Strain Fitness in Pseudomonas simiae WCS417 around PS417_25660

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_25650 and PS417_25655 are separated by 77 nucleotidesPS417_25655 and PS417_25660 are separated by 165 nucleotidesPS417_25660 and PS417_25665 are separated by 136 nucleotidesPS417_25665 and PS417_25670 are separated by 24 nucleotides PS417_25650: PS417_25650 - anhydro-N-acetylmuramic acid kinase, at 5,541,213 to 5,542,304 _25650 PS417_25655: PS417_25655 - iron--sulfur cluster insertion protein ErpA, at 5,542,382 to 5,542,732 _25655 PS417_25660: PS417_25660 - N-acetyl-gamma-glutamyl-phosphate reductase, at 5,542,898 to 5,543,932 _25660 PS417_25665: PS417_25665 - membrane protein, at 5,544,069 to 5,544,497 _25665 PS417_25670: PS417_25670 - 2-nitropropane dioxygenase, at 5,544,522 to 5,545,484 _25670 Position (kb) 5542 5543 5544Strain fitness (log2 ratio) -2 -1 0 1 2at 5542.369 kb on + strandat 5542.769 kb on - strandat 5542.769 kb on - strandat 5542.823 kb on + strandat 5542.824 kb on - strandat 5542.824 kb on - strandat 5542.836 kb on - strandat 5542.900 kb on - strandat 5542.900 kb on - strandat 5542.900 kb on - strandat 5543.238 kb on - strand, within PS417_25660at 5543.796 kb on + strand, within PS417_25660at 5543.933 kb on - strandat 5543.973 kb on + strandat 5543.973 kb on + strandat 5544.012 kb on + strandat 5544.013 kb on - strandat 5544.031 kb on - strandat 5544.033 kb on + strandat 5544.033 kb on + strandat 5544.033 kb on + strandat 5544.033 kb on + strandat 5544.034 kb on - strandat 5544.034 kb on - strandat 5544.034 kb on - strandat 5544.034 kb on - strandat 5544.034 kb on - strandat 5544.064 kb on + strandat 5544.496 kb on - strandat 5544.496 kb on - strandat 5544.505 kb on + strandat 5544.825 kb on - strand, within PS417_25670at 5544.853 kb on + strand, within PS417_25670at 5544.854 kb on - strand, within PS417_25670at 5544.862 kb on + strand, within PS417_25670

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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5,542,369 + -0.6
5,542,769 - +0.4
5,542,769 - -1.1
5,542,823 + -2.4
5,542,824 - -1.7
5,542,824 - -0.4
5,542,836 - +1.5
5,542,900 - +1.9
5,542,900 - -0.1
5,542,900 - +1.1
5,543,238 - PS417_25660 0.33 -0.4
5,543,796 + PS417_25660 0.87 -1.7
5,543,933 - -2.3
5,543,973 + -1.5
5,543,973 + -0.7
5,544,012 + +0.4
5,544,013 - +0.8
5,544,031 - -0.6
5,544,033 + -0.8
5,544,033 + -0.8
5,544,033 + -0.3
5,544,033 + +1.0
5,544,034 - +0.1
5,544,034 - -1.5
5,544,034 - +2.2
5,544,034 - +0.3
5,544,034 - +2.1
5,544,064 + -0.8
5,544,496 - -1.9
5,544,496 - -0.0
5,544,505 + -1.0
5,544,825 - PS417_25670 0.31 +0.8
5,544,853 + PS417_25670 0.34 +0.7
5,544,854 - PS417_25670 0.34 -1.4
5,544,862 + PS417_25670 0.35 -2.6

Or see this region's nucleotide sequence