Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25675 and PS417_25680 are separated by 63 nucleotides PS417_25680 and PS417_25685 are separated by 46 nucleotides PS417_25685 and PS417_25690 are separated by 116 nucleotides PS417_25690 and PS417_25695 are separated by 61 nucleotides
PS417_25675: PS417_25675 - membrane protein, at 5,545,555 to 5,546,511
_25675
PS417_25680: PS417_25680 - short-chain dehydrogenase, at 5,546,575 to 5,547,363
_25680
PS417_25685: PS417_25685 - zinc-binding protein, at 5,547,410 to 5,547,748
_25685
PS417_25690: PS417_25690 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 5,547,865 to 5,548,512
_25690
PS417_25695: PS417_25695 - peroxiredoxin, at 5,548,574 to 5,548,996
_25695
Position (kb) 5547
5548 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5546.492 kb on + strand at 5546.493 kb on - strand at 5547.028 kb on + strand at 5547.028 kb on + strand, within PS417_25680 at 5547.029 kb on - strand, within PS417_25680 at 5547.029 kb on - strand, within PS417_25680 at 5547.032 kb on - strand at 5547.032 kb on - strand, within PS417_25680 at 5547.046 kb on + strand, within PS417_25680 at 5547.046 kb on + strand, within PS417_25680 at 5547.046 kb on + strand, within PS417_25680 at 5547.047 kb on - strand, within PS417_25680 at 5547.047 kb on - strand, within PS417_25680 at 5547.047 kb on - strand, within PS417_25680 at 5547.047 kb on - strand, within PS417_25680 at 5547.217 kb on + strand, within PS417_25680 at 5547.217 kb on + strand, within PS417_25680 at 5547.218 kb on - strand, within PS417_25680 at 5547.218 kb on - strand, within PS417_25680 at 5547.349 kb on + strand at 5547.350 kb on - strand at 5547.373 kb on + strand at 5547.374 kb on - strand at 5547.394 kb on + strand at 5547.395 kb on - strand at 5547.447 kb on + strand, within PS417_25685 at 5547.447 kb on + strand, within PS417_25685 at 5547.448 kb on - strand, within PS417_25685 at 5547.465 kb on - strand, within PS417_25685 at 5547.465 kb on - strand, within PS417_25685 at 5547.577 kb on + strand, within PS417_25685 at 5547.578 kb on - strand, within PS417_25685 at 5547.595 kb on + strand, within PS417_25685 at 5547.595 kb on + strand, within PS417_25685 at 5547.595 kb on + strand, within PS417_25685 at 5547.595 kb on + strand, within PS417_25685 at 5547.595 kb on + strand, within PS417_25685 at 5547.596 kb on - strand, within PS417_25685 at 5547.703 kb on + strand, within PS417_25685 at 5547.703 kb on + strand, within PS417_25685 at 5547.703 kb on + strand, within PS417_25685 at 5547.704 kb on - strand, within PS417_25685 at 5547.704 kb on - strand, within PS417_25685 at 5547.704 kb on - strand at 5547.763 kb on + strand at 5548.597 kb on - strand at 5548.651 kb on - strand, within PS417_25695 at 5548.651 kb on - strand, within PS417_25695 at 5548.742 kb on + strand, within PS417_25695 at 5548.743 kb on - strand, within PS417_25695 at 5548.743 kb on - strand, within PS417_25695
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,546,492 + -1.5 5,546,493 - +0.5 5,547,028 + -1.0 5,547,028 + PS417_25680 0.57 -1.8 5,547,029 - PS417_25680 0.58 +0.2 5,547,029 - PS417_25680 0.58 -2.5 5,547,032 - -0.8 5,547,032 - PS417_25680 0.58 -0.5 5,547,046 + PS417_25680 0.60 +2.1 5,547,046 + PS417_25680 0.60 +0.3 5,547,046 + PS417_25680 0.60 -0.4 5,547,047 - PS417_25680 0.60 -1.4 5,547,047 - PS417_25680 0.60 -0.1 5,547,047 - PS417_25680 0.60 +0.5 5,547,047 - PS417_25680 0.60 -0.2 5,547,217 + PS417_25680 0.81 -0.1 5,547,217 + PS417_25680 0.81 +0.0 5,547,218 - PS417_25680 0.81 +0.5 5,547,218 - PS417_25680 0.81 +2.0 5,547,349 + +0.1 5,547,350 - -2.2 5,547,373 + +1.7 5,547,374 - +0.1 5,547,394 + -2.9 5,547,395 - -0.5 5,547,447 + PS417_25685 0.11 +0.5 5,547,447 + PS417_25685 0.11 -0.8 5,547,448 - PS417_25685 0.11 -3.0 5,547,465 - PS417_25685 0.16 +0.2 5,547,465 - PS417_25685 0.16 +1.7 5,547,577 + PS417_25685 0.49 +0.5 5,547,578 - PS417_25685 0.50 +0.4 5,547,595 + PS417_25685 0.55 -2.8 5,547,595 + PS417_25685 0.55 +2.1 5,547,595 + PS417_25685 0.55 -1.7 5,547,595 + PS417_25685 0.55 -0.2 5,547,595 + PS417_25685 0.55 +0.6 5,547,596 - PS417_25685 0.55 +1.7 5,547,703 + PS417_25685 0.86 -0.1 5,547,703 + PS417_25685 0.86 +0.0 5,547,703 + PS417_25685 0.86 +0.7 5,547,704 - PS417_25685 0.87 +2.1 5,547,704 - PS417_25685 0.87 +0.7 5,547,704 - +0.8 5,547,763 + +1.0 5,548,597 - -0.9 5,548,651 - PS417_25695 0.18 +0.4 5,548,651 - PS417_25695 0.18 +1.5 5,548,742 + PS417_25695 0.40 +1.8 5,548,743 - PS417_25695 0.40 +0.5 5,548,743 - PS417_25695 0.40 +0.3
Or see this region's nucleotide sequence