Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02585 and PS417_02590 are separated by 96 nucleotides PS417_02590 and PS417_02595 are separated by 118 nucleotides
PS417_02585: PS417_02585 - DNA helicase, at 587,827 to 589,224
_02585
PS417_02590: PS417_02590 - hypothetical protein, at 589,321 to 591,624
_02590
PS417_02595: PS417_02595 - NAD synthetase, at 591,743 to 592,675
_02595
Position (kb) 589
590
591
592 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 589.201 kb on + strand at 589.201 kb on + strand at 589.276 kb on + strand at 589.276 kb on + strand at 589.277 kb on - strand at 589.277 kb on - strand at 589.278 kb on + strand at 589.278 kb on + strand at 589.340 kb on + strand at 589.357 kb on + strand at 589.357 kb on + strand at 589.357 kb on + strand at 589.358 kb on - strand at 589.465 kb on + strand at 589.465 kb on + strand at 589.682 kb on - strand, within PS417_02590 at 589.682 kb on - strand, within PS417_02590 at 589.733 kb on - strand at 589.775 kb on + strand, within PS417_02590 at 589.823 kb on - strand, within PS417_02590 at 589.990 kb on - strand, within PS417_02590 at 590.236 kb on + strand, within PS417_02590 at 590.368 kb on + strand, within PS417_02590 at 590.368 kb on + strand, within PS417_02590 at 590.369 kb on - strand, within PS417_02590 at 590.410 kb on + strand, within PS417_02590 at 590.434 kb on + strand, within PS417_02590 at 590.434 kb on + strand at 590.636 kb on - strand, within PS417_02590 at 590.837 kb on - strand, within PS417_02590 at 590.837 kb on - strand, within PS417_02590 at 590.837 kb on - strand, within PS417_02590 at 590.944 kb on + strand, within PS417_02590 at 590.945 kb on - strand, within PS417_02590 at 590.945 kb on - strand, within PS417_02590 at 590.945 kb on - strand, within PS417_02590 at 590.945 kb on - strand, within PS417_02590 at 590.974 kb on + strand, within PS417_02590 at 590.974 kb on + strand, within PS417_02590 at 590.975 kb on - strand, within PS417_02590 at 591.002 kb on - strand, within PS417_02590 at 591.013 kb on + strand, within PS417_02590 at 591.013 kb on + strand, within PS417_02590 at 591.013 kb on + strand, within PS417_02590 at 591.014 kb on - strand, within PS417_02590 at 591.148 kb on + strand, within PS417_02590 at 591.148 kb on + strand, within PS417_02590 at 591.184 kb on + strand, within PS417_02590 at 591.185 kb on - strand, within PS417_02590 at 591.373 kb on + strand, within PS417_02590 at 591.374 kb on - strand, within PS417_02590 at 591.374 kb on - strand, within PS417_02590 at 591.642 kb on - strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.887 kb on + strand, within PS417_02595 at 591.887 kb on + strand, within PS417_02595 at 591.888 kb on - strand, within PS417_02595 at 591.888 kb on - strand, within PS417_02595 at 591.984 kb on - strand, within PS417_02595 at 592.169 kb on - strand, within PS417_02595 at 592.219 kb on + strand, within PS417_02595 at 592.219 kb on + strand, within PS417_02595 at 592.219 kb on + strand, within PS417_02595 at 592.220 