Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25215 and PS417_25220 are separated by 43 nucleotides PS417_25220 and PS417_25225 overlap by 4 nucleotides PS417_25225 and PS417_25230 overlap by 4 nucleotides PS417_25230 and PS417_25235 are separated by 150 nucleotides
PS417_25215: PS417_25215 - hypothetical protein, at 5,466,396 to 5,466,602
_25215
PS417_25220: PS417_25220 - iron-hydroxamate ABC transporter substrate-binding protein, at 5,466,646 to 5,467,434
_25220
PS417_25225: PS417_25225 - MFS transporter, at 5,467,431 to 5,467,850
_25225
PS417_25230: PS417_25230 - GTP cyclohydrolase, at 5,467,847 to 5,468,464
_25230
PS417_25235: PS417_25235 - ABC transporter substrate-binding protein, at 5,468,615 to 5,469,334
_25235
Position (kb)
5467
5468 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5466.655 kb on - strand at 5466.933 kb on + strand, within PS417_25220 at 5466.936 kb on - strand, within PS417_25220 at 5466.936 kb on - strand, within PS417_25220 at 5466.953 kb on - strand, within PS417_25220 at 5467.107 kb on + strand, within PS417_25220 at 5467.213 kb on + strand, within PS417_25220 at 5467.438 kb on - strand at 5467.462 kb on + strand at 5467.462 kb on + strand at 5467.463 kb on - strand at 5467.463 kb on - strand at 5467.658 kb on + strand, within PS417_25225 at 5467.658 kb on + strand, within PS417_25225 at 5467.802 kb on - strand, within PS417_25225 at 5468.505 kb on + strand at 5468.506 kb on - strand at 5468.548 kb on + strand at 5468.587 kb on - strand at 5468.587 kb on - strand at 5468.591 kb on - strand at 5468.616 kb on + strand at 5468.616 kb on + strand at 5468.617 kb on - strand at 5468.617 kb on - strand at 5468.617 kb on - strand at 5468.700 kb on - strand at 5468.700 kb on - strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.746 kb on - strand, within PS417_25235 at 5468.811 kb on + strand, within PS417_25235 at 5468.812 kb on - strand, within PS417_25235 at 5468.812 kb on - strand, within PS417_25235 at 5468.838 kb on + strand, within PS417_25235 at 5468.839 kb on - strand, within PS417_25235 at 5468.839 kb on - strand, within PS417_25235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,466,655 - -0.9 5,466,933 + PS417_25220 0.36 -0.2 5,466,936 - PS417_25220 0.37 +0.3 5,466,936 - PS417_25220 0.37 -1.4 5,466,953 - PS417_25220 0.39 -0.8 5,467,107 + PS417_25220 0.58 +0.3 5,467,213 + PS417_25220 0.72 -0.9 5,467,438 - -0.3 5,467,462 + +2.3 5,467,462 + -0.6 5,467,463 - -0.9 5,467,463 - -1.9 5,467,658 + PS417_25225 0.54 +0.8 5,467,658 + PS417_25225 0.54 -2.3 5,467,802 - PS417_25225 0.88 -2.3 5,468,505 + -0.5 5,468,506 - +1.3 5,468,548 + -3.2 5,468,587 - -1.4 5,468,587 - -0.7 5,468,591 - +0.2 5,468,616 + +2.6 5,468,616 + -0.6 5,468,617 - -1.2 5,468,617 - -1.7 5,468,617 - +1.4 5,468,700 - +0.8 5,468,700 - PS417_25235 0.12 -0.8 5,468,745 + PS417_25235 0.18 +0.4 5,468,745 + PS417_25235 0.18 +0.6 5,468,745 + PS417_25235 0.18 -1.2 5,468,745 + PS417_25235 0.18 +1.0 5,468,745 + PS417_25235 0.18 +0.2 5,468,746 - PS417_25235 0.18 +0.2 5,468,811 + PS417_25235 0.27 -1.4 5,468,812 - PS417_25235 0.27 +1.1 5,468,812 - PS417_25235 0.27 -1.5 5,468,838 + PS417_25235 0.31 -1.1 5,468,839 - PS417_25235 0.31 +1.2 5,468,839 - PS417_25235 0.31 -0.2
Or see this region's nucleotide sequence