Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26170 and PS417_26175 are separated by 10 nucleotides PS417_26175 and PS417_26180 are separated by 123 nucleotides
PS417_26170: PS417_26170 - formyltetrahydrofolate deformylase, at 5,654,431 to 5,655,288
_26170
PS417_26175: PS417_26175 - sarcosine oxidase subunit gamma, at 5,655,299 to 5,655,931
_26175
PS417_26180: PS417_26180 - sarcosine oxidase subunit alpha, at 5,656,055 to 5,659,072
_26180
Position (kb)
5655
5656 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5654.330 kb on + strand at 5654.331 kb on - strand at 5654.331 kb on - strand at 5654.333 kb on + strand at 5654.334 kb on - strand at 5654.334 kb on - strand at 5654.433 kb on - strand at 5654.618 kb on + strand, within PS417_26170 at 5654.618 kb on + strand, within PS417_26170 at 5654.619 kb on - strand, within PS417_26170 at 5654.619 kb on - strand, within PS417_26170 at 5654.658 kb on - strand, within PS417_26170 at 5654.658 kb on - strand, within PS417_26170 at 5654.658 kb on - strand, within PS417_26170 at 5654.769 kb on - strand, within PS417_26170 at 5654.868 kb on - strand, within PS417_26170 at 5654.868 kb on - strand, within PS417_26170 at 5654.967 kb on - strand, within PS417_26170 at 5655.027 kb on - strand, within PS417_26170 at 5655.027 kb on - strand, within PS417_26170 at 5655.184 kb on - strand, within PS417_26170 at 5655.188 kb on + strand, within PS417_26170 at 5655.188 kb on + strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.293 kb on + strand at 5655.486 kb on + strand, within PS417_26175 at 5655.486 kb on + strand, within PS417_26175 at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.915 kb on - strand at 5655.915 kb on - strand at 5655.915 kb on - strand at 5655.932 kb on - strand at 5656.342 kb on + strand at 5656.409 kb on - strand, within PS417_26180 at 5656.502 kb on + strand, within PS417_26180 at 5656.503 kb on - strand, within PS417_26180 at 5656.571 kb on + strand, within PS417_26180 at 5656.585 kb on - strand, within PS417_26180 at 5656.640 kb on + strand, within PS417_26180 at 5656.671 kb on - strand, within PS417_26180 at 5656.797 kb on + strand, within PS417_26180 at 5656.798 kb on - strand, within PS417_26180 at 5656.830 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,654,330 + -1.8 5,654,331 - +0.2 5,654,331 - -0.7 5,654,333 + -2.6 5,654,334 - +0.8 5,654,334 - +2.5 5,654,433 - -0.2 5,654,618 + PS417_26170 0.22 -0.5 5,654,618 + PS417_26170 0.22 +1.8 5,654,619 - PS417_26170 0.22 +1.0 5,654,619 - PS417_26170 0.22 +0.9 5,654,658 - PS417_26170 0.26 -0.1 5,654,658 - PS417_26170 0.26 -1.5 5,654,658 - PS417_26170 0.26 -0.2 5,654,769 - PS417_26170 0.39 -1.2 5,654,868 - PS417_26170 0.51 +0.0 5,654,868 - PS417_26170 0.51 +0.1 5,654,967 - PS417_26170 0.62 +0.3 5,655,027 - PS417_26170 0.69 +1.2 5,655,027 - PS417_26170 0.69 -2.5 5,655,184 - PS417_26170 0.88 -3.4 5,655,188 + PS417_26170 0.88 -0.2 5,655,188 + PS417_26170 0.88 +1.0 5,655,189 - PS417_26170 0.88 -0.2 5,655,189 - PS417_26170 0.88 -0.6 5,655,189 - PS417_26170 0.88 +0.5 5,655,293 + +1.3 5,655,486 + PS417_26175 0.30 -1.1 5,655,486 + PS417_26175 0.30 -2.4 5,655,914 + -4.3 5,655,914 + +2.2 5,655,914 + +1.1 5,655,914 + +1.2 5,655,914 + -0.4 5,655,915 - -0.8 5,655,915 - -0.5 5,655,915 - -0.2 5,655,932 - +0.7 5,656,342 + -1.1 5,656,409 - PS417_26180 0.12 +2.5 5,656,502 + PS417_26180 0.15 -1.0 5,656,503 - PS417_26180 0.15 -0.6 5,656,571 + PS417_26180 0.17 +0.2 5,656,585 - PS417_26180 0.18 +0.6 5,656,640 + PS417_26180 0.19 -2.9 5,656,671 - PS417_26180 0.20 +1.7 5,656,797 + PS417_26180 0.25 +1.4 5,656,798 - PS417_26180 0.25 +2.0 5,656,830 + -2.0
Or see this region's nucleotide sequence