Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25650 and PS417_25655 are separated by 77 nucleotides PS417_25655 and PS417_25660 are separated by 165 nucleotides PS417_25660 and PS417_25665 are separated by 136 nucleotides PS417_25665 and PS417_25670 are separated by 24 nucleotides
PS417_25650: PS417_25650 - anhydro-N-acetylmuramic acid kinase, at 5,541,213 to 5,542,304
_25650
PS417_25655: PS417_25655 - iron--sulfur cluster insertion protein ErpA, at 5,542,382 to 5,542,732
_25655
PS417_25660: PS417_25660 - N-acetyl-gamma-glutamyl-phosphate reductase, at 5,542,898 to 5,543,932
_25660
PS417_25665: PS417_25665 - membrane protein, at 5,544,069 to 5,544,497
_25665
PS417_25670: PS417_25670 - 2-nitropropane dioxygenase, at 5,544,522 to 5,545,484
_25670
Position (kb)
5542
5543
5544 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5542.369 kb on + strand at 5542.769 kb on - strand at 5542.769 kb on - strand at 5542.823 kb on + strand at 5542.824 kb on - strand at 5542.824 kb on - strand at 5542.836 kb on - strand at 5542.900 kb on - strand at 5542.900 kb on - strand at 5542.900 kb on - strand at 5543.238 kb on - strand, within PS417_25660 at 5543.796 kb on + strand, within PS417_25660 at 5543.933 kb on - strand at 5543.973 kb on + strand at 5543.973 kb on + strand at 5544.012 kb on + strand at 5544.013 kb on - strand at 5544.031 kb on - strand at 5544.033 kb on + strand at 5544.033 kb on + strand at 5544.033 kb on + strand at 5544.033 kb on + strand at 5544.034 kb on - strand at 5544.034 kb on - strand at 5544.034 kb on - strand at 5544.034 kb on - strand at 5544.034 kb on - strand at 5544.064 kb on + strand at 5544.496 kb on - strand at 5544.496 kb on - strand at 5544.505 kb on + strand at 5544.825 kb on - strand, within PS417_25670 at 5544.853 kb on + strand, within PS417_25670 at 5544.854 kb on - strand, within PS417_25670 at 5544.862 kb on + strand, within PS417_25670
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,542,369 + -0.6 5,542,769 - +0.4 5,542,769 - -1.1 5,542,823 + -2.4 5,542,824 - -1.7 5,542,824 - -0.4 5,542,836 - +1.5 5,542,900 - +1.9 5,542,900 - -0.1 5,542,900 - +1.1 5,543,238 - PS417_25660 0.33 -0.4 5,543,796 + PS417_25660 0.87 -1.7 5,543,933 - -2.3 5,543,973 + -1.5 5,543,973 + -0.7 5,544,012 + +0.4 5,544,013 - +0.8 5,544,031 - -0.6 5,544,033 + -0.8 5,544,033 + -0.8 5,544,033 + -0.3 5,544,033 + +1.0 5,544,034 - +0.1 5,544,034 - -1.5 5,544,034 - +2.2 5,544,034 - +0.3 5,544,034 - +2.1 5,544,064 + -0.8 5,544,496 - -1.9 5,544,496 - -0.0 5,544,505 + -1.0 5,544,825 - PS417_25670 0.31 +0.8 5,544,853 + PS417_25670 0.34 +0.7 5,544,854 - PS417_25670 0.34 -1.4 5,544,862 + PS417_25670 0.35 -2.6
Or see this region's nucleotide sequence