kb on - strand, within PS417_02595 at 592.220 kb on - strand, within PS417_02595 at 592.223 kb on + strand, within PS417_02595 at 592.223 kb on + strand, within PS417_02595 at 592.224 kb on - strand, within PS417_02595 at 592.224 kb on - strand, within PS417_02595 at 592.224 kb on - strand, within PS417_02595 at 592.277 kb on + strand, within PS417_02595 at 592.295 kb on - strand, within PS417_02595 at 592.454 kb on - strand, within PS417_02595 at 592.486 kb on - strand, within PS417_02595 at 592.609 kb on + strand at 592.609 kb on + strand at 592.610 kb on - strand at 592.610 kb on - strand at 592.610 kb on - strand at 592.623 kb on + strand at 592.624 kb on - strand at 592.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 589,201 + +2.4 589,201 + +1.0 589,276 + +1.0 589,276 + -1.4 589,277 - +0.6 589,277 - -1.7 589,278 + -0.8 589,278 + +0.9 589,340 + -1.6 589,357 + +0.1 589,357 + +1.9 589,357 + -0.6 589,358 - -1.9 589,465 + +0.6 589,465 + -0.6 589,682 - PS417_02590 0.16 -0.9 589,682 - PS417_02590 0.16 -0.2 589,733 - +1.6 589,775 + PS417_02590 0.20 -3.0 589,823 - PS417_02590 0.22 -0.6 589,990 - PS417_02590 0.29 +0.5 590,236 + PS417_02590 0.40 -1.8 590,368 + PS417_02590 0.45 -2.2 590,368 + PS417_02590 0.45 -1.1 590,369 - PS417_02590 0.45 -2.2 590,410 + PS417_02590 0.47 -0.0 590,434 + PS417_02590 0.48 +1.1 590,434 + +0.8 590,636 - PS417_02590 0.57 -0.8 590,837 - PS417_02590 0.66 +0.0 590,837 - PS417_02590 0.66 +1.8 590,837 - PS417_02590 0.66 -1.1 590,944 + PS417_02590 0.70 +1.1 590,945 - PS417_02590 0.70 +0.6 590,945 - PS417_02590 0.70 -0.2 590,945 - PS417_02590 0.70 -0.5 590,945 - PS417_02590 0.70 -0.4 590,974 + PS417_02590 0.72 -0.6 590,974 + PS417_02590 0.72 -1.1 590,975 - PS417_02590 0.72 -0.8 591,002 - PS417_02590 0.73 -1.9 591,013 + PS417_02590 0.73 +0.3 591,013 + PS417_02590 0.73 -0.5 591,013 + PS417_02590 0.73 -0.7 591,014 - PS417_02590 0.73 -2.0 591,148 + PS417_02590 0.79 -1.6 591,148 + PS417_02590 0.79 +0.3 591,184 + PS417_02590 0.81 -0.2 591,185 - PS417_02590 0.81 -2.2 591,373 + PS417_02590 0.89 -0.2 591,374 - PS417_02590 0.89 +0.7 591,374 - PS417_02590 0.89 +0.8 591,642 - -1.0 591,742 + +0.0 591,742 + +1.1 591,742 + +0.9 591,742 + -0.0 591,743 - -1.8 591,743 - -2.9 591,887 + PS417_02595 0.15 -0.1 591,887 + PS417_02595 0.15 +1.9 591,888 - PS417_02595 0.16 +0.3 591,888 - PS417_02595 0.16 +0.2 591,984 - PS417_02595 0.26 -0.1 592,169 - PS417_02595 0.46 -3.5 592,219 + PS417_02595 0.51 -0.1 592,219 + PS417_02595 0.51 -3.3 592,219 + PS417_02595 0.51 -1.0 592,220 - PS417_02595 0.51 +0.5 592,220 - PS417_02595 0.51 +0.6 592,223 + PS417_02595 0.51 +0.2 592,223 + PS417_02595 0.51 +1.8 592,224 - PS417_02595 0.52 +0.6 592,224 - PS417_02595 0.52 +0.7 592,224 - PS417_02595 0.52 +3.1 592,277 + PS417_02595 0.57 -0.5 592,295 - PS417_02595 0.59 +0.6 592,454 - PS417_02595 0.76 +0.6 592,486 - PS417_02595 0.80 -2.4 592,609 + +0.3 592,609 + -0.6 592,610 - +0.2 592,610 - +0.9 592,610 - +1.0 592,623 + -0.5 592,624 - -1.0 592,624 - +1.9
Or see this region's nucleotide sequence