Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02595 and PS417_02600 overlap by 4 nucleotides PS417_02600 and PS417_02605 are separated by 187 nucleotides
PS417_02595: PS417_02595 - NAD synthetase, at 591,743 to 592,675
_02595
PS417_02600: PS417_02600 - hypothetical protein, at 592,672 to 593,121
_02600
PS417_02605: PS417_02605 - IMP dehydrogenase, at 593,309 to 596,599
_02605
Position (kb) 592
593
594 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.887 kb on + strand, within PS417_02595 at 591.887 kb on + strand, within PS417_02595 at 591.888 kb on - strand, within PS417_02595 at 591.888 kb on - strand, within PS417_02595 at 591.984 kb on - strand, within PS417_02595 at 592.169 kb on - strand, within PS417_02595 at 592.219 kb on + strand, within PS417_02595 at 592.219 kb on + strand, within PS417_02595 at 592.219 kb on + strand, within PS417_02595 at 592.220 kb on - strand, within PS417_02595 at 592.220 kb on - strand, within PS417_02595 at 592.223 kb on + strand, within PS417_02595 at 592.223 kb on + strand, within PS417_02595 at 592.224 kb on - strand, within PS417_02595 at 592.224 kb on - strand, within PS417_02595 at 592.224 kb on - strand, within PS417_02595 at 592.277 kb on + strand, within PS417_02595 at 592.295 kb on - strand, within PS417_02595 at 592.454 kb on - strand, within PS417_02595 at 592.486 kb on - strand, within PS417_02595 at 592.609 kb on + strand at 592.609 kb on + strand at 592.610 kb on - strand at 592.610 kb on - strand at 592.610 kb on - strand at 592.623 kb on + strand at 592.624 kb on - strand at 592.624 kb on - strand at 592.655 kb on + strand at 592.789 kb on + strand, within PS417_02600 at 592.789 kb on + strand, within PS417_02600 at 592.789 kb on + strand at 592.825 kb on + strand, within PS417_02600 at 592.826 kb on - strand, within PS417_02600 at 592.842 kb on - strand, within PS417_02600 at 592.847 kb on + strand, within PS417_02600 at 592.925 kb on - strand, within PS417_02600 at 593.075 kb on + strand, within PS417_02600 at 593.127 kb on + strand at 593.190 kb on + strand at 593.214 kb on + strand at 593.215 kb on - strand at 593.229 kb on + strand at 593.261 kb on + strand at 593.262 kb on - strand at 593.345 kb on + strand at 593.352 kb on - strand at 593.492 kb on + strand at 593.492 kb on + strand at 593.532 kb on + strand at 593.532 kb on + strand at 593.533 kb on - strand at 593.533 kb on - strand at 593.625 kb on - strand at 593.696 kb on + strand, within PS417_02605 at 593.710 kb on - strand, within PS417_02605 at 594.011 kb on + strand, within PS417_02605 at 594.011 kb on + strand, within PS417_02605 at 594.011 kb on + strand, within PS417_02605 at 594.078 kb on + strand, within PS417_02605 at 594.078 kb on + strand, within PS417_02605 at 594.078 kb on + strand, within PS417_02605 at 594.078 kb on + strand, within PS417_02605
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 591,742 + +0.0 591,742 + +1.1 591,742 + +0.9 591,742 + -0.0 591,743 - -1.8 591,743 - -2.9 591,887 + PS417_02595 0.15 -0.1 591,887 + PS417_02595 0.15 +1.9 591,888 - PS417_02595 0.16 +0.3 591,888 - PS417_02595 0.16 +0.2 591,984 - PS417_02595 0.26 -0.1 592,169 - PS417_02595 0.46 -3.5 592,219 + PS417_02595 0.51 -0.1 592,219 + PS417_02595 0.51 -3.3 592,219 + PS417_02595 0.51 -1.0 592,220 - PS417_02595 0.51 +0.5 592,220 - PS417_02595 0.51 +0.6 592,223 + PS417_02595 0.51 +0.2 592,223 + PS417_02595 0.51 +1.8 592,224 - PS417_02595 0.52 +0.6 592,224 - PS417_02595 0.52 +0.7 592,224 - PS417_02595 0.52 +3.1 592,277 + PS417_02595 0.57 -0.5 592,295 - PS417_02595 0.59 +0.6 592,454 - PS417_02595 0.76 +0.6 592,486 - PS417_02595 0.80 -2.4 592,609 + +0.3 592,609 + -0.6 592,610 - +0.2 592,610 - +0.9 592,610 - +1.0 592,623 + -0.5 592,624 - -1.0 592,624 - +1.9 592,655 + -0.7 592,789 + PS417_02600 0.26 -1.4 592,789 + PS417_02600 0.26 -0.6 592,789 + -0.5 592,825 + PS417_02600 0.34 -1.3 592,826 - PS417_02600 0.34 -0.3 592,842 - PS417_02600 0.38 -1.6 592,847 + PS417_02600 0.39 -2.0 592,925 - PS417_02600 0.56 +0.3 593,075 + PS417_02600 0.90 +0.7 593,127 + -0.2 593,190 + +0.1 593,214 + -1.4 593,215 - -1.1 593,229 + -0.6 593,261 + -0.7 593,262 - +1.1 593,345 + +0.7 593,352 - +0.4 593,492 + +0.5 593,492 + +0.4 593,532 + +0.5 593,532 + +1.4 593,533 - +1.2 593,533 - +0.3 593,625 - -0.9 593,696 + PS417_02605 0.12 +1.0 593,710 - PS417_02605 0.12 +0.6 594,011 + PS417_02605 0.21 +0.6 594,011 + PS417_02605 0.21 +2.3 594,011 + PS417_02605 0.21 +1.1 594,078 + PS417_02605 0.23 -2.9 594,078 + PS417_02605 0.23 +0.5 594,078 + PS417_02605 0.23 +0.0 594,078 + PS417_02605 0.23 -0.8
Or see this region's nucleotide sequence