,621 - PS417_26290 0.40 +1.0 5,681,662 - PS417_26290 0.49 -1.3 5,681,915 + -1.4 5,681,922 - +1.8 5,681,965 - +1.6 5,681,965 - -0.4 5,681,996 + -0.2 5,682,098 + +0.8 5,682,098 + PS417_26295 0.14 +0.2 5,682,098 + PS417_26295 0.14 +1.1 5,682,100 + PS417_26295 0.14 +0.2 5,682,101 - PS417_26295 0.15 +0.1 5,682,101 - PS417_26295 0.15 -1.0
Or see this region's nucleotide sequence