Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02460 and PS417_02465 are separated by 63 nucleotides PS417_02465 and PS417_02470 overlap by 13 nucleotides PS417_02470 and PS417_02475 are separated by 15 nucleotides
PS417_02460: PS417_02460 - epoxyqueuosine reductase, at 562,616 to 563,680
_02460
PS417_02465: PS417_02465 - carbohydrate kinase, at 563,744 to 564,604
_02465
PS417_02470: PS417_02470 - ATPase, at 564,592 to 565,062
_02470
PS417_02475: PS417_02475 - N-acetylmuramoyl-L-alanine amidase, at 565,078 to 566,499
_02475
Position (kb)
563
564
565 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 562.828 kb on + strand, within PS417_02460 at 562.828 kb on + strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 563.041 kb on - strand, within PS417_02460 at 563.167 kb on - strand, within PS417_02460 at 563.190 kb on - strand, within PS417_02460 at 563.379 kb on + strand, within PS417_02460 at 563.380 kb on - strand, within PS417_02460 at 563.402 kb on + strand, within PS417_02460 at 563.434 kb on - strand, within PS417_02460 at 563.434 kb on - strand, within PS417_02460 at 563.517 kb on + strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460 at 563.713 kb on - strand at 563.775 kb on + strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.862 kb on + strand, within PS417_02465 at 563.862 kb on + strand, within PS417_02465 at 563.862 kb on + strand, within PS417_02465 at 563.862 kb on + strand, within PS417_02465 at 563.863 kb on - strand, within PS417_02465 at 564.275 kb on - strand, within PS417_02465 at 564.465 kb on - strand, within PS417_02465 at 564.555 kb on + strand at 564.602 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 562,828 + PS417_02460 0.20 +0.2 562,828 + PS417_02460 0.20 +0.0 562,829 - PS417_02460 0.20 +0.2 562,829 - PS417_02460 0.20 +1.8 562,829 - PS417_02460 0.20 +0.2 563,041 - PS417_02460 0.40 -0.3 563,167 - PS417_02460 0.52 -0.1 563,190 - PS417_02460 0.54 -0.1 563,379 + PS417_02460 0.72 -0.4 563,380 - PS417_02460 0.72 +0.6 563,402 + PS417_02460 0.74 -0.6 563,434 - PS417_02460 0.77 +0.2 563,434 - PS417_02460 0.77 +2.2 563,517 + PS417_02460 0.85 -1.2 563,518 - PS417_02460 0.85 -0.5 563,518 - PS417_02460 0.85 -2.1 563,518 - PS417_02460 0.85 +1.0 563,713 - -0.9 563,775 + -1.3 563,776 - +1.4 563,776 - +0.1 563,776 - -1.4 563,776 - -1.6 563,776 - -0.5 563,776 - +0.1 563,862 + PS417_02465 0.14 -0.1 563,862 + PS417_02465 0.14 +0.3 563,862 + PS417_02465 0.14 -0.6 563,862 + PS417_02465 0.14 -2.9 563,863 - PS417_02465 0.14 -1.6 564,275 - PS417_02465 0.62 -0.4 564,465 - PS417_02465 0.84 +0.2 564,555 + +0.7 564,602 + +1.0
Or see this region's nucleotide sequence