Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24415 and PS417_24420 are separated by 63 nucleotides PS417_24420 and PS417_24430 are separated by 206 nucleotides PS417_24430 and PS417_24435 overlap by 4 nucleotides PS417_24435 and PS417_24440 are separated by 133 nucleotides
PS417_24415: PS417_24415 - siderophore-interacting protein, at 5,302,120 to 5,303,118
_24415
PS417_24420: PS417_24420 - RNA polymerase sigma70, at 5,303,182 to 5,303,703
_24420
PS417_24430: PS417_24430 - 3-dehydroquinate dehydratase, at 5,303,910 to 5,304,359
_24430
PS417_24435: PS417_24435 - shikimate dehydrogenase, at 5,304,356 to 5,305,204
_24435
PS417_24440: PS417_24440 - TetR family transcriptional regulator, at 5,305,338 to 5,305,979
_24440
Position (kb) 5303
5304
5305 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5302.932 kb on - strand, within PS417_24415 at 5303.086 kb on - strand at 5303.141 kb on + strand at 5303.142 kb on - strand at 5303.145 kb on + strand at 5303.145 kb on + strand at 5303.145 kb on + strand at 5303.146 kb on - strand at 5303.231 kb on + strand at 5303.232 kb on - strand at 5303.529 kb on - strand, within PS417_24420 at 5303.743 kb on + strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.980 kb on - strand, within PS417_24430 at 5304.340 kb on + strand at 5304.341 kb on - strand at 5304.558 kb on + strand, within PS417_24435 at 5304.559 kb on + strand, within PS417_24435 at 5304.563 kb on + strand, within PS417_24435 at 5304.817 kb on + strand, within PS417_24435 at 5304.818 kb on - strand, within PS417_24435 at 5305.034 kb on + strand, within PS417_24435 at 5305.198 kb on + strand at 5305.198 kb on + strand at 5305.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,302,932 - PS417_24415 0.81 +0.8 5,303,086 - -1.2 5,303,141 + -0.5 5,303,142 - +1.2 5,303,145 + -2.0 5,303,145 + -3.0 5,303,145 + -0.1 5,303,146 - +2.0 5,303,231 + +0.3 5,303,232 - +0.4 5,303,529 - PS417_24420 0.66 +0.9 5,303,743 + -3.8 5,303,892 - +2.6 5,303,892 - -2.5 5,303,892 - -0.5 5,303,892 - -0.2 5,303,892 - -2.0 5,303,980 - PS417_24430 0.16 -1.3 5,304,340 + +0.4 5,304,341 - -0.2 5,304,558 + PS417_24435 0.24 +0.3 5,304,559 + PS417_24435 0.24 -0.2 5,304,563 + PS417_24435 0.24 -0.7 5,304,817 + PS417_24435 0.54 +1.0 5,304,818 - PS417_24435 0.54 +0.4 5,305,034 + PS417_24435 0.80 +1.2 5,305,198 + -0.5 5,305,198 + +1.6 5,305,335 + -1.0
Or see this region's nucleotide sequence