Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25775 and PS417_25780 are separated by 71 nucleotides PS417_25780 and PS417_25785 are separated by 1 nucleotides PS417_25785 and PS417_25790 overlap by 4 nucleotides
PS417_25775: PS417_25775 - lipoprotein, at 5,565,103 to 5,566,077
_25775
PS417_25780: PS417_25780 - molecular chaperone DnaJ, at 5,566,149 to 5,566,916
_25780
PS417_25785: PS417_25785 - mannose-1-phosphate guanylyltransferase, at 5,566,918 to 5,567,592
_25785
PS417_25790: PS417_25790 - aminoglycoside phosphotransferase, at 5,567,589 to 5,568,614
_25790
Position (kb) 5566
5567 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5565.372 kb on + strand, within PS417_25775 at 5565.377 kb on + strand, within PS417_25775 at 5565.417 kb on + strand, within PS417_25775 at 5565.418 kb on - strand, within PS417_25775 at 5565.492 kb on + strand at 5565.493 kb on - strand, within PS417_25775 at 5565.509 kb on + strand, within PS417_25775 at 5565.509 kb on + strand, within PS417_25775 at 5565.726 kb on - strand at 5565.849 kb on - strand, within PS417_25775 at 5565.849 kb on - strand, within PS417_25775 at 5565.849 kb on - strand, within PS417_25775 at 5565.895 kb on + strand, within PS417_25775 at 5565.896 kb on - strand, within PS417_25775 at 5565.971 kb on - strand, within PS417_25775 at 5566.031 kb on - strand at 5566.127 kb on + strand at 5566.183 kb on + strand at 5566.184 kb on - strand at 5566.205 kb on + strand at 5566.357 kb on + strand, within PS417_25780 at 5566.362 kb on - strand, within PS417_25780 at 5566.527 kb on + strand, within PS417_25780 at 5566.566 kb on + strand, within PS417_25780 at 5566.567 kb on - strand, within PS417_25780 at 5566.570 kb on + strand, within PS417_25780 at 5567.593 kb on - strand at 5567.593 kb on - strand at 5567.593 kb on - strand at 5567.593 kb on - strand at 5567.677 kb on - strand at 5567.678 kb on + strand at 5567.679 kb on - strand at 5567.679 kb on - strand at 5567.680 kb on + strand at 5567.680 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,565,372 + PS417_25775 0.28 +1.9 5,565,377 + PS417_25775 0.28 -1.0 5,565,417 + PS417_25775 0.32 -0.7 5,565,418 - PS417_25775 0.32 -0.2 5,565,492 + -0.2 5,565,493 - PS417_25775 0.40 +0.5 5,565,509 + PS417_25775 0.42 +0.2 5,565,509 + PS417_25775 0.42 +0.8 5,565,726 - +0.2 5,565,849 - PS417_25775 0.77 -0.5 5,565,849 - PS417_25775 0.77 +0.7 5,565,849 - PS417_25775 0.77 -0.1 5,565,895 + PS417_25775 0.81 -0.8 5,565,896 - PS417_25775 0.81 -0.9 5,565,971 - PS417_25775 0.89 +1.4 5,566,031 - +1.3 5,566,127 + +1.0 5,566,183 + -1.9 5,566,184 - -2.5 5,566,205 + +1.8 5,566,357 + PS417_25780 0.27 -2.8 5,566,362 - PS417_25780 0.28 -0.5 5,566,527 + PS417_25780 0.49 -1.2 5,566,566 + PS417_25780 0.54 -0.3 5,566,567 - PS417_25780 0.54 -0.5 5,566,570 + PS417_25780 0.55 -2.2 5,567,593 - -1.9 5,567,593 - -1.2 5,567,593 - -2.0 5,567,593 - -0.5 5,567,677 - -3.1 5,567,678 + -2.2 5,567,679 - -2.9 5,567,679 - -1.0 5,567,680 + -4.9 5,567,680 + -4.0
Or see this region's nucleotide sequence