Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24645 and PS417_24650 are separated by 126 nucleotides PS417_24650 and PS417_24655 are separated by 99 nucleotides PS417_24655 and PS417_24660 are separated by 262 nucleotides
PS417_24645: PS417_24645 - acyltransferase, at 5,347,213 to 5,348,736
_24645
PS417_24650: PS417_24650 - membrane protein, at 5,348,863 to 5,349,624
_24650
PS417_24655: PS417_24655 - hypothetical protein, at 5,349,724 to 5,350,041
_24655
PS417_24660: PS417_24660 - leucine--tRNA ligase, at 5,350,304 to 5,352,910
_24660
Position (kb)
5348
5349
5350 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5349.088 kb on - strand, within PS417_24650 at 5349.100 kb on + strand at 5349.100 kb on + strand, within PS417_24650 at 5349.611 kb on + strand at 5349.637 kb on + strand at 5349.637 kb on + strand at 5349.637 kb on + strand at 5349.652 kb on + strand at 5349.653 kb on - strand at 5349.682 kb on + strand at 5349.692 kb on + strand at 5349.693 kb on - strand at 5349.726 kb on - strand at 5349.726 kb on - strand at 5349.869 kb on + strand, within PS417_24655 at 5349.869 kb on + strand, within PS417_24655 at 5350.001 kb on + strand, within PS417_24655 at 5350.001 kb on + strand, within PS417_24655 at 5350.001 kb on + strand, within PS417_24655 at 5350.002 kb on - strand, within PS417_24655 at 5350.061 kb on + strand at 5350.061 kb on + strand at 5350.062 kb on - strand at 5350.062 kb on - strand at 5350.173 kb on + strand at 5350.173 kb on + strand at 5350.173 kb on + strand at 5350.173 kb on + strand at 5350.173 kb on + strand at 5350.173 kb on + strand at 5350.173 kb on + strand at 5350.173 kb on + strand at 5350.173 kb on + strand at 5350.284 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,349,088 - PS417_24650 0.30 -2.5 5,349,100 + -0.2 5,349,100 + PS417_24650 0.31 -1.3 5,349,611 + +0.0 5,349,637 + +1.6 5,349,637 + +1.2 5,349,637 + +0.7 5,349,652 + -1.6 5,349,653 - +2.1 5,349,682 + -0.5 5,349,692 + -2.3 5,349,693 - +2.4 5,349,726 - -1.5 5,349,726 - +0.2 5,349,869 + PS417_24655 0.46 +1.1 5,349,869 + PS417_24655 0.46 -0.9 5,350,001 + PS417_24655 0.87 +0.3 5,350,001 + PS417_24655 0.87 -0.4 5,350,001 + PS417_24655 0.87 +1.8 5,350,002 - PS417_24655 0.87 +0.7 5,350,061 + -1.4 5,350,061 + -0.2 5,350,062 - -0.1 5,350,062 - -1.0 5,350,173 + -0.8 5,350,173 + +0.2 5,350,173 + -0.3 5,350,173 + -0.8 5,350,173 + +1.8 5,350,173 + +0.2 5,350,173 + +1.6 5,350,173 + +0.9 5,350,173 + +0.6 5,350,284 + -0.3
Or see this region's nucleotide sequence